The Journal of Internal Korean Medicine (대한한방내과학회지)

The Society of Internal Korean Medicine (대한한방내과학회)
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The Effect of Daecheongryong-tang and prescription A on airway mucin secretion

Mucin 분비에 미치는 대청룡탕(大靑龍湯) 및 <석실비록(石室秘錄)> 급치법방(急治法方)에 대한 연구

  • Park, Wan-Yeol (Deptartment of Internal Medicine, College of Oriental Medicine, Dongguk University) ;
  • Suh, Woon-Gyo (Deptartment of Internal Medicine, College of Oriental Medicine, Dongguk University)
  • 박완열 (동국대학교 한의과대학 폐계내과학교실) ;
  • 서운교 (동국대학교 한의과대학 폐계내과학교실)
  • Published : 2006.03.30
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Abstract

Objectives : This study was done with intend to investigate whether two oriental medical prescriptions, daecheongryong-tang (DCRT) and prescription A (P-A) significantly affect mucin release from cultured hamster tracheal surface epithelial (HTSE) cells. Methods : Confluent HTSE cells were metabolically radiolabeled with $^3H$-glucosamine for 24 hrs and chased for 30 min in the presence of DCRT or P-A to assess the effect of each agent on $^3H$-mucin release. Possible cytotoxicities of each agent were assessed by measuring lactate dehydrogenase (LDH) release. Also, the effects of DCRT and P-A on contractility of isolated tracheal smooth muscle were investigated. Results were as follows : 1. DCRT significantly inhibited mucin release from cultured HTSE cells, with significant cytotoxicity. 2. P-A significantly increased mucin release from cultured HTSE cells, with significant cytotoxicity. 3. DCRT inhibited Ach-induced contraction of isolated tracheal smooth muscle. 4. P-A also inhibited Ach-induced contraction of isolated tracheal smooth muscle. Conclusion: Results suggest that DCRT and P-A have regulating effects on mucin secretion from goblet cells. Further investigation is needed, because of the value in finding novel agents to this purpose, and these oriental medical prescriptions have potential for this role.

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