Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Revision of the Analysis of Makgeolli for Ochratoxin A

막걸리 중 ochratoxin A 분석의 개선

  • Park, Je-Won (Food Research Division, Food Directorate, Health Products and Food Branch, Health Canada)
  • 박제원 (헬스 캐나다 식품 연구부)
  • Published : 2006.02.01
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Abstract

Because ochratoxin A recovery level of Makgeolli is lower (<50%) than those of other commodities such as rice, barley, and beer, Makgeolli was evaluated to improve the recovery and enable routine analysis. Use of 3% sodium bicarbonate instead of phosphate-buffered saline as homogenizing solution provided good recovery of ochratoxin A (>80%) spiked in Makgeolli at level of 1 ppb. To determine if this analytical method is reliable for ochratoxin A detection in Makgeolli, survey was conducted for ochratoxin A in 30 sterile Makgeolli samples retailed in Korea. Only two wheat-based Makgeolli samples contained detectable level of ochratoxin A (0.8 and 2.1 ppb) as confirmed by HPLC- electrospray ionization- mass spectrometry. Extraction of sample with 3% sodium bicarbonate for 3 min, followed by cleanup of extracts with immunoaffinity columns, and HPLC with fluorescence detection provided dependable detection of ochratoxin A in Makgeolli samples.

이전의 연구 결과에 나타난 바 있는 막걸리 중 ochratoxin A의 낮은 회수율(<50%)은 시료 내 본 곰팡이독소의 추출 혹은 전처리 과정의 개선이 필요함을 지적하였다. 본 연구에서 막걸리 시료의 균질화에 이용되던 추출 용액을 PBS 용액 대신 3% sodium bicarbonate 용액으로 채택할 경우, 80% 이상의 첨가된 ochratoxin A가 회수되어 그 검출이 용이해졌다. 한편 개선된 해당 분석법의 막걸리 시료에 대한 적용 가능성을 알아보기 위하여 국내 시판 중인 살균막걸리 30점을 대상으로 분석한 결과 밀 막걸리 2점에서 ochratoxin A가 검출되었고(0.8, 2.1 ppb), 그 결과를 다시 HPLC-ESI-MS로 확정하였다. 본 연구를 통해 수행된 개선된 시료 전처리 방법(3% sodium bicarbonate)과 병행된 IAC 및 HPLC-FD 분석법은 막걸리를 대상으로 한 ochratoxin A의 오염 조사에 유효한 분석법임이 확인되었다.

Keywords

References

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