Parasites, Hosts and Diseases

The Korea Society for Parasitology and Tropical Medicine (대한기생충학·열대의학회)
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Molecular characterization of Acanthamoeba isolated from amebic keratitis related to orthokeratology lens overnight wear

  • Lee, Jun-Joo (Department of Parasitology, School of Medicine, Pusan National University) ;
  • Jeong, Hae-Jin (Department of Parasitology, School of Medicine, Pusan National University) ;
  • Lee, Ji-Eun (Department of Ophthalmology, School of Medicine, Pusan National University) ;
  • Lee, Jong-Soo (Department of Ophthalmology, School of Medicine, Pusan National University) ;
  • Xuan, Ying-Hua (Department of Parasitology, School of Medicine, Kyungpook National University) ;
  • Kong, Hyun-Hee (Department of Parasitology, School of Medicine, Kyungpook National University) ;
  • Chung, Dong-Il (Department of Parasitology, School of Medicine, Kyungpook National University) ;
  • Ock, Mee-Sun (Department of Parasitology, Kosin Medical College) ;
  • Yu, Hak-Sun (Department of Parasitology, School of Medicine, Pusan National University)
  • Published : 2006.12.10
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Abstract

In an effort to characterize, on the molecular scale, the Acanthamoeba initially isolated from the cornea of an amoebic keratitis patient associated with overnight-wear orthokeratology lens in Korea, we conducted mitochondrial DNA restriction fragment length polymorphism, 18S rDNA sequencing, and drug sensitivity analyses on the isolate (KA/PE1). The patient was treated with polyhexamethylene biguanide, chlorhexidine and oral itraconazole, which resulted in resolution of the patient's ocular inflammation. The majority of the molecular characteristics of the KA/PE1 were determined to be identical, or quite similar, to those of A. castellanii Ma strain, which had been isolated also from amoebic keratitis. The risk of Acanthamoeba keratitis as a potential complication of overnight orthokeratology is briefly discussed.

Keywords

References

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