Food Science of Animal Resources (한국축산식품학회지)

Korean Society for Food Science of Animal Resources (한국축산식품학회)
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Effects of Ethanol Extracts of Bacillus polyfermenticus SCD on Tteokgalbi Quality during Storage

Bacillus polyfermenticus SCD 에탄올 추출물이 떡갈비의 품질 및 저장성에 미치는 영향

  • Kim, Hack-Youn (Department of Food Science and Biotechnology of Animal Resources, Konkuk University) ;
  • Jeong, Jong-Youn (Department of Food Science and Biotechnology of Animal Resources, Konkuk University) ;
  • Choi, Ji-Hun (Department of Food Science and Biotechnology of Animal Resources, Konkuk University) ;
  • Lee, Mi-Ai (Department of Food Science and Biotechnology of Animal Resources, Konkuk University) ;
  • Lee, Jang-Hyun (Department of Food Science and Biotechnology of Animal Resources, Konkuk University) ;
  • Chang, Kyung-Hoon (Department of Food Science and Biotechnology of Animal Resources, Konkuk University) ;
  • Choi, Shin-Yang (Korea Food Research Institute) ;
  • Paik, Hyun-Dong (Department of Food Science and Biotechnology of Animal Resources, Konkuk University) ;
  • Kim, Cheon-Jei (Department of Food Science and Biotechnology of Animal Resources, Konkuk University)
  • Published : 2006.12.31
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Abstract

The treatments of Tteokgalbi studied were: control (no antioxidants); (T1) ethanol extracts of Bacillus polyfermenticus SCD (5%); (T2) ascorbic acid (0.06%); (T3) ascorbic acid (0.03%) + ethanol extracts of B. polyfermenticus SCD (2.5%). The pH of T1, T2, and T3 samples was significantly (p<0.05) lower than that of the control for up to 3 days of storage. Thereafter T1, T2 and T3 had a significantly higher (p<0.05) pH value than the control during storage. TBA values were significantly lower (p<0.05) in all treated samples relative to the control. The TBA value of the control rapidly increased after 6 days of storage, whereas the TBA values of the test samples did not sharply increase. T3 samples treated with vitamin C and Bacillus polyfermenticus SCD had a higher TBA value than T1 and T2 samples. The VBN values of T1, T2 and T3 sampleswere lower than that of the control (p>0.05). VBN values of the ground pork meat samples significantly increased (p<0.05) with storage time. The total microbial counts of each sample significantly increased with storage time (p<0.05). The $a^*$ values of T1 and T3 samples containing added vitamin C were significantly higher (p<0.05) than that of the control and T2 samples during storage. The $b^*$ value of T2 samples was significantly higher (p<0.05) than that of other ground pork meat products during storage.

본 연구에서 대조구는 항산화제를 첨가하지 않았고, ascorbic acid(0.06%)를 첨가한 처리구(T1), B. polyfermenticus SCD 에탄을 추출물(5%)을 첨가한 처리구(T2)와 ascorbic acid(0.03%) 및 B. polyfermenticus SCD 에탄을 추출물(2.5%)를 첨가한 처리구(T3)로 구분하여 실험을 실시하였다. 비타민 C 혹은 B. polyfermenticus SCD의 ethanol 추출액을 첨가한 떡갈비는 저장성이 향상되었다. 특히 지질산패도의 경우 처리구들은 대조구에 비하여 현저히 낮은 값을 보였을 뿐만 아니라, B. polyfermenticus SCD의 에탄을 추출물을 첨가한 처리구가 가장 낮은 값을 나타냈다. 휘발성 염기태질소값은 항산화제를 넣은 모든 처리구에서 낮은 결과를 보였다. 따라서, 기존에 많은 연구를 통해 항산화제로 많이 쓰여지고 있는 비타민 C와 더불어 B. polyfermenticus SCD의 에탄올 추출물이 뛰어난 천연항산화 능력을 보였다

Keywords

References

  1. Chang, S. S., Matijasevic, B. O., Hsieh, O. L., and Huang, C. L. (1997) Natural antioxidants from rosmary and sags. J. Food Sci. 42, 1102-1106 https://doi.org/10.1111/j.1365-2621.1977.tb12676.x
  2. Choi, S. H., Kwon, H. C., An, D. J., Park, J. R., and Oh, D. H. (2003) Nitrite contents and storage properties of sausage added with green tea powder. Kor. J. Food Sci. Ani. Resour. 23, 299-308
  3. Djenane, D., Sanchez-Escalante, A, Beltran, J. A, and Roncales, P. (2001) Extension of retail display life of fresh beef packaged in modified atmosphere by varying lighting conditions. J Food Sci. 66, 181-186 https://doi.org/10.1111/j.1365-2621.2001.tb15603.x
  4. Egan, A F., Ford, A L., and Shay, B. J. (1980) A comparison of Brochothrix thermosphacta and Lactobacilli as a spoilage organism of vacuum packaged sliced luncheon meats. J. Food Sci. 45, 1745-1748 https://doi.org/10.1111/j.1365-2621.1980.tb07602.x
  5. Elliott, C. O. (1983) Meat spoilage and evaluation of the potential storage life of fresh meat. J. Food Protect. 46, 444-452 https://doi.org/10.4315/0362-028X-46.5.444
  6. Food Code. (2002) Meat product. Korea Food & Drug Administration. p. 220
  7. Goddard, B. L., Mikel, W. B., Conner, D. E., and Jones, W. R. (1996) Use of organic acids to improve the chemical, physical, and microbial attributes of beef strip loins stored at $-1^{\circ}C$ for 112 days. J. Food Protect. 59, 849-853 https://doi.org/10.4315/0362-028X-59.8.849
  8. Heddur, M. J, Yoon, Y. C., and Paik, H. D. (2005) Probiotic funtional dairy foods and health claims: An overview. Food Sci. Biotechnol. 14. 523-528
  9. Hettiarachchy, N. S., Glenn, K. C., Gnanasambandam, R., and Johnson, M. G. (1996) Natural antioxidant extracts from fenugreek (Trigonella foemumgraecum) for ground beef patties. J. Food Sci. 61, 516-519 https://doi.org/10.1111/j.1365-2621.1996.tb13146.x
  10. Higdon, J. V. and Frei, B. (2003) Tea catdchins and polyphenols: health effects, metabolism, and antioxidant functions. Crit. Rev. Food Sci. Nutr. 43, 89-143 https://doi.org/10.1080/10408690390826464
  11. Ho, C. P., McMillin, K. W., and Huang, N. Y. (1996) Ground beef lipid, color and microsomal stability in gas exchange modified atmosphere packaging. Proceedings, 1996 IFT annual meeting, New Orleans, LA, USA p. 162
  12. Jun, K. D., Lee, K. H., Lee, Kim, W. S., and Paik, H. D. (2000) Microbiological identification of medical probiotic biapan strain. Kor. J. Appl. Microbiol. Biotechnol. 28, 124-127
  13. Jung, I. C., Kang, S. J., Kim, M. S., Yang, J. B., and Moon, Y. H. (2003) Effects of carcass grade and addition of mugwort powder on the storage stability of pork sausage. Kor. J. Food Sci. Ani. Resour. 23, 285-291
  14. Kailasapathy, K. and Chin, J. (2000) Survial and therapeutic potential of probiotic organisms with reference to Lactobacillus acidophilus and Bifidobacterium spp. Immunol. Cell BioI. 78, 80-88 https://doi.org/10.1046/j.1440-1711.2000.00886.x
  15. Kim, G. I. (1999) Effects of added pork and lard on quality and shelf life of beef hamburger patties. Master thesis, Konkuk Univ., Seoul, Korea
  16. Kim, I. H., Park, M. S., and Ji, G. E. (2003) Characterization of adhesion of Bifidobacterium sp. BGN4 to human enterocyte-like Caco-2 cells. J. Microbiol. 13, 276-281
  17. Kim, W. J., Hong, S. S., and Cha, S. K. (1994) Selection of human-originate Lactobacillus acidophilus for production of probiotics. Kor. J. Microbiol. Biotechnol. 4, 151-154
  18. Kimoto, H., Kurisaki, J., Tsuji, N. M., Ohmomo, S., and Okamoto, T. (1999) Lactococci as probiotic strains: adhesion to human enterocyte like Caco-2 cells and tolerance to low pH and bile. Left. Appl. Microbiol. 29, 313-316 https://doi.org/10.1046/j.1365-2672.1999.00627.x
  19. Koo, Y. B. (1989) Effects of cooking and packaging method on physico-chemical and rheological properties of hamburger patties during frozen storage periods. Master thesis, Konkuk Univ., Seoul, Korea
  20. Kopp-Hoolihan, L. (2001) Prophylactic uses of probiotics: A review. J. Am. Diet Assoc. 101, 229-329 https://doi.org/10.1016/S0002-8223(01)00060-8
  21. Lamkey, J. W., Leak, F. W., Tuley, W. B., Johonson, D. D., and West, R. L. (1991) Assessment of sodium lactate addition to fresh pork sausage. J. Food Sci. 56, 220-227 https://doi.org/10.1111/j.1365-2621.1991.tb08015.x
  22. Lee, K. H., Jun, K. D., Jun, W. D., Kim, W. S., and Paik, H. D. (2000) Partial characterization of polyfermenticus SCD, a newly identified bacteriocin of Bacillus polyfermenticus. Left. Appl. Microbiol. 32, 146-151
  23. McCarthy, T. L., Kerry, J. P., Kerry, J. F., Lynch, P. B., and Buckley, D. J. (2001) Evaluation of the antioxidant potential of natural food plant extracts as compared with synthetic antioxidants and vitamin E in raw and cooked pork patties. Meat Sci. 57, 45-52
  24. McCune, L. M. and Johne, T. (2002) Antioxidant activity in medicinal plants associated with the symptoms of diabetes mellitus used by the indigenous peoples of the north american boreal forest. J. Ethnopharmacol. 82, 197-205 https://doi.org/10.1016/S0378-8741(02)00180-0
  25. Mitosumoto, M., Cassens, R. G., Schaefer, D. M., and Scheller, K. K. (1991) Pigment stability improvement in beef steaks by ascorbic acid application. J. Food Sci. 56, 857-858 https://doi.org/10.1111/j.1365-2621.1991.tb05399.x
  26. Naveena, B. M., Muthukumar, M., Sen, A. R., Babji, Y., and Murthy, T. R. K. (2006) Improvement of shelf-life of buffalo meat using lactic acid, clove oil and vitamin C during retail display. Meat Sci. 74, 409-415 https://doi.org/10.1016/j.meatsci.2006.04.020
  27. Paik, H. D., Park, J. S., and Park, E. (2005) Effects of Bacillus polyfermenticus SCD on lipid and antioxidant metabolisms in rats fed and high-cholesterol diet. BioI. Pharm. Bull. 28. 1270-1274 https://doi.org/10.1248/bpb.28.1270
  28. Park, J. G., Her, J. H., Li, S. Y., Cho, S. H., Youn, S. K., Choi, J. S., Park, S. M., and Ahn, D. H. (2005) Study on the improvement of storage property and quality in the traditional seasoning beef containing medicinal herb extracts. Kor. J. Soc. Food Sci. Nutr. 34, 113-119 https://doi.org/10.3746/jkfn.2005.34.1.113
  29. Reid, G., Sanders, M. E., Gaskins, H. R., Gibson, G. R., Mercenier, A, Rastall, R., Roberfroid, M., Rowland, I., Cherbut, C., and Klaenhammer, T. R. (2003) New scientific paradigms for probiotics and prebiotics. J. Clin. Gastroenterol. 37, 105-118 https://doi.org/10.1097/00004836-200308000-00004
  30. Romond, M. B., Yazourh, A., and Romond, C. (1997) Cell free wheys from bifidobacteria fermented milks exert a regulatory effect on intestinal microflora mice and humans. Anaerobe 3, 137-143 https://doi.org/10.1006/anae.1997.0090
  31. Rossel, J. B. (1994) Measurements of rancidity. In: Rancidity in foods, 3rd ed, Allen, J. C. (ed.), and Hamilton, R. J., pp. 22-53
  32. SAS. (1999) SAS/STAT Software for PC. SAS Institute Inc., Cary, NC, USA
  33. Shahid, F. and Wanasundara, P. K. J. P. D. (1992) Phenolic antioxidants. Crit. Rev. Food Sci. Nutr. 32, 67-103 https://doi.org/10.1080/10408399209527581
  34. Shahidi, F., Rubin, L. J., and Wood, D. F. (1987) Control of lipid oxidation in cooked ground pork with antioxidants and dinitrosyl ferrohemochrome. J. Food Sci. 52, 564-567 https://doi.org/10.1111/j.1365-2621.1987.tb06675.x
  35. Tarladgis, B. G., Watts, B. M., Younathan, M. T., and Dugan, L. R. (1960) A distillation method for the quantitative determination of malonaldehyde in rancid foods. J. Am. Oil Chemists Soc. 37, 44-47 https://doi.org/10.1007/BF02630824
  36. Tichivangana, J. Z. and Morrissey, P. A (1985) Metmyoglobin and inorganic metals as prooxidants in raw and cooked muscle system. Meat Sci. 15, 107-116 https://doi.org/10.1016/0309-1740(85)90051-8
  37. Yanishlieva, N. V. and Marinova, E. M. (2001) Stabilisation of edible oils with natural antioxidants. European J. Lipid Sci. Technol. 103, 752-767 https://doi.org/10.1002/1438-9312(200111)103:11<752::AID-EJLT752>3.0.CO;2-0
  38. Zandi, P. and Gondon, M. H. (1999) Antioxidant activity of extracts from old tea leaves. Food Chem. 64, 285-288 https://doi.org/10.1016/S0308-8146(98)00047-8
  39. 高坂和久 (1975) 肉製品の鮮度保持と測定. 食品工業 18, 105-111