Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
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Anti-oxidant Activities and Anti-inflammatory Effects on Artemisia scoparia

비쑥 추출물에 대한 항산화 활성 및 염증억제 효과

  • Yoon, Weon-Jong (Jeju Biodiversity Research Institute, Jeju Hi- Tech Industry Development Institute) ;
  • Lee, Jung-A (Jeju Biodiversity Research Institute, Jeju Hi- Tech Industry Development Institute) ;
  • Kim, Ji-Young (Jeju Biodiversity Research Institute, Jeju Hi- Tech Industry Development Institute) ;
  • Oh, Dae-Ju (Jeju Biodiversity Research Institute, Jeju Hi- Tech Industry Development Institute) ;
  • Jung, Yong-Hwan (Jeju Biodiversity Research Institute, Jeju Hi- Tech Industry Development Institute) ;
  • Lee, Wook-Jae (Jeju Biodiversity Research Institute, Jeju Hi- Tech Industry Development Institute) ;
  • Park, Soo-Yeong (Jeju Biodiversity Research Institute, Jeju Hi- Tech Industry Development Institute)
  • 윤원종 ((재)제주하이테크산업진흥원 제주생물종다양성연구소) ;
  • 이정아 ((재)제주하이테크산업진흥원 제주생물종다양성연구소) ;
  • 김지영 ((재)제주하이테크산업진흥원 제주생물종다양성연구소) ;
  • 오대주 ((재)제주하이테크산업진흥원 제주생물종다양성연구소) ;
  • 정용환 ((재)제주하이테크산업진흥원 제주생물종다양성연구소) ;
  • 이욱재 ((재)제주하이테크산업진흥원 제주생물종다양성연구소) ;
  • 박수영 ((재)제주하이테크산업진흥원 제주생물종다양성연구소)
  • Published : 2006.12.30
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Abstract

We investigated the antioxidant activities such as DPPH radical scavenging capacity, xanthine oxidase inhibitory activity, and superoxide radical scavenging capacity of the aqueous EtOH extract and its solvent fractions of Artemisia scoparia. The ethyl acetate fraction showed high antioxidant activity, compared to positive controls such as ascorbic acid, butylated hydroxy anisole (BHA), trolox, and allopurinol in these assay systems. Moreover, we examined the inhibitory effect of solvent fractions of A. scoparia on the production of pro-inflammatory factors that the nitric oxide (NO), inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2) and prostaglandin E2 $(PGE_2)$ production activated with LPS $(1{\mu}g/ml)$ in murine macrophage cell line RAW264.7. The amounts of protein levels were determined by immunoblottting. Tn the sequential fractions of hexane and dichloromethane inhibited the NO and $PGE_2$ production and the protein level of iNOS and COX-2. These results suggest that A. scoparia may have anti-inflammatory activity through the antioxidant activity and inhibition of pro-inflammatory factors.

Keywords

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