Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
권호 표지

Analgesic Effect and Inhibition of Prostaglandin $E_2$ Activity and Pro-inflammatory Cytokines Production by Ethyl Alcohol Extract from New Herbal Formula

신규 생약 복합추출물에 의한 Prostaglandin $E_2$ 활성억제, 염증성 Cytokine 생성 저해 및 진통 작용에 관한 연구

  • 권순용 (가톨릭대학교 성모병원 정형외과) ;
  • 이화성 (가톨릭대학교 성모병원 정형외과) ;
  • 이순혁 (고려대학교 안암병원 정형외과) ;
  • 임군일 (동국대학교 일산병원 정형외과) ;
  • 김시나 ((주)KMSI 부설 한국의과학연구소) ;
  • 김희석 ((주)KMSI 부설 한국의과학연구소) ;
  • 황성완 ((주)KMSI 부설 한국의과학연구소) ;
  • 황성연 ((주)KMSI 부설 한국의과학연구소)
  • Published : 2006.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

New herbal formula (NHF) is the ethanol extract mixture of Puerariae radix, Artemisia capillaries and Perilla frutescens. We have Investigated the effects on anti-inflammation by NHF and attempted acetic acid induced writhing to verify the analgesic effect. Macrophages and chondrocytes were obtained from mouse and rabbit. Inflammation was induced bγ interleukin-1, tumor necrosis $factor-{\alpha}$, $interferon-{\gamma}$, and lipopolysaccharide. NHF showed strong inhibitory efficacy against cytokine-induced proteoglycan degradation, $PGE_2$ production, NO production, and MMP-9 expression in rabbit articular chondrocyte. In the writhing test, NHF exhibited a dose-dependent inhibition of writhing. Futhermore, NHF increased the activity of SOD. NHF have anti-inflammatory and analgesic activities, and could be a good herbal medicine candidate for curing of osteoarthritis.

Keywords

References

  1. Flower, R. J. (1999) New directions in cyclooxygenase research and their implications for NSAID-gastripathy. Ita.l J. Gastroenterol. 28(4): 23-27
  2. Huh, L. H., Kim, H. C. and Lee, S. J (1987) Studies on antiinflammatory activity and its mechanism of daidzein. J. Pharmaceut. Soc. Kor. 31(1): 164-163
  3. Lee, C. H., Han, S. H. and Min, S. G. (1995) The effects of Puerariae radix actechins administration on liver function in carbon tetrachloride-treated rats. J. Korean. Soc. Food. Sci. Nurt. 24(5): 713-719
  4. Lee, J. S., Kim, E. S. and Kim, S. W. (1999) Effects of extract of Perariae radix on lipid peroxidation in ethanol-administered rats. J. Korean. Soc. Food. Sci. Nurt. 28(4): 901-906
  5. Lin, R. C., Guthrie, S., Xie, C. Y. et al. (1996) Isoflavone compounds extracted from Pueraria lobata suppress alcohol preference in a pharmacogenetic rat model of alcoholism. Alcohol. Clin. Exp. Res. 20(4): 659-663 https://doi.org/10.1111/j.1530-0277.1996.tb01668.x
  6. Oh, M. J., Lee, K. S., Son, H. Y. and Kim, S. Y (1990) Antioxidative Components of Pueraria Root. J. Kor. Soc. Food. Sci. Tech. 22(7): 793-795
  7. Zeng, G.Y., Cheng, Y. S., Fan, L. L., Zhou, Y. P. and Zhang, L. Y. (1982) Pharmacologic studies on Radix puerariae: effects of puerariae flavones on coronary circulation, cardiac hemodynamics and myocardial metabolism in dogs. Chinese, Med. J. 95(2): 145-150
  8. Kobayashi, T., Nakata, T. and Kuzumaki, T. (2002) Effect of flavonoids on cell cycle progression in prostate cancer cells. Cancer Letters 176(1): 17-23 https://doi.org/10.1016/S0304-3835(01)00738-8
  9. Kim, H. K., Cheon, B. S., Kim, Y H., Kim, S. Y. and Kim, H. P. (1999) Effects of naturally occuning flavonoids on nitric oxide production in the macrophage cell line RAW 264.7 and their structure-activity relationships. Biochemical Pharmacology 58(5): 759-765 https://doi.org/10.1016/S0006-2952(99)00160-4
  10. Kobayashi, T., Nakata, T. and Kuzumaki, T. (2002) Effect of flavonoids on cell cycle progression in prostate cancer cells. Cancer Letters 176(1): 17-23 https://doi.org/10.1016/S0304-3835(01)00738-8
  11. Liang, Y., Huang, Y., Tsai, S., Lin-Shiau, S., Chen, C. and Lin, J. (1999) Suppression of inducible cyclooxygenase and inducible nitric oxide synthase by apigenin andrelated flavonoids in mouse macrophages. Carcinogenesis 20(10): 1645-1652 https://doi.org/10.1093/carcin/20.8.1645
  12. Sandy, J. D., Brwon, H. L. G. and Lowther, D. A. (1978) Degradation of proteoglycan in acticular cartilage. Biochem. Biophys. Acta 543: 539-544
  13. Badger, A. M., Cook, M. N., Swift, B. A., Newmantraa, T. M., Gowen, M. and Lark, M. (1999) Inhibition of Interleukin- 1 induced proteoglycan degradation and nitric oxide production in bovine articular cartilage/chondrocyte cultures by the natural product, Hymenialdisine. J. Pharmacol. Exp. Ther. 290: 587-593
  14. Elliott, S. and Cawston, T. (2002) The clinical potential of matrix metalloproteinase inhibitors in rheumatic disorders. Drug Aging. 18: 87-99 https://doi.org/10.2165/00002512-200118020-00002
  15. Farrell, A. J., Blake, D. R., Palmer, R. M. and Moncada, J. (1992) Increased concentrations of nitric synovial fluid and serum samples suggest increased nitric oxide synthesis in rheumatic diseases. Ann. Rheum. Dis. 51: 1219-1222 https://doi.org/10.1136/ard.51.11.1219
  16. Lee, S. H., Seo, G S. and Sohn, D. H. (2004) Inhibition of lipopolysaccharided-induced expression of inducible nitric oxide synthase by butein in RAW 264.7 cells. Biochem. Biophys. Res. Commun. 323: 125-132 https://doi.org/10.1016/j.bbrc.2004.08.063
  17. Nam, K. W, Je, K. G, Lee, J. G., Han, H. J., Kang, S. K. and Mar, W. C. (2003) Inhibition of COX-2 activity and proinflammatory cytokines (TNF$-{\alpha}$ and IL-$1{\beta}$) production by water-soluble sub-fractionated parts from Bee (Apis mellifera) Venom. Arch. Pharm. Res. 26: 383-388 https://doi.org/10.1007/BF02976695
  18. Park, B. J., Cho, M. G, Kim, K. G, et al. (1999) Effect of natural product on the inhibition of Lipopolysacchride-induced nitric oxide synthase activity in RAQ 264.7 cell culture system. Natural Product Sciences 5(3): 113-120
  19. Westacott, C. I. and Sharif, M. (1996) Cytokines in osteoarthritis: Mediators or markers of joint destruction. Semin. Arthritis. Rheum. 25: 254-272 https://doi.org/10.1016/S0049-0172(96)80036-9
  20. Moon, T. C., Chung, K. C., Son, K. H., Kim, H. P., Kang, S. S. and Chang, H. W. (1998) Screening of Cyclooxygenase-2 (COX-2) Inhibitors from Natural Products. Yakhak Hoeji 42(2): 214-219