Journal of the Korean Association of Oral and Maxillofacial Surgeons

  • 제32권2호
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  • Pages.107-116
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  • 2006
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  • 2234-7550(pISSN)
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  • 2234-5930(eISSN)
대한구강악안면외과학회 (The Korean Association of Oral and Maxillofacial Surgeons)
권호 표지

가토 상악동에 이식된 ${\beta}$-TCP의 골치유에 관한 실험적 연구

THE EXPERIMENTAL STUDY OF THE BONE REGENERATION ON RABBIT MAXILLARY SINUS GRAFTING WITH ${\beta}$-TCP

  • 박정하 (한양대학교 의과대학 치과학교실 구강악안면외과) ;
  • 황경균 (한양대학교 의과대학 치과학교실 구강악안면외과) ;
  • 박창주 (한양대학교 의과대학 치과학교실 구강악안면외과) ;
  • 최용수 (한양대학교 의과대학 치과학교실 구강악안면외과) ;
  • 마평수 (한양대학교 의과대학 치과학교실 구강악안면외과) ;
  • 백승삼 (한양대학교 의과대학 병리학교실) ;
  • 심광섭 (한양대학교 의과대학 치과학교실 구강악안면외과)
  • Park, Jung-Ha (Division of Oral & Maxillofacial Surgery, Department of Dentstry, College of Medicine Hanyang University) ;
  • Hwang, Kyung-Gyun (Division of Oral & Maxillofacial Surgery, Department of Dentstry, College of Medicine Hanyang University) ;
  • Park, Chang-Joo (Division of Oral & Maxillofacial Surgery, Department of Dentstry, College of Medicine Hanyang University) ;
  • Choi, Yong-Soo (Division of Oral & Maxillofacial Surgery, Department of Dentstry, College of Medicine Hanyang University) ;
  • Ma, Pyung-Soo (Division of Oral & Maxillofacial Surgery, Department of Dentstry, College of Medicine Hanyang University) ;
  • Paik, Seung-Sam (Department of Pathology, College of Medicine Hanyang University) ;
  • Shim, Kwang-Sup (Division of Oral & Maxillofacial Surgery, Department of Dentstry, College of Medicine Hanyang University)
  • 발행 : 2006.04.30
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초록

Purpose:Maxillay sinus grafting is an effective treatment procedure to improve bone height in the posterior maxillar area for implant installation. Beta-tricalciumphosphate(${\beta}$-TCP) was introduced to be grafting substitute material, providing a reasonable bio-degradation time, no need for harvesting procedure. The purpose of this study is to evaluate bone healing & regeneration phase using histomorphometric and immunohistochemical analysis. Material & Methods:Sixteen rabbits were divided into 4 groups. Bi-lateral maxillary sinus membranes were elevated at each rabbits, ${\beta}$-TCP was augmented in left sinus, autogenous bone was augmented in right sinus. The rabbits were sacrificed at 2, 4, 8 and 12 weeks. We investigated the bone regeneration & growth factor expression. Results: 1. The mean new bone volume formation was 28.99${\pm}$6.55%, 49.54${\pm}$5.47%, 69.09${\pm}$8.90% in autogenous grafted area, and 22.86${\pm}$5.56%, 24.00${\pm}$4.09%, 34.11${\pm}$3.37% in ${\beta}$-TCP area at 4, 8, 12 weeks. Therefore, new bone formation in autogenous bone was significantly higher than ${\beta}$-TCP (p<0.05). 2. The BMP 2/4 expression in autogenous bone grafted area was higher at 4, 8 weeks. 3. There was no difference in expression pattern of BMP-7/PDGF/VEGF during grafted bone regeneration. Conclusion:The authors we conclude that the autogenous bone graft was faster than ${\beta}$-TCP in bone regeneration, and the BMP 2/4 were more important in graft bone regeneration.

키워드

참고문헌

  1. Boyne PJ, James R: Grafting of the maxillary sinus floor with autogenous marrow and bone. J Oral Surg 1980;38:613-618
  2. Tatum OH: Maxillary and sinus implant reconstruction. Dent Clin North Am 1976;30:207-229
  3. Wood RM, Moore DL: Grafting of the maxillary sinus with intraorally harvested autogenous bone prior to implant placement. Int J Oral Maxillofac Implants 1988;3:209-214
  4. Laurencin CT: Bone Graft Substitutes. ASTM International. 2003. pp.180-187
  5. Merten HA, Wiltfang J, Grohmann U et al: Intraindividual Comparative Animal Study of $\alpha$-and $\beta$-Tricalcium Phosphate Degradation in Conjunction with Simultaneous Insertion of Dental Implants. J Craniofac Surg 2001;12:59-68 https://doi.org/10.1097/00001665-200101000-00010
  6. Szabo G, Suba Z, Hrabak K et al: Autogenous Bone Versus $\beta$- Tricalcium Phosphate Graft Alone for Bilateral Sinus Elevations (2- and 3-Dimensional Computed Tomographic, Histologic, and Histomorphometric Evaluations): Preliminary Results. Int J Oral Maxillofac Implant 2001;16:681-692
  7. Bahn S: Plaster: A Bone substitute. Oral Surg, Oral Med Oral Pathol 1966;21:672-681 https://doi.org/10.1016/0030-4220(66)90045-4
  8. Saito M, Shimizu H, Beppu M et al: The role of $\beta$-Tricalcium Phosphate in vasculized periosteum. J Orthop Sci 2000;5:275-282 https://doi.org/10.1007/s007760050163
  9. Watanbe K, Niimi A, Ueda M: Autogenous bone graft in the rabbit maxillary sinus. Oral Surg Oral Med Oral Radiol Endod 1999;88:26- 32 https://doi.org/10.1016/S1079-2104(99)70189-7
  10. Ogose A, Hotta T, Hatano H et al: Histological examination of $\beta$-TCP graft in human femur. J Biomed Mater Res 2002;63:601-604 https://doi.org/10.1002/jbm.10380
  11. Lu J, Descamps M, Dejou J et al: The Biodegradation Mechanism of Calcium Phosphate Biomaterials in Bone. J Biomed Mater 2002;63:408-412 https://doi.org/10.1002/jbm.10259
  12. de Leonardis D, Pecora GE: Augmentation of the maxillary sinus with calcium sulfate: histological results. J Periodontol 2000;30:153- 156
  13. Jensen OT: The Sinus Bone Graft. Quintessence. Publishing Co, Inc. 1999. pp.1-6, 17-31, 79-95
  14. Ducheyne P, Qiu Q: Bioactive ceramics : The effect of surface reactivity on bone formation and bone cell function. Biomaterials 1999;20:2287-2303 https://doi.org/10.1016/S0142-9612(99)00181-7
  15. Paolo T, Walter R, Alberto R et al: Histoiogic Effect of Pure-phase Beta-Tricalcium phosphate on Bone Regeneration in Human Artifical Jawbone Defects. Int J Periodontics Restorative Dent 2003;23:69-77
  16. Alex MG, Joachim P: Craniomaxillofacial Reconstructive and Corrective Bone Surgery. Springer International 2002. pp.174-209
  17. Watanabe K, Niimi A, Ueda M: Autogenous bone grafts in the rabbit maxillary sinus. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 1999;88:26-32 https://doi.org/10.1016/S1079-2104(99)70189-7
  18. Bolander MR: Regeneration of fracture repair by growth factors. Proc Soc Exp Bio Med 1992;2000:165-170
  19. Taylor JC, Cuff SE, Leger JP et al: In vitro osteoclast resorption of bone substitute biomaterials used for implant site augmentation : A pilot study. Int J Oral Maxillofac Implant 2002;17:321-330
  20. Flautre B, Maynou C, Lemaitre J et al: Bone colonization of $\beta$-TCP granules incorporated in brushite cements. J Biomed Mater Res 2002;63:413-417 https://doi.org/10.1002/jbm.10262
  21. Kloen P, Di paola M, Borens O et al: BMP signaling components are expressed in human fracture callus. Bone 2003;33:362-371 https://doi.org/10.1016/S8756-3282(03)00191-1
  22. Stenport VF, Johansson C, Heo SJ et al: Titanium implants and BMP- 7 in bone: an experimental model in the rabbit. J Mater Sci Mater Med 2003;14:247-254 https://doi.org/10.1023/A:1022884724059
  23. Urist MR, Raskin K, Goltz D et al: Endogenous bone morphogenic protein : immunohistochemical localization in repair of a punch hole in the rabbit's ear. Plast Reconstr Surg 1997;99:1382-1389 https://doi.org/10.1097/00006534-199704001-00028
  24. Trisi P, Rao W, Rebaudi A et al: Histologic effect of pure-phase betatricalcium phosphate on bone regeneration in human artificial jawbone defects. Int J Periodontics Restorative Dent 2003;23:69-77
  25. SH Lee, SI Song, KG Hwang et al: The experimental study of the bone regeneration on $\beta$-TCP in rabbit cranial bone. J Kor Oral Maxillofac Surg 2004;30:282-291
  26. Hai J, Li ST, Lin Q: Vascular Endotherial Growth Factor Expression and Angiogenesis Induced by Chronic Cerebral Hypoperfusion in Rat Brain. Neurosurgery 2003;53:963-978 https://doi.org/10.1227/01.NEU.0000083594.10117.7A
  27. Street J, Bao M, deGuzman L et al: Vascular endotherial growth factor stimulates bone repair by promoting angiogenesis and bone turnover. PNAS 2002;99:9656-9661