Parasites, Hosts and Diseases

The Korea Society for Parasitology and Tropical Medicine (대한기생충학·열대의학회)
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Hydrogenosomal activity of Trichomonas varinalis cultivated under different iron conditions

  • Kim, Yong-Seok (Department of Biochemistry and Molecular Biology, Hanyang University College of Medicine) ;
  • Song, Hyun-Ouk (Department of Parasitology, The Brain Korea 21 Project, Hanyang University College of Medicine) ;
  • Choi, Ik-Hwa (Department of Parasitology, The Brain Korea 21 Project, Hanyang University College of Medicine) ;
  • Park, Soon-Jung (Department of Parasitology and Institute of Tropical Medicine, The Brain Korea 21 Project, Yonsei University College of Medicine) ;
  • Ryu, Jae-Sook (Department of Parasitology, The Brain Korea 21 Project, Hanyang University College of Medicine)
  • Published : 2006.12.10
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Abstract

To evaluate whether iron concentration in TYM medium influence on hydrogenosomal enzyme gene expression and hydrogenosomal membrane potential of Trichomonas vaginalis, trophozoites were cultivated in iron-depleted, normal and iron-supplemented TYM media. The mRNA of hydrogenosomal enzymes, such as pyruvate ferredoxin oxidoreductase (PFOR), hydrogenase, ferredoxin and malic enzyme, was increased with iron concentrations in T. vaginalis culture media, measured by RT-PCR. Hydrogenosomal membrane potentials measured with $DiOC_6$ also showed similar tendency, e.g. T. vaginalis cultivated in iron-depleted and iron-supplemented media for 3 days showed a significantly reduced and enhanced hydrogenosomal membrane potential compared with that of normal TYM media, respectively. Therefore, it is suggested that iron may regulate hydrogenosomal activity through hydrogenosomal enzyme expression and hydrogenosomal membrane potential.

Keywords

References

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