Reproductive and Developmental Biology

The Korean Society of Animal Reproduction (한국동물번식학회)
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Toxicologic Effects of BPA and DEHP on Semen Characteristics in Pig

BPA와 DEHP가 돼지 정액 성상에 미치는 독성 영향

  • Park, Dong-Heon (College of Animal Life Science, Kangwon National University) ;
  • Jang, Hyun-Yong (College of Animal Life Science, Kangwon National University) ;
  • Cheong, Hee-Tae (School of Veterinary Medicine, Kangwon National University) ;
  • Kim, Jong-Taek (School of Veterinary Medicine, Kangwon National University) ;
  • Park, Choon-Keun (College of Animal Life Science, Kangwon National University) ;
  • Kim, Choung-Ik (College of Animal Life Science, Kangwon National University) ;
  • Yang, Boo-Keun (College of Animal Life Science, Kangwon National University)
  • 박동헌 (강원대학교 동물생명과학대학) ;
  • 장현용 (강원대학교 동물생명과학대학) ;
  • 정희태 (강원대학교 수의학부대학) ;
  • 김종택 (강원대학교 수의학부대학) ;
  • 박춘근 (강원대학교 동물생명과학대학) ;
  • 김정익 (강원대학교 동물생명과학대학) ;
  • 양부근 (강원대학교 동물생명과학대학)
  • Published : 2006.09.30
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Abstract

The objective of this study was to investigate the toxic effects of Bisphenol A(BPA) and di-2 ethyhexyl phthalate (DEHP) as endocrine disrupters on sperm motility, viability, membrane integrity and abnormality during in vitro incubation of boar semen. Semen were randomly divide into 24 groups and healed with different concentrations of BPA md DEHP($1{\sim}100{\mu}M$) for 3, 6 and 9 hrs, respectively. The percentages of sperm motility and viability decreased by treatment time with both BPA and DEHP, and obiously differ from the controls. The percentages of sperm motility and viability significantly decreased by incubation with both $100{\mu}M$ of BPA and DEHP compared to control and other treatment groups(p<0.05). Sperm membrane integrity was significantly reduced by incubation with 10 and $100{\mu}M$ of BPA and DEHP, respectively(p<0.05), but sperm abnormality were not significantly affect both BPA and DEHP. These results indicate that high concentration of BPA and DEHP($>10{\mu}M$ can affect noxiously the sperm characteristics.

본 연구는 내분비계 장애물질인 BPA와 DEHP가 돼지 정액 성상(운동성, 생존율, 원형질막의 정상성, 기형율)에 유해한 영향을 미치는지를 검토하였다. 일정 농도로 희석한 돼지 정액에 BPA와 DEHP를 각각 0, 1, 10, $100{\mu}M$의 농도로 처리하여 3, 6, 9시간 동안 체외배양을 실시하였다. BPA 처리에 따른 운동성과 생존율은 체외배양 시간과 첨가농도에 따라 감소하였으며, 체외배양시간이 경과함에 따라 대조구와 명백한 차이를 나타냈다. $100{\mu}M$의 농도로 처리한 경우의 운동성과 생존율은 체외배양시간에 관계없이 처리군이 대조구에 비해 유의적으로 감소하였다(p<0.05). DEHP처리에 따른 정자의 운동성과 생존율도 체외배양시간과 첨가농도에 따라 감소하여 BPA의 성적과 유사한 경향을 나타냈다. 정자 원형질막의 기능성은 배양시간에 따라 감소하였으며 BPA및 DEHP 첨가농도 간에 차이가 인정되었으며, 특히 $100{\mu}M$ 처리구에서 급격히 감소하는 성적을 나타났다. 정자의 기형율은 BPA및 DEHP 처리시간 및 농도에 크게 영향을 받지는 않았다. 본 연구의 결과로 볼 때, 고농도의 BPA와 DEHP($>10{\mu}M$에 정자의 장시간 노출은 유해한 영향을 줄 것으로 사료된다.

Keywords

References

  1. Agarwal DK, Eustis S, Lamb JC, Real JR, Kluwe WM (1986): Effects of di(2-ethylhexyl)phthalate on the gonadal pathophysiology, sperm morphology and reproductive performance of male rats. Environ Health Perspect 65:343-350 https://doi.org/10.2307/3430202
  2. Ashby B, Tinwell H, Haseman J (1999): Lack of effects for low dose levels of bisphenol A and diethylstilbestrol in the prostate gland of CF1 mice exposed in utero. Regula Toxicol Pharma 30:156-166 https://doi.org/10.1006/rtph.1999.1317
  3. Buchanan DL, Setiawan T, Lubahn DB, Taylor JA, Kurita T, Cunha GR, Cooke PS (1999): Tissue compartment-specific estrogen receptor-a participation in the mouse uterine epithelial secretory response. Endocrinol 140:484-491 https://doi.org/10.1210/en.140.1.484
  4. Busser MT, Lutz WK (1987): Stimulation of DNA synthesis in rat and mouse liver by various tumor promotors. Carcinogen 8:1433-1437 https://doi.org/10.1093/carcin/8.10.1433
  5. Cagen SZ, Waechoter JM, Dimond SS, Breslin WJ, Butala JH, Jekat FW, Shiotsuka RL, Veenstra GE, Harris, LR (1999): Normal reproductive organ development in CF-1 mice following prenatal exposure to bisphenol A. Toxico Sci 50:36-44 https://doi.org/10.1093/toxsci/50.1.36
  6. Colborn T, vom Seal FS, Soto AM (1993): Development effects of endocrine-disrupting chemicals in wildlife and human. Environ Health Perspect 101: 378-384 https://doi.org/10.2307/3431890
  7. Dodds EC, Lawson W (1936): Synthetic oestrogenic agents without the phenanthrene nucleus. Nature 137:996
  8. Gaido KW, Leonard LS, Gould JC, Babai D, Portier CJ, McDonnell DP (1997): Evaluation of chemicals with endocrine modulating activity in a yeast-based steroid hormone receptor gene transcription assay. Toxicol Appl Pharmacol 143:205-212 https://doi.org/10.1006/taap.1996.8069
  9. Grays TJB, Butter Worth KR, Gaunt IF, Grasso P, Gangolli SD (1977): Short-term toxicity study of di-(2-ethylhexyl)phthalate in rats. Food Cosmet Toxicol 15:389-399 https://doi.org/10.1016/S0015-6264(77)80003-5
  10. Hunter DS, Hodges LC, Vonier PM, Fuchs-Young R, Gottardis MM, Walker CL (1999): Estrogen receptor activation via activation function 2 predicts agonism of xenoestrogen in normal and neoplastic cells of the uterine myometrium. Cancer Res 59: 3090-3099
  11. Keys DA, DC Wallace, TB Kepler, RB Conolly (1999):Quantitative evaluation of alternative mechanism of blood and testes disposition of di(2-etylhexyl) phthalate and mono(2-etylhexyl)phthalate in rats. Toxicol Sci 49:172-185 https://doi.org/10.1093/toxsci/49.2.172
  12. Lazer NR (1995): Polycarbonate : high-performance resin. Adv Material Processes 147:43-45
  13. Oishi S, Hiraga K (1980): Testicular atrophy induced by phthalic acid esters : Effect on testosterone and zinc concentrations. Toxicol Appl Pharmacol 53: 35-41 https://doi.org/10.1016/0041-008X(80)90378-6
  14. Parmar D, Srivastava SP, Singh GB, Seth PK (1987): Effect of testosterone on the testicular atrophy caused by di(2-ehtylhexyl)phthalate. Toxicol Lett 36:297-308 https://doi.org/10.1016/0378-4274(87)90199-8
  15. Sharpe RM, Majdic G, Fisher J, Parte P, Miller MR, Saunders PTK (1996): Effects on testicular development and function. Int Cong Endocrinol pp:23-24
  16. Shiota K, Mima S (1985): Assessment of the teratogenicity of di(2-etylhexyl)phthalate and mono(2-etylhexyl)phthalate in mice. Arch Toxicol 56:263-266 https://doi.org/10.1007/BF00295165
  17. Staples CA, Dorn PB, Klecka GM, O'Block ST, Harris LR (1998): A review of the environmental fate : effects and exposures of bisphenol A. Chemosphere 36:2149-2173 https://doi.org/10.1016/S0045-6535(97)10133-3
  18. Steinmetz R, Mitchner NA, Grant A, Allen DL, Bigsby RM, Ben-Jonathan N (1998): The xenoestrogen bisphenol A induces growth, differentiation and cfos gene expression in the female reproductive tract. Endocrinol 139:2741-2747 https://doi.org/10.1210/en.139.6.2741
  19. Stoker TE, Robinette CL, Brit BH, Law SC, Cooper RL (1999): Prepubertal exposure to compounds that increase prolactin secretion in the male rat : effect on the adult prostate. Biol Reprod 61:1636-1643 https://doi.org/10.1095/biolreprod61.6.1636
  20. Sumpter JP (1998): Xenoendocrine distrupters-environmental impacts. Toxicol Lett 102-103:337-342 https://doi.org/10.1016/S0378-4274(98)00328-2
  21. Takai Y, Tsutsumi O, Ikezuki Y, Hiroi H, Osuga Y, Momoeda M, Yano T, Taketani Y (2000): Estrogen receptor-mediated effects of a xenoestrigen, bisphenol A, on preimplantation mouse embryos. Biochem Biophy Res Commun 270:918-921 https://doi.org/10.1006/bbrc.2000.2548
  22. Tsutsui T, Watanabe E, Barrett JC (1993): Ability of peroxisome prolilerators to induce cell transformation, chromosome aberrations and peroxisome proliferation in watured Syrian hamsters embryo cell. Carcinogen 14:611-618 https://doi.org/10.1093/carcin/14.4.611
  23. Vom Saal FS, Cooke PS, Buchanan D, Palanza P, Thayer UA, NagaI SC, Parmigiani S, Welshons W (1998): A physiologically based approach to the study of bisphenol A and other estrogenic chemicals on the size of reproductive organs, daily sperm production and behavior. Toxicol Ind Health 14: 239-260 https://doi.org/10.1177/074823379801400115
  24. Woodward KN (1990): Phthalate esters, cystic kidney disease in animals and possible effects on human health: a review. Hum Exp Toxicol 9:397-401 https://doi.org/10.1177/096032719000900607