New Approach to the Analysis of Palindromic Structure in Genome Sequences

  • 발행 : 2006.12.31

초록

PABAP (Palindrome Analysis by BLAST Program) is an analysis system that identifies palindromic sequences from a large genome sequence up to several megabases long. It uses NCBI BLAST as a searching engine, and data processing such as alignment filtration and detection of inverted repeats which satisfy user-defined parameters is performed by manipulating data after populating into a MySQL database. PABAP outperforms publicly available palindrome search program in that it can detect large palindrome with internal spacer at a faster speed from bacterial genomes. It is a standalone application and is freely available for noncommercial users.

키워드

참고문헌

  1. Altschul, S.F., W. Gish, W. Miller, E.W. Myers, and D.J. Lipman. (1990). Basic local alignment search tool. J. Mol. Biol. 215, 403-410 https://doi.org/10.1016/S0022-2836(05)80360-2
  2. Chen, Y. and G.N. Gill. (1996). A heterodimeric nuclear protein complex binds two palindromic sequences in the proximal enhancer of the human erbB-2 gene. J. Biol. Chem. 271, 5183-5188 https://doi.org/10.1074/jbc.271.9.5183
  3. Cuomo, C.A., Mundy, C.L., and Oettinger, M.A. (1996). DNA sequence and structure requirements for cleavage of V(D)J recombination signal sequences. Mol. Cell Biol. 16, 5683-5690 https://doi.org/10.1128/MCB.16.10.5683
  4. Gelfand, M.S. and E.V. Koonin. (1997). Avoidance of palindromic words in bacteriol and archaeal genomes: a close connection with restriction enzymes. Nucleic Acids Res. 25, 2430-2439 https://doi.org/10.1093/nar/25.12.2430
  5. Mori, K., N. Ogawa, T. Kawahara, H. Yanagi, and Yura, T. (1998). Palindrome with spacer of one nucleotide is characteristic ofthe cis-acting unfolded protein response element in Saccharomyces cerevisiae. J. Biol. Chem. 273, 9912-9920 https://doi.org/10.1074/jbc.273.16.9912
  6. Rocha, E.P., Danchin, A., and Viari, A. (2001). Evolutionary role of restriction/modification systems as revealed by comparative genome analysis. Genome Res. 11, 946-958 https://doi.org/10.1101/gr.GR-1531RR
  7. Tashiro, J., K. Kinoshita, and Honjo, T. (2001). Palindromic but not G-rich sequences are targets of class switch recombination. Int. Immunol. 13, 495-505 https://doi.org/10.1093/intimm/13.4.495