Reproductive and Developmental Biology

The Korean Society of Animal Reproduction (한국동물번식학회)
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Expression Patterns of $TGF-{\beta}1,\;TGF-{\beta}$ Receptor Type I, II and Substrate Proteins Smad 2, 3, 4 and 7 in Bovine Oocytes and Embryos

  • Chung, Hak-Jae (Molecular Endocrinology Laboratory, Department of Animal Biotechnology, National Livestock Research Institute, RDA) ;
  • Kim, Bong-Ki (Molecular Endocrinology Laboratory, Department of Animal Biotechnology, National Livestock Research Institute, RDA) ;
  • Kim, Jong-Mu (Department of Animal Science, Chungbuk National University) ;
  • Lee, Hyun-Gi (Molecular Endocrinology Laboratory, Department of Animal Biotechnology, National Livestock Research Institute, RDA) ;
  • Han, Joo-Hee (Molecular Endocrinology Laboratory, Department of Animal Biotechnology, National Livestock Research Institute, RDA) ;
  • Kim, Nam-Hyung (Department of Animal Science, Chungbuk National University) ;
  • Park, Jin-Ki (Molecular Endocrinology Laboratory, Department of Animal Biotechnology, National Livestock Research Institute, RDA) ;
  • Seong, Hwan-Hoo (Molecular Endocrinology Laboratory, Department of Animal Biotechnology, National Livestock Research Institute, RDA) ;
  • Yang, Boh-Suk (Molecular Endocrinology Laboratory, Department of Animal Biotechnology, National Livestock Research Institute, RDA) ;
  • Chang, Won-Kyong (Molecular Endocrinology Laboratory, Department of Animal Biotechnology, National Livestock Research Institute, RDA) ;
  • Ko, Yeoung-Gyu (Molecular Endocrinology Laboratory, Department of Animal Biotechnology, National Livestock Research Institute, RDA)
  • Published : 2006.12.31
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Abstract

Transforming growth $factor-{\beta}\;(TGF-{\beta})$ has been shown to have a positive effect on in vitro fertilization (IVF) and has been reported to stimulate meiosis at follicular level in variety of species. The study was designed to determine the expression patterns of $TGF-{\beta}1,\;TGF-{\beta}$ receptors type I, II and Smads gene in bovine oocytes and embryos. $TGF-{\beta}1$ and their receptors were observed in the unfertilized oocytes. $TGF-{\beta}1$ and type II receptor were not expressed at the blastocyst stage, however, only type I receptor was exclusively observed at the same stage. The blastocyst stage, in particular, showed high levels of mRNA expression patterns containing a $TGF-{\beta}1$ type I receptor. The mRNA expression pattern of Smad 2 at all stages of embryonic development was similar in all respect with $TGF-{\beta}1$ type I receptor. On the contrary, Smad 3 and 4 were expressed with high and low level mRNA at the blastocyst stage. In conclusion. it is suggested that $TGF-{\beta}1$ signaling may be regarded as an important entity during the preimplantation embryo development.

Keywords

References

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