Natural Product Sciences

The Korean Society of Pharmacognosy (한국생약학회)
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Suppression of Cyclooxygenase-2 Expression in Colonic Epithelial Cells by Ilekudinol B Isolated from Weigela subsessilis

  • Park, Hye-Jung (Department of Oriental Pharmacy, College of Pharmacy, Wonkwang University) ;
  • Choi, Yeon-A (Department of Oriental Pharmacy, College of Pharmacy, Wonkwang University) ;
  • Tae, Jin (Department of Oriental Pharmacy, College of Pharmacy, Wonkwang University) ;
  • Kang, Chon-Sik (Department of Oriental Pharmacy, College of Pharmacy, Wonkwang University) ;
  • Kim, Dae-Ki (Department of Immunolgy, Chonbuk National University Medical School) ;
  • Thuong, Phuong Thien (College of Pharmacy, Chungnam National University) ;
  • Kim, Young-Ho (College of Pharmacy, Chungnam National University) ;
  • Bae, Ki-Hwan (College of Pharmacy, Chungnam National University) ;
  • Lee, Young-Mi (Department Of Oriental Pharmacy, Colloge of Pharmacy, Wonkwang University)
  • Published : 2006.03.01
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Abstract

Ilekudinol B is one of the flavonoids isolated from Weigela subsessilis (Caprifoliaceae). In the present study, the suppression effect of ilekudinol B on tumor necrosis factor $(TNF)-{\alpha}-induced$ cyclooxygenase-2 (COX-2) expression was investigated in human colon epithelial cell line HT-29. Interleukin-8 (IL-8) production and prostaglandin $E_2\;(PGE_2)$ secretion was measured by enzyme-linked immunosorbent assay (ELISA). COX-2 and nuclear factor $(NF)-{\kappa}B$ expression were determined by Western blot analysis. Ilekudinol B significantly inhibited $TNF-{\alpha}-induced$ secretion of IL-8 and prostaglandin $E_2\;(PGE_2)$ from the human colon epithelial cell line HT-29 in a concentration-dependent manner. In addition, ilekudinol B remarkably diminished $TNF-{\alpha}-induced$ COX-2 expression and $NF-{\kappa}B$ p65 subunit translocation to the nucleus. In conclusion, our results indicate that ilekudinol B may have anti-inflammatory activity on $TNF-{\alpha}-dependent$ colonic inflammation.

Keywords

References

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