Toxicological Research

  • 제22권3호
  • /
  • Pages.267-273
  • /
  • 2006
  • /
  • 1976-8257(pISSN)
  • /
  • 2234-2753(eISSN)
한국독성학회 (Korean Society of Toxicology & Korea Environmental Mutagen Society)
권호 표지

Tungtungmadic Acid Isolated from Salicornia herbacea Suppresses the Progress of Carbon Tetrachloride-induced Hepatic Fibrosis in Mice

  • Chung, Young-Chul (Division of Food Science, Chinju International University) ;
  • Choi, Jae-Ho (Department of Pharmacy, College of Pharmacy and Research Center for Proteineous Materials, Chosun University) ;
  • Oh, Kyo-Nyeo (Department of Pharmacy, College of Pharmacy and Research Center for Proteineous Materials, Chosun University) ;
  • Chun, Hyo-Kon (Korea Research Institute of Bioscience and Biotechnology) ;
  • Jeong, Hye-Gwang (Department of Pharmacy, College of Pharmacy and Research Center for Proteineous Materials, Chosun University)
  • 발행 : 2006.09.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Tungtungmadic acid(3-caffeoyl, 4-dihydrocaffeoyl quinic acid: CDCQ) is a new chlorogenic acid derivative isolated from the Salicornia herbacea. The suppressive effects of CDCQ on the progress of acute carbon tetrachloride($CCl_4$)-induced hepatic fibrosis were investigated in mice. CDCQ significantly suppressed $CCl_4$-induced hepatic necrosis and inflammation, as determined by serum enzymatic activities of alanine and aspartate aminotransferase and serum TNF-$\alpha$ levels in a dose-dependent manner. In addition, increased hepatic lipid peroxidation and fibrosis after acute $CCl_4$ treatment were suppressed by the administration of CDCQ. CDCQ also significantly prevented the elevation of hepatic hydroxyproline and collagen content and ${\alpha}$-smooth muscle actin(${\alpha}$-SMA) expression in the liver of $CCl_4$-intoxicated mice. These results suggest that the suppressive effects of CDCQ against the acute $CCl_4$-induced hepatic fibrosis possibly related to its ability to block both hepatic inflammation and the activation of hepatic stellate cells.

키워드

참고문헌

  1. Bradford, M.M. (1976): A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72, 248-254 https://doi.org/10.1016/0003-2697(76)90527-3
  2. Brandsten, C., Lundmark, C., Christersson, C., Hammarstrom, L. and Wurtz, T. (1999): Expression of collagen alphal(I) mRNA variants during tooth and bone formation in the rat. Journal of Dental Research, 78, 11-19 https://doi.org/10.1177/00220345990780010101
  3. Brattin, W.J., Glende, E.A. Jr. and Recknagel, R.O. (1985): Pathological mechanisms in carbon tetrachloride hepatotoxicity. Free Radical Biology and Medicine, 1, 27-38 https://doi.org/10.1016/0748-5514(85)90026-1
  4. Brenner, D.A., Waterboer, T., Choi, S.K., Lindquist, J.N., Stefanovic, B., Burchardt, E., Yamauchi, M., Gillan, A. and Rippe, R.A. (2000): New aspects of hepatic fibrosis. Journal of Hepatology, 32, 32-38
  5. Casini, A., Cunningham, M., Rojkind, M., and Lieber, C.S. (1991): Acetaldehyde increases procollagen type I and fibronectin gene transcription in cultured rat fat-storing cells through a protein synthesis-dependent mechanism. Hepatology, 13, 758-765
  6. Chung, Y.C., Chun, K.H., Yang, J.Y., Kim, J.Y., Han, E.H., Kho, Y.H. and Jeong, H.G. (2005): Tungtungmadic acid, a novel antioxidant, from Salicornia herbacea. Archives of Pharmacal Research, 28, 1122-1126 https://doi.org/10.1007/BF02972972
  7. Fairhurst, S., Barber, D.J., Clark, B. and Horton, A.A. (1982): Studies on paracetamolinduced lipid peroxidation. Toxicology, 23, 249-259 https://doi.org/10.1016/0300-483X(82)90102-0
  8. Fort, J., Pilette, C., Veal, N., Oberti, F., Gallois, Y., Douay, O., Rosenbaum, J. and Cales, P. (1998): Effects of long-term administration of interferon-alpha in two models of liver fibrosis in rats. Journal of Hepatology, 29, 263-270 https://doi.org/10.1016/S0168-8278(98)80012-3
  9. Friedman, S.L. (2000): Molecular regulation of hepatic fibrosis. An integrated cellular response to tissue injury. Journal of Biological Chemistry, 275, 2247-2250 https://doi.org/10.1074/jbc.275.4.2247
  10. Friedman, S.L. (1993) The cellular basis of hepatic fibrosis: Mechanism and treatment strategies. New England Journal of Medicine, 328, 1828-1835 https://doi.org/10.1056/NEJM199306243282508
  11. Houglum, K., Venkataramani, A., Lyche, K. and Chojkier, M. (1997): A pilot study of the effects of D-a-tocopherol on hepatic stellate cell activation in chronic hepatitis C. Gastroenterology, 113, 1069-1073 https://doi.org/10.1053/gast.1997.v113.pm9322499
  12. Im, S.-A., Kim, G.-W. and Lee, C.-K. (2003): Immunomodulatory activity of Salicomia herbacea L. components. Natural Products Sciences, 9, 273-277
  13. Iredale, J.P., Benyon, R.C., Arthur, M.J., Ferris, W.F., Alcolado, R. and Winwood, P.J. (1996): Tissue inhibitor of metalloproteinase- 1 messenger RNA expression is enhanced relative to interstitial collagenase messenger RNA in experimental liver injury and fibrosis. Hepatology, 24, 176-84 https://doi.org/10.1002/hep.510240129
  14. Jamall, I.S., Finelli, V.N. and QueHee, S.S. (1981): A simple method to determine nanogram levels of 4-hydroxyproline in biological tissues. Analytical Biochemistry, 112, 70-75 https://doi.org/10.1016/0003-2697(81)90261-X
  15. Kawada, N., Seki, S., Inoue, M. and Kuroki, T. (1998): Effect of antioxidants, resveratrol, quercetin, and N-acetylcysteine, on the functions of cultured rat hepatic stellate cells and Kupffer cells, Hepatology, 27, 1265-1274 https://doi.org/10.1002/hep.510270512
  16. Kim, C.S. and Song, T.G. (1993): Ecological studies on the halophyte communities at western and southern coasts in Korea. Korean Journal of Ecology, 6, 167-176
  17. Kitts, D.D., Wijewickreme, A.N. and Hu, C. (2000): Antioxidant properties of a North American ginseng extract. Molecular and Cellular Biochemistry, 203, 1-10 https://doi.org/10.1023/A:1007078414639
  18. Knittel. T., Schuppan, D., Meyer zum Buschenfelde, K.H. and Ramadori, G. (1992) Differential expression of collagen types I, III, and IV by fat-storing (Ito) cells in vivo. Gastroenterology, 102, 1724-1735 https://doi.org/10.1016/0016-5085(92)91736-N
  19. Kristensen, D.B., Kawada, N., Imamura, K., Miyamoto, Y., Tateno, C., Seki, S., Kuroki, T. and Yoshizato, K. (2000): Proteome analysis of rat hepatic stellate cells. Hepatology, 32, 268-277 https://doi.org/10.1053/jhep.2000.9322
  20. Kunstle, G., Hentze, H., Germann, P.G., Tiegs, G., Meergans, T. and Wendel, A. (1999): Concanavalin A hepatotoxicity in mice: Tumor necrosis factor-mediated organ failure independent of caspase-3-like protease activation. Hepatology, 30, 1241-1251 https://doi.org/10.1002/hep.510300517
  21. Lee, K.S., Buck, M., Houglum, K. and Chojkier, M. (1995): Activation of hepatic stellate cells by TGFa and collagen type Its mediated by oxidative stress through c-myb expression. Journal of Clinical Investigation, 96, 2461-2468 https://doi.org/10.1172/JCI118304
  22. Lee, Y.S., Lee, H.S., Shin, K.H., Kim, B.-K. and Lee, S.H. (2004): Constituents of the Halophyte Salicornia herbacea. Archives of Pharmacal Research, 27, 1034-1036 https://doi.org/10.1007/BF02975427
  23. Li, D. and Friedman, S.L. (1999): Liver fibrosis and the role of hepatic stellate cells: new insights and prospects for therapy. Journal of Gastroenterology and Hepatology, 14, 618-633 https://doi.org/10.1046/j.1440-1746.1999.01928.x
  24. Nakatsukasa, H., Nagy, P., Evarts, R.P., Hsia, C.C., Marsden, E. and Thorgeirsson, S.S. (1990): Cellular distribution of transforming growth factor-beta 1 and procollagen types I, III, and IV transcripts in carbon tetrachlorideinduced rat liver fibrosis. Journal of Clinical Investigation, 85, 1833-1843 https://doi.org/10.1172/JCI114643
  25. Pinzani, M. and Gentilini, P. (1999): Biology of hepatic stellate cells and their possible relevance in the pathogenesis of portal hypertension in cirrhosis. Seminars in Liver Disease, 19, 397-410
  26. Ramadori, G., Veit, T., Schwogler, S., Dienes, H. P., Knittel, T., Rieder, H. and Meyer zum Buschenfelde, K.H. (1990): Expression of the gene of the alpha-smooth muscle actin isoform in rat liver and in rat fat-storing (ITO) cells. Virchows Archiv. B. Cell Pathology, 59, 349-357 https://doi.org/10.1007/BF02899424
  27. Shiba, M., Shimizu, I., Yasuda, M., Ii, K. and Ito, S. (1998) Expression of type I and type III collagens during the course of dimethylnitrosamine-induced hepatic fibrosis in rats. Liver, 18, 196-204 https://doi.org/10.1111/j.1600-0676.1998.tb00150.x
  28. Svegliati Baroni, G., D'Ambrosio, L., Ferretti, G., Casini, A., Di Sario, A., Salzano, R., Ridolfi, F., Saccomanno, S., Jezequel, A. and Benedetti, A. (1998) Fibrogenic effect of oxidative stress on rat hepatic stellate cells. Hepatology, 27, 720-726 https://doi.org/10.1002/hep.510270313
  29. Tsukamoto, H., Rippe, R., Niemela, O. and Lin, M. (1995) Roles of oxidative stress in activation of Kupffer and Ito cells in liver fibrogenesis. Journal of Gastroenterology and Hepatology, 10, S50-S53 https://doi.org/10.1111/j.1440-1746.1995.tb01798.x