Journal of the Korean Association of Oral and Maxillofacial Surgeons

  • 제32권1호
  • /
  • Pages.27-35
  • /
  • 2006
  • /
  • 2234-7550(pISSN)
  • /
  • 2234-5930(eISSN)
대한구강악안면외과학회 (The Korean Association of Oral and Maxillofacial Surgeons)
권호 표지

Expression of transforming growth factor-1 in bone regeneration after the implantation of particulate dentin and plaster of Paris

  • Huh, Young-Chul (Department of Oral & Maxillofacial Surgery, College of Dentistry, Chonsun University) ;
  • Kim, Su-Gwan (Department of Oral & Maxillofacial Surgery, College of Dentistry, Chonsun University) ;
  • Kim, Jeong-Sun (Department of Oral & Maxillofacial Surgery, College of Dentistry, Chonsun University) ;
  • Yoon, Jung-Hoon (Department of Oral Pathology, College of Dentistry, Chonsun University) ;
  • Kim, Do-Kyung (Department of Oral Physiology, College of Dentistry, Chonsun University)
  • 발행 : 2006.02.28
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Purpose: This study was performed to investigate the expression of the transforming growth factor (TGF)-1, in a rat calvarium defect model using particulate dentin and/or plaster of Paris, and correlate the bone regeneration process with the histologic events. Materials and Methods: Thirty-two Sprague-Dawley rats were divided into 4 groups of 8 animals each. A 1.0 cm-sized calvarial defects were made and the defect was filled with different graft materials as follows : Group A, the defects were filled with a mixture of particulate dentin and plaster of Paris with a 2:1 ratio; Group B, the defects were filled with plaster of Paris only; Group C, defects were filled with particulate dentin only; Group D, untreated control group. The animals were sacrificed by 1, 2, 4, 8 weeks after implantation. Excised wound tissues were processed for histology, immunohistochemistry and RT-PCR for the analysis of TGF-1 expression. Results: Gene expression of TGF-1 was detected for all experimental groups. The highest gene expression was observed in the specimen taken at the first week after implantation in Group A. According to the histologic and immunohistochemical studies, TGF-1 positive osteoblast-like cells were found in the early stage of healing after the implantation of particulate dentin and plaster of Paris. Conclusion: These findings suggest that TGF-1 may be related to new bone formation at the early healing process after the implantation of particulate dentin and plaster of Paris.

키워드

참고문헌

  1. Kim SG, Yeo HH, Kim YK: Grafting of large defects of the jaws with a particulate dentin-plaster of Paris combination. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 1999;88:22 https://doi.org/10.1016/S1079-2104(99)70188-5
  2. Kim SG, Kim HK, Lim SC: Combined implantation of particulate dentin, plaster of Paris, and a bone xenograft (Bio- Oss) for bone regeneration in rats. J Cranio-Maxillofac Surg 2001;29:282 https://doi.org/10.1054/jcms.2001.0236
  3. Kim SG, Chung JH, Kim YK, et al: The use of particulate dentin-plaster of Paris combination with/without plateletrich plasma in the treatment of bone defects around implants. Int J Oral Maxillofac Implants 2002;60:82 https://doi.org/10.1053/joms.2002.29080
  4. Kim SG, Kim WG, Park JC, et al: A comparative study of osseointegration of AVANA implants in a demineralized freeze-dried bone alone or with platelet-rich plasma. J Oral Maxillofac Surg 2002;60:1018 https://doi.org/10.1053/joms.2002.34413
  5. Kim SY, Kim SG, Lim SC, et al: Effects on bone formation in ovariectomized rats after implantation of particulate dentin and plaster of Paris mixture. J Oral Maxillofac Surg 2004;62:8523
  6. Moghaddam H, Sandor GKB, Holmes HHI, et al: Histomorphometric evaluation of bone regeneration using allogeneic and alloplastic bone substitutes. J Oral Maxillofac Surg 2004;62:202 https://doi.org/10.1016/j.joms.2003.10.002
  7. Kanyama M, Kuboki T, Akiyama K, et al: Connective tissue growth factor expressed in rat alveolar bone regeneration sites after tooth extraction. Arch Oral Biol 2003;48:723 https://doi.org/10.1016/S0003-9969(03)00153-5
  8. Yates KE, Troulis MJ, Kaban LB, et al: IGF-1, TGF-, and BMP-4 are expressed during distraction osteogenesis of the pig mandible. Int J Oral Maxillofac Surg 2002;31:173 https://doi.org/10.1054/ijom.2001.0204
  9. Yeung HY, Lee KM, Fung KP, et al: Sustained expression of transforming growth factor-1 by distraction during distraction osteogenesis. Life Sci 2002;71:67 https://doi.org/10.1016/S0024-3205(02)01575-8
  10. Lalani Z, Wong M, Meng EMB, et al: Spatial and temporal localization of transforming growth factor-1, bone morphogenic protein-2, and platelet-derived growth factor-A in healing tooth extraction sockets in a rabbit model. J Oral Maxillofac Surg 2003;61:1061 https://doi.org/10.1016/S0278-2391(03)00319-7
  11. Urist MR: Bone: formation by autoinduction. Science 1965;150:893 https://doi.org/10.1126/science.150.3698.893
  12. Sakou T: Bone morphogenetic proteins: from basic studies to clinical approaches. Bone 1998;22: 591 https://doi.org/10.1016/S8756-3282(98)00053-2
  13. Joyce ME, Roberts AB, Sporn MB, et al: Transforming growth factor-beta and the initiation of chondrogenesis and osteogenesis in the rat femur. J Cell Biol 1990;110:2195 https://doi.org/10.1083/jcb.110.6.2195
  14. Wang EA, Rosen V, D'Alessandro JS, et al. Recombinant human bone morphogenetic protein induces bone formation. Proc Natl Acad Sci USA 1990;87:2220 https://doi.org/10.1073/pnas.87.6.2220
  15. Ryseck RP, Macdonald-Bravo H, Mattei MG, et al: Structure, mapping, and expression of fisp-12, a growth factor-inducible gene encoding a secreted cysteine-rich protein. Cell Growth Differ 1991;2:225
  16. Bork P: The modular architecture of a new family of growth regulators related to connective tissue growth factor. FEBS Lett 1993;327:125 https://doi.org/10.1016/0014-5793(93)80155-N
  17. Brigstock D: The connective tissue growth factor/cysteinerich/ nephroblastoma overexpressed (CCN) family. Endocr Rev 1999;20:189 https://doi.org/10.1210/er.20.2.189
  18. Lau LF, Lam SC: The CCN family of angiogenic regulators the integrin connection. Exp Cell Res 1999;248:44-57 https://doi.org/10.1006/excr.1999.4456
  19. Sporn MB, Roberts AB. The multifunctional nature of growth factors. In: Sporn MB, Roberts AB, editors. Handbook of experimental pharmacology: Peptide growth factors and their receptors. New York: Springer-Verlag 1990:3-15
  20. Robey PG, Young MF, Flanders KC, et al: Osteoblasts synthesize and respond to transforming growth factor-type beta (TGF-beta) in vitro. J Cell Biol 1987;105:457 https://doi.org/10.1083/jcb.105.1.457
  21. Lind M, Schumacker B, Soballe K, et al: Transforming growth factor-beta enhances fracture healing in rabbit tibiae. Acta Orthopaed Scand 1993;64:553 https://doi.org/10.3109/17453679308993691
  22. Terrell TG, Working PK, Chow CP, et al: Pathology of recombinant human transforming growth factor-beta 1 in rats and rabbits. Int Rev Exp Pathol 1993;34:43