YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Isolation and Carbohydrate Binding Specificity of a Lectin from the Hemolymph of Coastal Crab Macrophthalmus Japonicus

해양칠게 혈림프로부터 렉틴의 분리 및 당 결합특성

  • Published : 2006.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

A calcium-dependent sialic acid-binding lectin has been isolated by thyroglobulin-affinity chromatography from the coastal crab Macrophthalmus Japonicus. This lectin, Macrophthalmus Japonicus lectin (MJL), was eluted with 50mM Tris-HCl, 0.3 M NaCl, 10 mM EDTA, and the recovery yield from the crude protein extract was about 5.6%. The molecular weight of MJL was estimated as 65 kDa in SDS-PAGE both under reducing and non-reducing conditions. MJL induced an agglutination reaction in rabbit, rat, and mouse erythrocytes, but not in human ABO types. This activity was effectively inhibited by sialoglycoproteins such as fetuin, bovine submaxillary mucin, and thyroglobulin.

Keywords

References

  1. Lis, H. and Sharon, N. : Lectins as molecules and as tools. Annu. Rev. Biochem. 55, 35 (1986) https://doi.org/10.1146/annurev.bi.55.070186.000343
  2. Sharon, N. : Lectin-carbohydrate complexes of plants and animals: an atomic view. Trends in Biochem. Sciences 18, 221 (1993) https://doi.org/10.1016/0968-0004(93)90193-Q
  3. Cummings, R. D. : Use of lectins in analysis of glycoconjugates. Methods in Enzymology 230, 66 (1994) https://doi.org/10.1016/0076-6879(94)30008-9
  4. Kocaurek, J. : The Lectins; Properties, function, and applications in biology and medicine (Leiner, I. E., Sharon, N. and Goldstein, I. J.). Academic Press p.1 (1986)
  5. Schauer, R. : Sialic Acids; Chemistry, Metabolism and Function, Springer-Verlag, New York, p.323 (1982)
  6. Higashi, H., Hirabayashi, Y., Fukui, Y., Naiki, M., Matsumoto, M., Ueda, S. and Kato, S. : Characterization of Nglycolylneuraminic acid-containing gangliosides as tumorassociated Hanganutziu-Deicher antigen in human colon cancer. Cancer Res. 45, 3796 (1985)
  7. Cheresh, D. A., Reisfeld, R. A. and Varki, A. P. : O-acetylation of disialoganglioside GD3 by human melanoma cells creates a unique antigenic determinant. Science 225, 844 (1984) https://doi.org/10.1126/science.6206564
  8. Cheng, T. C. : Evolution of receptors. Comp. Pathol. 5, 33 (1984)
  9. Ravindranath, M. H., Paulson, J. C. and Irie, R. F. : Human melanoma antigen O-acetylated ganglioside GD3 is recognized by Cancer antennarius lectin. J. Biol. Chem. 260, 8838 (1985)
  10. Kawai, T., Kato, A., Higashi, H., Kato, S. and Naiki, M. : Quantitative determination of N-glycolylneuraminic acid expression in human cancerous tissues and avian lymphoma cell lines as tumor-associated sialic acid by gas chromatography mass spectrometry. Cancer Res. 51, 1242 (1991)
  11. Kim, B. S., Oh, K. T., Cho, D. H., Kim, Y. J., Kong, K. H. and Kim, H. H. : A sialic acid-binding lectin from the legume Maackia fauriei: comparison with lectins from M. amurensis. Plant Sci. 167, 1315 (2004) https://doi.org/10.1016/j.plantsci.2004.06.029
  12. Park, J. H., Ryu, C. S., Kim, H. N., Na, Y. J., Park, H. J. and Kim, H. H. : A sialic acid-specific lectin from the mushroom Paecilomyces Japonica that exhibits hemagglutination activity and cytotoxicity. Protein Pept Lett. 11, 563 (2004) https://doi.org/10.2174/0929866043406445
  13. Bhavanandan, V. P. and Katlic, A. W. : The interaction of wheat germ agglutinin with sialoglycoproteins. The role of sialic acid. J. Biol. Chem. 254, 4000 (1979)
  14. Mandal, C. and Mandal, C. : Sialic acid binding lectins. Experientia. 46, 433 (1990) https://doi.org/10.1007/BF01954221
  15. Charland, N., Kellens, J. T., Caya, F. and Gottschalk, M. : Agglutination of Streptococcus suis by sialic acid-binding lectins. J. CLin. Microbiol. 33, 2220 (1995)
  16. Vasta, G. R., Warr, G. W. and Marchalonis, J. J. : Serological characterization of humoral lectins from the freshwater prawn Macrobrachium rosenbergii. Dev. Comp. Immunol. 7, 13 (1983) https://doi.org/10.1016/0145-305X(83)90050-2
  17. Ravindranath, M. H., Higa, H. H., Cooper, E. L. and Paulson, J. C. : Purification and characterization of an O-acetylsialic acid-specific lectin from a marine crab Cancer antennarius. J. Biol. Chem. 260, 8850 (1985)
  18. Mercy, Sr. P. D. and Ravindranath, M. H. : An agglutinin with unique specificity for N-glycolylneuraminic acid residues of thyroglobulin in the hemolymph of a marine crab Scylla serrata (Forskal). Experientia. 48, 498 (1992) https://doi.org/10.1007/BF01928173
  19. Cheng, W., Liu, C. H., Tsai, C. H. and Chen, J. C. : Molecular cloning and characterisation of a pattern recognition molecule, lipopolysaccharide- and beta-1,3-glucan binding protein (LGBP) from the white shrimp Litopenaeus vannamei. Fish Shellfish Immunol. 18, 297 (2005) https://doi.org/10.1016/j.fsi.2004.08.002
  20. Laemmli U. K. : Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680 (1970) https://doi.org/10.1038/227680a0
  21. Bradford, M. : A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 72, 248 (1976) https://doi.org/10.1016/0003-2697(76)90527-3
  22. Maghil E. P. D., Mercy P., Renuka B. N and Jeya S. S : Purification and characterization of a sialic acid specific lectin from the hemolymph of the freshwater crab Paratelphusa jacquemontii. Eur. J. Biochem. 270, 4348 (2003) https://doi.org/10.1046/j.1432-1033.2003.03828.x
  23. Ahmad, S., Khan, R. H. and Ahmad, A. : Physicochemical characterization of Cajanus cajan lectin: effect of pH and metal ions on lectin carbohydrate interaction. Biochim. Biophys Acta. 1427, 378 (1999) https://doi.org/10.1016/S0304-4165(99)00035-5
  24. Lim, S. B., Chua, C. T. and Hashim, O. H. : Isolation of a mannose-binding and IgE- and IgM-reactive lectin from the seeds of Artocarpus integer. J. Immunol. Methods 209, 177 (1997) https://doi.org/10.1016/S0022-1759(97)00158-0
  25. Warren, L. : The distribution of sialic acids in nature. Comp. Biochem. Physiol. 10, 153 (1963) https://doi.org/10.1016/0010-406X(63)90238-X
  26. Segler, K., Rahmann, H. and Rosner, H. : Chemotaxonomical investigations on the occurrence of sialic acids in protostomia and deuterostomia. Biochem. Syst. Ecol. 6, 87 (1978) https://doi.org/10.1016/0305-1978(78)90030-3