Rahnella aquatilis Strain AY2000 Produces an Anti-Yeast Substance

  • Ryu, Eun-Ju (Department of Cosmetology, Han-Seo University) ;
  • Kim, Han-Woo (Department of Life Science and Biotechnology, Dong-Eui University) ;
  • Kim, Byung-Woo (Department of Life Science and Biotechnology, Dong-Eui University) ;
  • Kwon, Hyun-Ju (Department of Life Science and Biotechnology, Dong-Eui University) ;
  • Kim, Kwang-Hyeon (Department of Life Science and Biotechnology, Dong-Eui University)
  • Published : 2006.10.31

Abstract

To screen for an anti-yeast substance (AYS), many bacteria were isolated from soil and a strain AY2000 was selected. The strain AY2000 was identified as Rahnella aquatilis by morphology, biochemical properties, and 16S r-RNA nucleotide sequence analyses. The strain AY2000 showed anti-yeast activity against Candida albicans and Saccharomyces cerevisiae, whereas R. aquatilis ATCC33071 as a type strain did not show the activity against the yeasts under the same condition. The growth of yeast cell was significantly inhibited by AYS produced by the strain AY2000, as shown by optical density and MTT assay. The minimum inhibitory concentration (MIC) of the AYS against S. cerevisiae and C. albicans at $28^{\circ}C\;was\;20{\mu}g/ml\;and\;60{\mu}g/ml$, respectively. The MIC of AYS against hyphae of C. albicans at $37^{\circ}C\;was\;600{\mu}g/ml$. Scanning electron microscopic analysis revealed that yeast cells treated with AYS had an irregular form with a wrinkled and rough surface.

Keywords

References

  1. Berge, O., T. Heulin, W. Achouak, C. Richard, R. Bally, and J. Balandreau. 1991. Rahnella aquatilis, a nitrogen-fixing enteric bacterium associated with the rhizosphere of wheat and maize. Can. J. Microbiol. 37: 195-203 https://doi.org/10.1139/m91-030
  2. Caroff, N., C. Chamoux, F. Le Gallou, E. Espaze, F. Gavini, D. Gautreau, H. Richet, and A. Reynaud. 1998. Two epidemiologically related cases of Rahnella aquatilis bacteremia. Eur. J. Clin. Microbiol. Infect. Dis. 17: 349-352
  3. Cremer, A. 1984. Antibiotic sensitivity and assay tests, pp. 167-181. In C. H. Collins and P. M. Lyne. (eds.), Microbiological Methods, 5th Ed. Butterworths, London
  4. El-hendawy, H. H., M. E. Osman, and N. M. Sorour. 2003. Characterization of two antagonistic strains of Rahnella aquatilis isolated from soil in Egypt. Folia Microbiol. 48: 799-804 https://doi.org/10.1007/BF02931517
  5. Farmar III, J. J. 1984. Other genera of the family Enterobacteriaceae, pp. 506-513. In N. R. Krieg and J. G. Holt (eds.), Bergey's Manual of Systematic Bacteriology, 9th Ed. Williams and Wilkins, Baltimore/London
  6. Fridkin, S. K. and W. R. Jarvis. 1996. Epidemiology of nosocomial fungal infections. Clin. Microbiol. Rev. 9: 499-511
  7. Ghannoum, M. A. and L. B. Rice. 1999. Antifungal agents: Mode of action, mechanisms of resistance, and correlation of these mechanisms with bacterial resistance. Clin. Microbiol. Rev. 12: 501-517
  8. Hashidoko, Y., E. Itoh, K. Yokota, T. Yoshida, and S. Tahara. 2002. Characterization of five phyllosphere bacteria isolated from Rosa rugosa leaves, and their phenotypic and metabolic properties. Biosci. Biotechnol. Biochem. 66: 2474-2478 https://doi.org/10.1271/bbb.66.2474
  9. Hawser, S., M. Francolini, and K. Islam. 1996. The effects of antifungal agents on the morphogenetic transformation by Candida albicans in vitro. J. Antimicrob. Chemother. 38: 579-587 https://doi.org/10.1093/jac/38.4.579
  10. Hawser, S. and K. Islam. 1999. Comparison of the effects of fungicidal and fungistatic antifungal agents on the morphogenetic transformation of Candida albicans. J. Antimicrob. Chemother. 43: 411-413 https://doi.org/10.1093/jac/43.3.411
  11. Hwang, E. I., B. S. Yun, W. H. Yeo, S. H. Lee, J. S. Moon, Y. K. Kim, S. J. Lim, and S. U. Kim. 2005. Compound IKD-8344, a selective growth inhibitor against the mycelial form of Candida albicans, isolated from Streptomyces sp. A6702. J. Microbiol Biotechnol. 15: 909-912
  12. Kim, K. Y., D. Jordan, and H. B. Krishnan. 1997. Rahnella aquatilis, a bacterium isolated from soybean rhizophere can solubilize hydroxyapatite. FEMS Microbiol. Lett. 153: 273-277 https://doi.org/10.1016/S0378-1097(97)00246-2
  13. Kim, N. R., E. I. Hwang, B. S. Yun, S. H. Lee, J. S. Moon, C. H. Lim, S. J. Lim, and S. U. Kim. Isolation of Candida albicans chitin synthase 1 inhibitor from Streptomyces sp. A67-5 and its characterization. J. Microbiol Biotechnol. 15: 895-898
  14. Maidak, B. L., G. J. Olsen, N. Larsen, R. Overbeek, M. J. McCaughey, and C. R. Woese. 1996. The ribosomal database project (RDP). Nucleic Acids Res. 24: 82-85 https://doi.org/10.1093/nar/24.1.82
  15. Matsuyama, H., R. Sasaki, K. Kawasaki, and I. Yumoto. 1999. Production of a novel exopolysaccharide by Rahnella aquatilis. J. Biosci. Bioeng. 87: 180-183 https://doi.org/10.1016/S1389-1723(99)89009-4
  16. National Committee for Clinical Laboratory Standards. 1995. Reference Method for Broth Dilution Antifungal Susceptibility Testing of Yeasts: Tentative Standard M27-T. NCCLS, Vilanova, PA
  17. Nomanbhoy, F., C. Steele, J. Yano, and P. L. Fidel Jr. 2002. Vaginal and oral epithelial cell anti-cancer activity. Infect. Immun. 70: 7081-7088 https://doi.org/10.1128/IAI.70.12.7081-7088.2002
  18. Sanglard, D., K. Kuchler, F. Ischer, J. L. Pagani, M. Monod, and J. Bille. 1995. Mechanisms of resistance to azole antifungal agents in Candida albicans isolates from AIDS patients involve specific multidrug transporters. Antimicrob. Agents Chemother. 39: 1526-1529 https://doi.org/10.1128/AAC.39.7.1526
  19. Shin, D. S., S. H. Kim, H. C. Yang, and K. B. Oh. 2005. Cloning and expression of isocitrate lyase, a key enzyme of the glyoxylate cycle of Candida albicans, for development of antifungal drugs. J. Microbiol. Biotechnol. 15: 652-655
  20. Steele, C., J. Leigh, R. Swoboda, H. Ozenci, and P. L. Jr. Fider. 2001. Potential role for a carbohydrate moiety in anti-Candida activity of human oral epithelial cells. Infect. Immun. 69: 7091-7099 https://doi.org/10.1128/IAI.69.11.7091-7099.2001
  21. Yang, H.-C., Y. Mikami, K. Yazawa, H. Taguchi, K. Nishimura, M. Miyaji, M. L. M. Branchini, F. H. Aoki, and K. Yamamoto. 1998. Colorimetric MTT assessment of antifungal activity of D0870 against fluconazole-resistant Candida albicans. Mycosis 41: 77-480 https://doi.org/10.1111/j.1439-0507.1998.tb00382.x