Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Effects of Pleurotus eryngii on Lipid Levels and Enzyme Activity in Male Rats Fed High Cholesterol Diets

콜레스테롤 식이를 섭취한 흰쥐에 새송이버섯이 지질대사 및 효소활성에 미치는 영향

  • 이충언 (신라대학교 생물과학과)
  • Published : 2006.12.01
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Abstract

Effects of dietary supplementation of Pleurotus eryngii(PE) to a diet containig the cholesterol on lipid levels in the liver and serum, and enzyme activities of rats were studied by feeding 8-weeks old male rats with the four diets for 4 weeks, respectively: normal diet, control diet (10% fat + 0.5% cholesterol), 3% and 5% PE diets (control diet + 3% and 5% PE dry powder). The body weight gains and food efficiency ratios of the 3% and 5% PE diet groups were significantly lower than those of the control group. The liver and epididymal fat pad weights, and hepatic triglyceride Levels of the 5% PE diet group were more significantly decreased than those of the control group. The concentrations in serum triglyceride, total cholesterol, LDL+VLDL-cholesterol, and atherogenic index ratios were significantly lower in the 3% or 5% PE groups compared to those of the control group. Ratio of HDL-cholesterol to total-cholesterol was significantly higher in the 3% and 5% PE groups compared to that of the control group. The fecal total lipid excretion of the 5% PE diet group was more significantly decreased than that of the control group. There was no significant difference in the hepatic cholesterol, and the serum HDL-cholesterol concentrations, and the GOT, GPT and ${\gamma}-GTP$ activities among the experimental groups. These results showed that the Pleurotus eryngii powder feeding decreased the total cholesterol, LDL-cholesterol and atherogenic index, and increased the ratio of HDL-cholesterol to total-cholesterol in serum of the rats.

새송이버섯이 고콜레스테롤 식이를 섭취한 흰쥐의 지질대사에 미치는 영향을 조사하고자, 생후 8주령 숫쥐를 정상식이군, 대조식이군(5% 옥수수기름, 5% 돈지 및 0.5% 콜레스테롤을 첨가한 식이) 및 대조식이에 새송이버섯 분말을 3% 및 5%씩 첨가한 식이군 (3% 및 5% 새송이버섯군) 등 4군으로 나누어 4주간 사육한 결과는 다음과 같다. 실험동물의 체중증가량 및 식이효율은 대조군에 비하여 3% 및 5% 새송이버섯군이 유의하게 감소되었다. 간과 부고환지방의 무게는 대조군에 비해 5% 새송이버섯군이 유의하게 감소되었다. 간의 콜레스테롤 및 중성지질 농도는 대조군에 비하여 5% 새송이버섯군이 유의하게 감소되었다. 혈청의 중성지 질, 총 콜레스테롤과 LDL+VLDL-콜레스테롤 농도 및 동맥경화지수는 대조군에 비해 새송이버섯군들이 유의하게 감소되었다. 총 콜레스테롤에 대한 HDL-콜레스테롤의 비율은 대조군에 비해 새송이버섯군들이 유의하게 증가되었다. 변의 지질배설량은 5% 새송이버섯군이 대조군 보다 유의하게 증가된 것으로 나타났다 이상의 결과로 보아 고콜레스테롤 식이에 새송이버섯 분말 첨가 급여시 흰쥐의 일부 지질대사를 개선시키는 것으로 나타났다.

Keywords

References

  1. Anderson, J. W. and C. A. Bryant. 1986. Dietary fiber: Diabetes and obesity. Am. J. Gastroenterol 81, 898-905
  2. AOAC. 1980. Official of analysis. pp. 211-260, 15th eds., Association of Official Analytical Chemists. Washington DC
  3. Bobek, P., L. Ozdin and L. Kuniak. 1997. Effect of oyster mushroom and isolated ${\beta}$-glucan on lipid peroxidation and on the activities of antioxidative enzymes in rats fed the cholesterol diet. J. Nutr. Biochem. 8, 469-489 https://doi.org/10.1016/S0955-2863(97)00058-2
  4. Bobek, P., L. Ozdin and S. Galbavy. 1998. Dose- and time-dependent hypocholesterolemic effect of oyster mushroom (Pleurotus ostreatus) in rats. Nutrition 14, 282-286 https://doi.org/10.1016/S0899-9007(97)00471-1
  5. Cheung, P. C. K. 1996. The hyporcholesterolemic effect of extracellular polysaccharide from the submerged fermentation of mushroom. Nutr. Res. 16, 1953-1957 https://doi.org/10.1016/S0271-5317(96)00218-7
  6. Cheung, P. C. K. 1996. The hyporcholesterolemic effect of two edible mushrooms: Auricularia aurivula (tree-ear) and Tremella fuciformis (white jelly-leaf) in hypercholesterolemic rats. Nutr. Res. 16, 1721-1725 https://doi.org/10.1016/0271-5317(96)00191-1
  7. Cheung, P. C. K. 1998. Plasma and hepatic cholesterol levels and fecal neutral sterol excretion are altered in hamsters fed straw mushroom diets. J. Nutr. 128, 1512-1516 https://doi.org/10.1093/jn/128.9.1512
  8. Chibada, I., K. Okumura, S. Takeyama and A. Kotera. 1969. Lentinacin, a new hypocholesterolemic substance in Lentinus edodes. Experientia 25, 1237-1242 https://doi.org/10.1007/BF01897467
  9. Ebihara, K. and B. O. Schneeman. 1989. Interaction of bile acids, phospholipids, cholesterol and triglycerides with dietary fiber in the small intestine of rats. J. Nutr. 119, 1100-1106 https://doi.org/10.1093/jn/119.8.1100
  10. Folch, J., M. Lees and G. S. H. Stanley. 1957. A simple method for the isolation and purification of total lipid from animal tissues. J. Biol. Chem. 226, 497-509
  11. Frings, C. S. and R. T. Dunn. 1970. A colorimetric method for determination of total serum lipid based on the sulfophospho-vanillin reaction. Am. J. Clin. Path. 53, 89-91 https://doi.org/10.1093/ajcp/53.1.89
  12. Guillen F., C. Munoz, V. Gomez-Toribio, A. T. Martinez and, M. J. Martinez. 2000. Oxygen activation during oxidation of methoxyhydroquinones by laccase from Pleurotus eryngii. Applied Enviorn. Microbiology 66, 170-175 https://doi.org/10.1128/AEM.66.1.170-175.2000
  13. Haglund, O., R. Loustarinen, R. Wallin, I. Wibell and T. Saldeen. 1991. The effect of fish oil on triglycerides, cholesterol, fibrinogen and malondialdehyde in humans supplemented with vitamin. Eur. J. Nutr. 121, 165-172 https://doi.org/10.1093/jn/121.2.165
  14. Hwang, Y. J., H. K. Nam and S. H. Kim. 2003. Effeect of Lentinus edodes and Pleurotus eryngii. extracts on proliferation and apoptosis in Human colon cancer cell lines. J. Korean Soc. Food Sci. Nutr. 31, 217-222 https://doi.org/10.3746/jkfn.2003.32.2.217
  15. Kang, T. S., M. S. Kang and S. Y. Lee. 2001. Effect of Pleurotus eryngii. on the blood glucose and cholesterol in diabetic rats. Korean J. Mycology 29, 86-90
  16. Kailash, P. 1999. Reduction of serum cholesterol and hypercholesterolemic atherosclerosis in rabbits by secoisolariciresinol diglucoside isolated from flaxseed. Circulation 99, 1355-1362 https://doi.org/10.1161/01.CIR.99.10.1355
  17. Kim, J. Y., H. I. Kang and K. I. Seo. 2004. Antioxidative and antitumor activities of crude polysaccaride fraction Pleurotus eryngii. J. Korean Soc. Food Sci. Nutr. 33, 1589-1593 https://doi.org/10.3746/jkfn.2004.33.10.1589
  18. Koh, J. B. and M. A. Choi. 2001. Effect of Cordyceps militaris on lipid metabolism in rats fed cholesterol diet. Korean J. Nutrition 34, 265-270
  19. Koh, J. B. 2002. Effect of Cordyceps militaris on lipid metabolism, protein levels and enzyme activities in rats fed high fat diet. Korean J. Nutrition 35, 414-420
  20. Koh, J. B. 2003. Effect of liquid cultures of Cordyceps militaris on lipid metabolism and enzyme activities in hyperlipidemic female rats. Korean Journal of Life Science 13, 265-272 https://doi.org/10.5352/JLS.2003.13.3.265
  21. Koh, J. B. and M. A. Choi. 2003. Effect of Paecilomyes japonica on lipid metabolism in rats fed high fat diet. J. Korean Soc. Food Sci. Nutr. 32, 238-243 https://doi.org/10.3746/jkfn.2003.32.2.238
  22. Koh, J. B. and J. Y. Kim. 2004. Effects of liquid culture of Agaricus blazei Murill on lipid metabolism in rats fed cholesterol diet. Journal of Life Science 14, 531-536 https://doi.org/10.5352/JLS.2004.14.4.531
  23. Koh, J. B. 2005. Effects of Pleurotus eryngii on lipid metabolism in rats fed high fat diet. J. Korean Soc. Food Sci. Nutr. 34, 626-631 https://doi.org/10.3746/jkfn.2005.34.5.626
  24. Koh, J. B. 2005. Effects of liquid culture of Coriolus versicolor on lipid metabolism and enzyme activities in rats fed cholesterol diet. Journal of Life Science 15, 790-795 https://doi.org/10.5352/JLS.2005.15.5.790
  25. Kubo, K. and N. Nanba. 1996. The effect of Maitake mushrooms on liver and serum lipids. Altern. Ther. Health Med. 2, 62-69
  26. Liu, F., V.E. Ooi and S. T. Chang. 1997. Free radical scanvenging activities of mushroom polysaccharide extracts. Life Sci. 60, 763-766 https://doi.org/10.1016/S0024-3205(97)00004-0
  27. Manzi, P., L. Gambelli, S. Marconi, V. Vivanti and L. Pizzoferrato. 1999. Nutrients in edible mushrooms: an inter-species comparative study. Food Chemistry 65, 477-482 https://doi.org/10.1016/S0308-8146(98)00212-X
  28. Manzi, P., S. Marconi, A. Aguzzi and L. Pizzoferrato. 2004. Commercial mushrooms: nutritional quality and effect of cooking. Food Chemistry 84, 201-206 https://doi.org/10.1016/S0308-8146(03)00202-4
  29. Menoli, R. C. R. N., M. S. Mantovani, L. R. Ribeiro, G. Speit and B. Q. Jordao. 2001. Antimutagenic effects of the mushroom Agaricus blazei Murrill extracts on V79 cells. Mutation Research 496, 5-13 https://doi.org/10.1016/S1383-5718(01)00227-3
  30. Moon, S. P. and J. B. Koh. 2004. Effects of liquid culture of Coriolus versicolor on lipid metabolism and enzyme activities in rats fed high fat diet. Korean J. Nutrition 37, 88-94
  31. Oh, S. W., C. U. Lee and J. B. Koh. 2004. Effects of Agaricus blazei Murill on lipid metabolism in rats fed high fat diet. J. Korean Soc. Food Sci. Nutr. 33, 821-826 https://doi.org/10.3746/jkfn.2004.33.5.821
  32. Reeves, P. G., F. H. Nielsen and G. C. Fahey. 1993. AIN-93 purified diets for laboratory rodents final report of the American Institute of Nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet. J. Nutr. 123, 1939-1951 https://doi.org/10.1093/jn/123.11.1939
  33. Sugiyama, K., S. Saeki and Y. Ishiguro. 1992. Hypercholesterolemic activity of ningyotake (Poyporus confluens) mushroom in rats. J. Jpn. Soc. Nutr. Food Sci. 45, 265-270 https://doi.org/10.4327/jsnfs.45.265
  34. Sugiyama, K., T. Akachi and A. Yamakawa. 1995. Hypocholesterolemic action of eritadenine is mediate by a modification of hepatic phospholipid metabolism in rat. J. Nutr. 125, 2134-2140 https://doi.org/10.1093/jn/125.8.2134
  35. Tsang, K. W., C. L. Lam and W. K. Lam. 2003. Coriolus versicolor polysaccharide peptide slows progression of advanced non-small cell lung cancer. Respiratory Medicine 97, 618-624 https://doi.org/10.1053/rmed.2003.1490
  36. Yoshiaki, F., K. Hidekazu, O. Koichi, S. Ryo and E. Takusaburo. 1998. Tumoricidal activity of high molecular weight polysaccharides derived from Agaricus blazei via oral administration in the mouse tumor model. J. Jpn. Soc. Nutr. Food Sci. 45, 246-252 https://doi.org/10.3136/nskkk.45.246

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