Journal of Chest Surgery

The Korean Society for Thoracic and Cardiovascular Surgery (대한심장혈관흉부외과학회)
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TGF-${\beta}1$ Protein Expression in Bullae of Patients with Spontaneous Pneumothorax

자연기흉환자의 폐기포에서 TGF-${\beta}1$ 단백질 발현에 대한 연구

  • Kim, Kwang-Ho (Department of Thoracic and Cardiovascular Surgery, College of Medicine, Inha University) ;
  • Cho, Jung-Soo (Department of Thoracic and Cardiovascular Surgery, College of Medicine, Inha University) ;
  • Kim, Young-Sam (Department of Thoracic and Cardiovascular Surgery, College of Medicine, Inha University) ;
  • Yoon, Yong-Han (Department of Thoracic and Cardiovascular Surgery, College of Medicine, Inha University) ;
  • Kim, Joung-Taek (Department of Thoracic and Cardiovascular Surgery, College of Medicine, Inha University) ;
  • Baek, Wan-Ki (Department of Thoracic and Cardiovascular Surgery, College of Medicine, Inha University) ;
  • Kim, Lucia (Department of Pathology, College of Medicine, Inha University) ;
  • Song, Sun-U. (Clinical Research Center, College of Medicine, Inha University)
  • 김광호 (인하대학교 의과대학 흉부외과학교실) ;
  • 조정수 (인하대학교 의과대학 흉부외과학교실) ;
  • 김영삼 (인하대학교 의과대학 흉부외과학교실) ;
  • 윤용한 (인하대학교 의과대학 흉부외과학교실) ;
  • 김정택 (인하대학교 의과대학 흉부외과학교실) ;
  • 백완기 (인하대학교 의과대학 흉부외과학교실) ;
  • 김루시아 (인하대학교 의과대학 병리학교실) ;
  • 송순욱 (인하대학교 의과대학 임상연구센터)
  • Published : 2006.11.05
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Abstract

Background: In our previous study, we demonstrated that transforming growth factor-beta 1 receptor II(TGF-${\beta}1RII$) may have a role in the formation of bullae. In this study, we investigated if expression of transforming growth factor-beta 1 (TGF-${\beta}1$) ligand was altered in a bullous lung tissue by immunohistochemical staining of bullous tissues from patients with primary spontaneous pneumothorax. Material and Method: Bullous lung tissues were obtained from 36 patients with primary spontaneous pneumothorax, including 34 males and 2 females aged 14 to 38 years old. Result: Of the 36 patients, 19 were TGF-${\beta}1$ positive and 24 were transforming growth factor-beta 1 receptor II(TGF-${\beta}1RII$) positive. Among the 19 TGF-${\beta}1$ positives, 15 were also TGF-${\beta}1RII$ positive, observation at high magnification showed that strong immunohistochemical stain was detected in the boundary region between the bullous and normal lung tissues. Conclusion: These results suggest that overexpression of TGF-${\beta}1$ may be involved in the formation of a bulla as well as the alteration of TGF-${\beta}1RII$ expression. Further molecular studies are needed to elucidate the more detailed molecular mechanisms of the bulla formation.

배경: Transforming growth factor-beta 1 receptor II(TGF-${\beta}1RII$) 단백질의 과발현이 폐기포 조직의 형성에 관여할 수 있다는 연구 결과를 본 연구팀은 발표한 바 있다. 이와 관련하여 폐기포벽에서 발견되는 섬유화에 관계될 것으로 생각되는 Transforming growth factor-beta 1(TGF-${\beta}1$) ligand의 발현의 변화를 보기 위하여 자연기흉 환자에서 폐기포 절제슬로 획득한 폐 기포조직을 면역조직화학적 염색법으로 염색하여 관찰하려 하였다. 대상 및 방법: 36명의 자연기흉의 환자에서 비디오흉강경 폐기포절제술 시 획득된 폐기포조직을 획득하였다. 36명 환자 중 남자가 34명, 여자가 2명이었고 연령은 14세에서 38세였다. 획득된 폐기포조직을 면역조직화학적 염색방법으로 염색하였다. 결과: 36명 중 19예에서 TGF-${\beta}1$ 양성이었고 24예에서는 transforming growth factor-beta 1 receptor II(TGF-${\beta}1RII$)가 양성이었다. 19예의 TGF-${\beta}1$ 양성 예 중 15예에서 TGF-${\beta}1RII$에도 양성으로 관찰되었다. 이 같은 양성 변화는 폐기포조직과 정상 폐 조직과의 경계선에서 가장 강하게 관찰되었다. 결론: TGF-${\beta}1$의 과발현은 TGF-${\beta}1RII$ 발현의 변이 여부와 마찬가지로 폐기포벽의 섬유화를 초래하여 폐기포 형성에 영향을 미치는 것으로 생각되며 확실한 규명을 위하여 분자생물학적 및 기타 추가적인 연구가 더 필요할 것으로 생각된다.

Keywords

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