Journal of Microbiology

The Microbiological Society of Korea (한국미생물학회)
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Nucleocapsid Amino Acids 211 to 254, in Particular, Tetrad Glutamines, are Essential for the Interaction Between the Nucleocapsid and Membrane Proteins of SARS-Associated Coronavirus

  • Fang, Xiaonan (State Key Laboratory of Virology, College of Life Science, Wuhan University) ;
  • Ye, Lin-Bai (State Key Laboratory of Virology, College of Life Science, Wuhan University) ;
  • Zhang, Yijuan (State Key Laboratory of Virology, College of Life Science, Wuhan University) ;
  • Li, Baozong (State Key Laboratory of Virology, College of Life Science, Wuhan University) ;
  • Li, Shanshan (State Key Laboratory of Virology, College of Life Science, Wuhan University) ;
  • Kong, Lingbao (State Key Laboratory of Virology, College of Life Science, Wuhan University) ;
  • Wang, Yuhua (State Key Laboratory of Virology, College of Life Science, Wuhan University) ;
  • Zheng, Hong (State Key Laboratory of Virology, College of Life Science, Wuhan University) ;
  • Wang, Wei (State Key Laboratory of Virology, College of Life Science, Wuhan University) ;
  • Wu, Zhenghui (State Key Laboratory of Virology, College of Life Science, Wuhan University)
  • Published : 2006.10.31
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Abstract

GST pull-down assays were used to characterize the SARS-CoV membrane (M) and nucleocapsid (N) interaction, and it was found that the amino acids 211-254 of N protein were essential for this interaction. When tetrad glutamines (Q) were replaced with glutamic acids (E) at positions of 240-243 of the N protein, the interaction was disrupted.

Keywords

References

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