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키토산-아스코베이트의 용해성, 항산화성 및 항균성

Solubility, Antioxidative and Antimicrobial Activity of Chitosan-Ascorbate

  • 이승배 (대구가톨릭대학교 식품외식산업학부 식품공학) ;
  • 이예경 (대구가톨릭대학교 식품외식산업학부 식품공학) ;
  • 김순동 (대구가톨릭대학교 식품외식산업학부 식품공학)
  • Lee, Seung-Bae (Dept. of Food Science and Technology, Food Industrial Technology, Catholic University of Daegu) ;
  • Lee, Ye-Kyung (Dept. of Food Science and Technology, Food Industrial Technology, Catholic University of Daegu) ;
  • Kim, Soon-Dong (Dept. of Food Science and Technology, Food Industrial Technology, Catholic University of Daegu)
  • 발행 : 2006.08.30

초록

동결건조한 chitosan-ascorbate(CAs)와 chitosan-acetate(CAc)의 용해성, 항균성, 항산화성을 비교하였다. 용해성을 조사한 결과 CAs는 증류수, 식초, 녹차, 소주, 맥주, 적포도주에 0.5%이상의 농도로 용해되었으나 간장, 두유, 우유, 오렌지주스, 커피, 참기름, 대두유에는 녹지 않았다. CAc도 CAs의 경우와 비슷하나 맥주에서는 0.1%이하의 농도에서 용해되었으며 적포도주에서는 커드를 형성하였다. CAc의 전자공여능, 항산화능 및 SOD활성은 CAc에서는 각각 0, 40.0 및 10.0%였으나 CAs의 경우는 48.2, 90.6 및 67.5%로 CAc보다 높은 활성을 나타내었다. B. circulans, B. brevis, B. licheniformis, B. arabitane, B. sterothermophillus에 대한 CAs와 CAc의 최소저해농도(MIC)는 다같이 $200\;{\mu}g/disc$이었으며, E. coli O157, Listeria monocytogenous, B. cereus, B. subtilis에 대한 MIC는 다같이 $400\;{\mu}g/disc$으로 뚜렷한 차이를 보이지 않았다. CAs와 CAc의 Hunter's L*값은 $81.95{\sim}82.97$로 뚜렷한 차이가 없었으나 Hunter's a* 및 b*값은 CAs가 높았다. 관능검사 결과, CAs는 CAc에 비하여 신맛과 쓴맛은 낮았으나 떫은맛은 뚜렷한 차이가 없었다. 결론적으로 CAs는 CAc에 비하여 항균성, 항산화성 및 기호도 측면에서 우수하여 식품에의 활용이 기대된다.

This study was conducted to investigate the solubility, antioxidative and antimicrobial activity of the freeze dried chitosan-ascorbate (CAs) and chitosan-acetate (CAc). In the results of solubility, CAs was soluble over 0.5% in distilled water, vinegar, green tea, soju (distilled liquor), beer and red wine, while it was not soluble in soy sauce, soy milk, milk, orange juice, coffee, sesame oil, soy milk and soybean oil. The solubility of CAc in the liquid foods was similar to those of CAs, but it was soluble less than 0.1% in beer, and formed curd in red wine. Electron donating activity, antioxidative activity and SOD activity of CAs were 48.2, 90.6 and 67.5%, respectively, while the activities of the CAc were 0, 40.0 and 10.0%, respectively. The minimal inhibitory concentrations of CAs and CAc were $200\;{\mu}g/disc$ against Bacillus circulans, Bacillus brevis, Bacillus licheniformis, Bacillus arabitane and Bacillus sterothermophillus, $400\;{\mu}g/disc$ against Escherichia coli O157, Listeria monocytogenous, Bacillus cereus and Bacillus subtilis. There was no significant difference in Hunter's L* value between CAs and CAc $(81.95{\sim}82.97)$, but Hunter's a* and b* values of the CAs was higher than those of CAc. While sour and bitter tastes of CAs were lower than those of CAc, there was no significant difference in astringent taste. From these results, it suggested that CAs has more extensive utility in liquid foods with antimicrobial and antioxidant activity as well as sensory quality compared to CAc.

키워드

참고문헌

  1. Tsujikawa T, Kanauchi O, Andoh A, Saotome T, Sasaki M, Fujiyama Y, Bamba T. 2003. Supplement of a chitosan and ascorbic acid mixture for Crohn's disease. A pilot study. Nutr 19: 137-139 https://doi.org/10.1016/S0899-9007(02)00958-9
  2. Muzzarelli RAA. 1977. Chitin. Pergamon Press, Oxford, UK. p 5-9
  3. Nishimura K, Nishihara S, Nishi N, Tokura S, Azuma I. 1984. Immunological activity of chitin and its derivatives. Vaccine 2: 93-99 https://doi.org/10.1016/S0264-410X(98)90039-1
  4. Kojima K, Yoshikuni M, Suzuki T. 1979. Tributyl-borane-initiated grafting of methyl methacryalte onto chitin. J Appl Polym Sci 24: 1587-1593 https://doi.org/10.1002/app.1979.070240701
  5. Jung BO, Chung TS. 1998. Studies on the development of polymeric flocculants of chitosan system. J Korean Ind Eng Chem 9: 451-456
  6. Knorr D. 1984. Use of chitinous polymers in food. A challenge for food research and development. Food Technol 38: 85-97
  7. Sanford PA. 1988. Chitosan, commercial uses and potential applications. Proc. the 4th International Conference on chito/chitosan held in Trondhim, Norway. p 51-69
  8. Hirano S, Kondo Y, Fujii K. 1985. Preparation of acetylated derivatives of modified chito-oligosaccharides by the depolymerization of partially N-acetyled chitosan with nitrous acid. Carbohydr Res 163: 338-341
  9. Akiyama K, Kawazu K, Kobayash A. 1995. A novel method for chemo-enzymatic synthesis of elicitor-action chitosan oligomers and partially N-deacetylated chitin oligomers using N-acylated chitotrioses as substrates in a lysozyme-catalyzed transglycosylation reaction system. Carbohydr Res 279: 151-160 https://doi.org/10.1016/0008-6215(95)00288-X
  10. Muzzarelli RAA, Tanfani F, Emanuelli M. 1984. Chelating derivatives of chitosan obtained by reaction with ascorbic acid. Carbohydr Polym 4: 137-151 https://doi.org/10.1016/0144-8617(84)90020-1
  11. Kanauchi O, Deuchi K, Imasato Y, Shizukuishi M, Kobayashi E. 1994. Mechanism for the inhibition of fat digestion by chitosan and for the synergistic effect ascorbate. Biosci Biotech Biochem 59: 786-790
  12. Zoldners J, Kiseleva T, Kaiminsh I. 2005. Influence of ascorbic acid on the stability of chithosan solutions. Carbohydr Polymer 60: 215-218 https://doi.org/10.1016/j.carbpol.2005.01.013
  13. No HK, Lee KS, Kim ID, Park MJ, Kim SD, Meyer SP. 2005. Chitosan treatment affects yield, ascorbic acid content, and hardness of soybean sprouts. J Food Sci 68: 680- 685 https://doi.org/10.1111/j.1365-2621.2003.tb05731.x
  14. Lee MY, Lee YK, Kim SD. 2004. Quality characteristics of calcium acetate prepared with vinegars and ash of black snail. J Korean Soc Food Sci Nutr 33: 593-597 https://doi.org/10.3746/jkfn.2004.33.3.593
  15. Seo SB, You HJ, Seo CS. 2003. Antibacterial and anti-inflammatory compositions with Lnula helenium L. extract and water soluble chitosan. US Patent 6521268
  16. Blois MS. 1958. Antioxidant determination by the use of a stable free radical. Nature 181: 1199-1200 https://doi.org/10.1038/1811199a0
  17. Marklund S, Marklund G. 1974. Involvement of superoxide anion radical in the oxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem 47: 468- 474
  18. Bauer AW, Kirby WMM, Sherris JC, Turck M. 1966. Antibiotic susceptibility testing by a standardized single disc method. Am J Clin Pathol 6: 493-496
  19. Lee MY, Park SY, Jung KO, Park KY, Kim SD. 2005. Quality and functional characteristics of chungkukjang prepared with various Bacillus sp. isolated form traditional chungkukjang. J Food Sci 70: 191-196 https://doi.org/10.1111/j.1365-2621.2005.tb07187.x
  20. Meilgaard M, Civille GV, Carr BT. 1987. Sensory Evaluation Techniques. CRC Press, Inc., Boca Raton, Florida, USA. p 39-112
  21. Nishihara T, Kosugi M, Matsue Y, Nishikawa J, Takasaki A, Takubo Y. 1992. Anitmicrobial activity of positive colloids against food poisoning bacteria. J Antibacterial Antifungal Agents 20: 241-245
  22. Jung BO, Chung SJ, Lee YM, Kim JJ. 2001. Antimicrobial activity and antioxidative activity of water soluble chitosan. J Chitin Chitosan 6: 12-17
  23. Hahn HG, Nam KD. 2004. Fungicidal activities of chitosan against plant pathogens. J Chitin Chitosan 9: 73-78
  24. Kendra DF, Hadwiger LA. 1984. Characterization of the smallest chitosan oligomer that is maximally antifungal to Fusarium solani and elicits pisatin formation in Pisum sativum. Exp Mycol 8: 276-281 https://doi.org/10.1016/0147-5975(84)90013-6
  25. Darmadji P, Lzumimoto M. 1994. Effect of chitosan in meat preservation. Meat Sci 38: 243-254 https://doi.org/10.1016/0309-1740(94)90114-7
  26. Wang SW. 1992. Inhibition and inactivation of five species of foodborn pathogens by chitosan. J Food Prot 55: 916- 919 https://doi.org/10.4315/0362-028X-55.11.916
  27. Kim CH, Choi JW, Chun HJ, Choi KS. 1997. Synthesis of chitosan derivatives with quaternary ammonium salt and their antibacterial activity. Polym Bull 38: 387-393 https://doi.org/10.1007/s002890050064
  28. Kim DH, Lee C, Kim KO, Lee YC. 1999. Physicochemical and sensory properties of water soluble chitosan. Korean J Food Sci Technol 31: 83-90

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  1. Effects of Complex Carbohydrase Treatment on Physiological Activities of Pear Peel and Core vol.43, pp.3, 2014, https://doi.org/10.3746/jkfn.2014.43.3.404