Analysis of Lignans in Acanthopanax sessiliflorus Fruits and Their Fermented Wine by HPLC

  • Kim, Hye-Min (Department of Applied Plant Science, College of Industrial Science, Chung-Ang University) ;
  • Kim, Ju-Sun (Natural Products Research Institute and College of Pharmacy, Seoul National University) ;
  • Cho, Seon-Haeng (Gongju National University of Education) ;
  • Kang, Sam-Sik (Natural Products Research Institute and College of Pharmacy, Seoul National University) ;
  • Cheoi, Dae-Sung (Jeongseon Agricultural Technology & Extension Center) ;
  • Lee, Sang-Hyun (Department of Applied Plant Science, College of Industrial Science, Chung-Ang University)
  • Published : 2006.10.01

Abstract

High performance liquid chromatography (HPLC) was used for the determination of lignans, eleutherosides B and E, in Acanthopanax sessiliflorus fruits and their fermented wine. The lignans were quantified by a reversed-phase system using a gradient of $H_2O$ and acetonitrile as a mobile phase within 20 min. The analysis was successfully carried out within 20 min. The contents of eleutherosides Band E as main active principles of Acanthopanax species were measured in A. sessiliflorus fruits (1.15 and $8.49\;{\mu}g/mg$, respectively), their fermented wine (0.45 and $1.33\;{\mu}g/mg$, respectively) and wine residues (no detection).

Keywords

References

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