Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Screening of Endogenous Maize (Zea mays) Substances Enhancing Auxin-induced Inward Curvature in Coleoptilar Slits

안쪽으로 굽어지는 자엽초 박편의 옥신 반응을 촉진하는 옥수수(Zea mays) 내생물질의 탐색

  • Park, Woong-June (Graduate Program for RNA Biology, Department of Molecular Biology, Institute of Nanosensor and Biotechnology, Dankook University)
  • 박웅준 (단국대학교 분자생물학과, 나노센서 바이오텍 연구소, BK2l RNA 전문인력양성사업팀)
  • Published : 2006.08.30
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Abstract

When thin slits (e.g., $1mm{\times}10mm$) of maize (Zea mays) coleoptiles were floated on a buffer, they spontaneously curved outward because of unbalanced tissue tension between inner and outer faces. Exogenously applied auxin induced inward curvature of the thin strip of the maize coleoptile in a dose-dependent manner. This bioassay system was used to screen endogenous substances that work together with auxin. In methanol extract of maize coleoptiles including the leaves inside, Active fractions that promote the auxin-induced inward curvature of maize coleoptile slices were found. The curvature-enhancing activity of the extract was not related to energy supply. The active substances were adsorbed to $C_{18}$ cartridges even at pH 10 and eluted in two fractions by 50% and 80% methanol. These substances were named as Curvature-Enhancing Factor-1 (CEF-1) and Curvature-Enhancing Factor-2 (CEF-2), respectively. The CEF-2 was resolved on a reversed phase $C_{18}$ column by HPLC.

옥수수(Zea mays) 자엽초 박편을 분리하여 완충용액에 띄워 배양하면 조직 내부와 외부 사이의 장력 차이에 의하여 밖으로 굽어진다. 그러나 외부에서 옥신을 처리하면 안으로 굽어지는 반응이 나타나는데, 그 반응 정도는 옥신 농도에 따라 변화하였다. 본 연구에서는 옥신에 의하여 자엽초 박편이 안으로 굽어지는 현상을 옥신과 함께 작용하는 물질들을 선별하기 위한 실험에 이용하였다. 옥수수 자엽초의 메탄올 추출물로부터, 옥신에 의해 안으로 굽어지는 자엽초 박편의 반응을 촉진하는 활성을 발견하였다. 이러한 촉진 활성은 에너지원과 관련된 현상은 아니었다. 활성물질은 pH 10과 같은 높은 pH에서도 $C_{18}$ 컬럼에 흡착되어 메탄올로 용리되었으며, 50% 메탄올과 80% 메탄올에 의하여 용리되는 두 가지 물질을 발견하고 각각 Curvature-Enhancing Factor-1 (CEF-1)과 Curvature-Enhancing Factor-2 (CEF-2)라 명명하였다. 그중 CEF-2의 HPLC를 이용한 분리 조건이 확립되었다.

Keywords

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