DOI QR코드

DOI QR Code

Isolation of an Algal Growth-enhancer Polysaccharide from the Chlorophyta Monostroma nitidum

  • Cho, Ji-Young (Department of Marine Biotechnology, Soonchunhyang University) ;
  • Luyen Hai Quoc (Department of Biotechnology, Pukyong National University) ;
  • Khan Mohammed N.A. (Department of Biotechnology, Pukyong National University) ;
  • Shin, Hyun-Woung (Department of Marine Biotechnology, Soonchunhyang University) ;
  • Park, Nam-Gyu (Department of Biotechnology, Pukyong National University) ;
  • Hong, Yong-Ki (Department of Biotechnology, Pukyong National University)
  • Published : 2006.09.30

Abstract

A micro algal growth-enhancing polysaccharide compound was isolated from the green alga Monostroma nitidum using water extraction, molecular fractionation, a DEAE-cellulose column, and fast protein liquid chromatography using a Superose-12 column. The yield of the compound from the seaweed powder was 8.3$\times$l0$^{-3}$%. At 2 mg/mL concentration, the polysaccharide enhanced Tetraselmis suecica cell growth in f/2 medium by approximately 160%.

Keywords

References

  1. Cho, J.Y, H.J. Jin, H.J. Lim, J.N.C. Whyte and Y.K. Hong. 1999. Growth activation of the microalga Isochrysis galbana by the aqueous extract of the seaweed Monostroma nitidum. J. Appl. Phycol., 10, 561-567 https://doi.org/10.1023/A:1008010519923
  2. Cho. J.Y., J.S. Choi, S.E. Kang, S.J. Ahn, J.K. Kim and Y.K. Hong. 2005. Growth enhancement of the microalga Tetraselmis suecica by an extract of the green alga Monostroma nitidum. J. Fish. Sci. Technol., 8, 1-5 https://doi.org/10.5657/fas.2005.8.1.001
  3. Dawson, R.M.C., D.C. Elliott, W.H. Elliott and K.M. Jones. 1986. Data for Biochemical Research. 3rd ed. Oxford University Press, NY, USA, 1-580
  4. Guillard, R.L. and J.H. Ryther. 1962. Studies of marine planktonic diatoms. 1. Cyclotella nana Hustedt and Detonule conferyacea (Cleve) Gran. Can. J. Microbiol., 8, 229-239 https://doi.org/10.1139/m62-029
  5. Lim, H.J. and S.R. Park. 1998. Changes in survival rate, nutritional value and fatty acid profile of concentrated food phytoplanktons by cold storage. J. Kor. Fish. Soc., 31, 567-573
  6. Lopez Alonso, D., E. Molina Grima, J.A. Sanchez Perez, J.L. Garcia Sanchez and F. Garcia Camacho. 1992. Isolation of clones of Isochrysis galbana rich in eicosapentaenoic acid. Aquaculture, 102, 363-371 https://doi.org/10.1016/0044-8486(92)90189-R
  7. Luyen, H.Q., J.Y Cho, H.W. Shin, N.G. Park and Y.K. Hong. 2006. Microalgal growth enhancement by levoglucosan isolated from the green seaweed Monostroma nitidum. J. Appl. Phycol., 18 (in press)
  8. Mansour, M.P., D.M.F. Frampton, P.D. Nichols, J.K. Volkman and S.I. Blackburn. 2005. Lipid and fatty acid yield of nine stationary-phase microalgae: applications and unusual $C_{24}-C_{28} $polyunsaturated fatty acids. J. Appl. Phycol., 17,287 -300 https://doi.org/10.1007/s10811-005-6625-x
  9. Polne-Fuller, M., H. Amano, T. Fusimura, Y.K. Hong, D.A. Coury, I. Sousa-Pinto and A. Gibor. 1993. Enhanced development and differentiation of protoplasts and spores of green and red seaweeds by a Pterocladia agar from New Zealand. Hydrobiologia, 260/261, 499-504 https://doi.org/10.1007/BF00049062
  10. Pulz, O. 2001. Photobioreactors: production systems for phototrophic microorganisms. Appl. Microbiol. Biotechnol., 57, 287-293 https://doi.org/10.1007/s002530100702
  11. Wikfors, G.H. and M. Ohno. 2001. Impact of algal research in aquaculture. J. Phycol., 37, 968-974 https://doi.org/10.1046/j.1529-8817.2001.01136.x