생명과학회지 (Journal of Life Science)

  • 제16권5호
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  • Pages.746-749
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  • 2006
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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구절초에서 분리한 Flavonoids의 인체암 세포주에 대한 세포독성 효과

Cytotoxic Flavonoids from the Whole Plants of Chrysanthemum zawadskii Herbich var. latilobum Kitamura

  • 권현숙 (경상대학교 응용생명과학과 & 농업생명과학연구원) ;
  • 하태정 (영남농업연구소) ;
  • 황선우 (경상대학교 응용생명과학과 & 농업생명과학연구원) ;
  • 진영민 (경상대학교 응용생명과학과 & 농업생명과학연구원) ;
  • 남상해 (진주 산업대학교 식품과학과) ;
  • 박기훈 (경상대학교 응용생명과학과 & 농업생명과학연구원) ;
  • 양민석 (경상대학교 응용생명과학과 & 농업생명과학연구원)
  • Kwon, Hyun-Sook (Division of Applied Life Science(BK21 program), Institute of Agriculture & Life Sciences, Gyeongsang National University) ;
  • Ha, Tae-Joung (Yeongnam Agricultural Research Institute, National Institute of Crop Science, RDA) ;
  • Hwang, Seon-Woo (Division of Applied Life Science(BK21 program), Institute of Agriculture & Life Sciences, Gyeongsang National University) ;
  • Jin, Young-Min (Division of Applied Life Science(BK21 program), Institute of Agriculture & Life Sciences, Gyeongsang National University) ;
  • Nam, Sang-Hae (Department of Food Science, Jinju National University) ;
  • Park, Ki-Hun (Division of Applied Life Science(BK21 program), Institute of Agriculture & Life Sciences, Gyeongsang National University) ;
  • Yang, Min-Suk (Division of Applied Life Science(BK21 program), Institute of Agriculture & Life Sciences, Gyeongsang National University)
  • 발행 : 2006.08.30
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초록

구절초 전초의 chloroform 분획물로부터 2종의 flavonoid화합물을 분리하여 NMR을 통해서 구조를 확인한 결과, luteolin (1)과 acacetin (2)으로 구조 동정되었다. 이들의 화합물 중에서 luteolin (1)은 구절초에서 처음으로 분리하였다. 분리된 화합물은 sulforhodamine B (SRB) assay법에 따라 인체암세포주인, HCT116 (결장암) , UO-31 (신장암), PC-3 (전립선암) 와 A549 (폐암)등에 대한 in vitro에서의 세포독성을 실험하였다. 그 결과, acacetin (2)이 HCT116 $(IC_{50}\;2.44\;{\mu}g/ml)$과 UO-31 $(IC_{50}\;2.89\;{\mu}g/ml)$에서 유의할만한 세포독성을 나타내었다.

Two known flavonoids, luteolin (1) and acacetin (2) were isolated from a $CHCI_3$ soluble fraction of the whole plants of Chrysanthemum zawadskii Herbich var. latilobum Kitamura, and their structures were determined by NMR analysis. The luteolin (1) was isolated from this plant for the first time. These compounds were examined for their in vitro cytotoxic activities against four human cancer cell lines including HCT116 (colon), UO-31 (renal), PC-3 (prostate) and A549 (lung) by sulforhodamine B(SRB) assay. Acacetin (2) showed significant cytotoxic activity against HCT116 and UO-31 cells with an $IC_{50}$ of 2.44 and $2.89\;{\mu}g/ml$, respectively.

키워드

참고문헌

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  8. Anti-inflammatory activity of Chrysanthemum zawadskii var. latilobum leaf extract through haem oxygenase-1 induction vol.4, pp.2, 2012, https://doi.org/10.1016/j.jff.2012.02.007