Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Effects of a Herbal Composition (HemoHIM) on the Activation of Dendritic Cells

생약복합조성물(HemoHIM)의 수지상세포 활성화 효과

  • Shin, Sung-Hae (Dept. of Biology, College of Natural Science, Sunchon National University) ;
  • Kim, Do-Soon (Dept. of Biology, College of Natural Science, Sunchon National University) ;
  • Kim, Sung-Ho (College of Veterinary Medicine, Chonnam National University) ;
  • Jo, Sung-Kee (Radiation Food and Biotechnology Team, Advanced Radiation Technology Institute, Jeongeup Campus of Korea Atomic Energy Research Institute) ;
  • Byun, Mung-Woo (Radiation Food and Biotechnology Team, Advanced Radiation Technology Institute, Jeongeup Campus of Korea Atomic Energy Research Institute) ;
  • Yee, Sung-Tae (Dept. of Biology, College of Natural Science, Sunchon National University)
  • 신성해 (순천대학교 생물학과) ;
  • 김도순 (순천대학교 생물학과) ;
  • 김성호 (전남대학교 수의학과) ;
  • 조성기 (한국원자력연구소 방사성연구원 방사선식품생명공학팀) ;
  • 변명우 (한국원자력연구소 방사성연구원 방사선식품생명공학팀) ;
  • 이성태 (순천대학교 생물학과)
  • Published : 2006.12.29
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Abstract

In our previous study, a novel herb mixture (HIM-I) of Angelica gigas radix, Cnidium officinale rhizoma, and Paeonia japonica radix was developed to protect= the intestinal and immune systems and to promote their recovery from radiation damage. A new herbal composition (HemoHIM) with the high immune modulating activity was developed from HIM-I. In the present study, we examined the effects of HemoHIM on the maturation process of murine bone marrow (BM)-derived dendritic cells (DC). BM cells were cultured in the presence of iL-4 and GM-CSF and the generated immature DC were stimulated with HemoHIM for 24 hours. HemoHIM significantly enhanced the expression of co-stimulatory molecules, CD80 and CD86, especially. The activation capacity of HemoHIM-treated DC was significantly higher than that of immature DC, as analyzed by IL-2 and $IFN-\gamma$ production and proliferation of the responding T cells in the co-culture with allogeneic T cells. The antigen-presenting capacity of HemoHIN-treated DC was also increased by the co-culture with OVA-specific T cells (HS-1), as analyzed by IL-2 and $IFN-\gamma$ production and the proliferation. These results indicate that HemoHIM causes the maturation and ;Activation of DC, which may be a part of mechanisms of immunomodulation by HemoHIM.

특이적 면역반응을 유도하는 전문 항원제시세포인 수지상세포의 분화와 항원제시기능에 미치는 HemoHIM의 면역기능 증강 효과에 대해 알아보았다. HemoHIM은 수지상세포의 분화/증식 과정에 첨가하였을 경우에 공동자극 분자인 CD40, CD86 분자의 세포 표면 발현량을 증가시키는 것으로 나타났다. 이 수지상세포는 동종항원 반응 T세포를 활성화시켜 IL-2, $IFN-\gamma$ 분비량과 증식반응을 유의하게 증가시켰다. 그리고 HemoHIM을 처리한 수지상세포는 대조군에 비해 OVA항원을 T세포주에 충분히 전달하여 IL-2와 $IFN-\gamma$의 분비량을 유의하게 증가시켰을 뿐만 아니라 T세포주의 증식반응도 유의하게 증가시켰다. 따라서 HemoHIM은 생체 내의 특이적 면역반응을 유도하는 수지상세포의 기능을 강화하는 면역기능 조절제로서 사용이 가능할 것으로 생각된다.

Keywords

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