Journal of Pharmacopuncture (대한약침학회지)

KOREAN PHARMACOPUNCTURE INSTITUTE (대한약침학회)
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Effects of Cultivated Wild Ginseng Herbal Acupuncture to the serum cytokine on Hepatic Metastatic Model using Colon26-L5 Carcinoma Cells

Colon26-L5 대장암 세포를 이용한 간전이 모델에 산삼약침 처치가 혈중 cytokine에 미치는 영향

  • Cho, Byung-Jun (Department of Acupuncture & Moxibustion, Oriental Medical College, Sangji University) ;
  • Kwon, Ki-Rok (Department of Acupuncture & Moxibustion, Oriental Medical College, Sangji University)
  • 조병준 (상지대학교 한의과대학 침구학 교실) ;
  • 권기록 (상지대학교 한의과대학 침구학 교실)
  • Published : 2006.02.28
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Abstract

Objective : This experiment was conducted to evaluate inhibitory effects against hepatic metastasis by cultivated wild ginseng Herbal Acupuncture. Methods : Colon26-L5 carcinoma cells were injected through hepatic portal vein to induce hepatic metastatic cancer. After treated cultivated wild ginseng Herbal Acupuncture and investigated various kinds of cytokine level using cytokine chip. Results : 1. Mice treated with cultivated wild ginseng Herbal Acupuncture reduced the level of $IL-l{\alpha}$, $IL-{\beta}$, and $TNF-{\alpha}$ compared to the control group. 2. Mice treated with cultivated wild ginseng Herbal Acupuncture was not showed significant change in the level of IL-4, IL-l0, IL-12 and $INF-{\gamma}$ compared to the control group. 3. Observing the level of various kinds of cytokine, cultivated wild ginseng Herbal Acupuncture was suppressed pro-inflammatory cytokine. These findings indicate cultivated wild ginseng Herbal Acupuncture is possible to use the inflammatory disease and futher studies carry out for the explanation of anticancer mechanism.

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References

  1. 대한약침학회. 약침요법 시술 지침서. 서울: 대한약침학회. 1999 ; 13-14, 112-118, 138-203.
  2. 손 인철 외. 약침요법 . 일중사. 1999 ; 17-18.
  3. Ministary of health and welfare. national nutrition survey report. 2003.
  4. 류재현, 김광호, 심강섭, 박응범, 서정수. 대장직장암의 전이성 간암에 대한 항암색전술. 대한외과학회지 2000;58(1):109–114.
  5. Cascino S, Walder S. Chemo-embolization in the treatment of liver metastases from colorectal cancer. Cancer Treatment Reviews 1996;22:355. https://doi.org/10.1016/S0305-7372(96)90008-0
  6. Isenberg J, Fischbach R, Lruger I, Keller HW. Treatment of liver metastases from colorectal cancer. Anticancer Reserch 1996;16:1291.
  7. 선병호, 안병권, 김병수, 김만기, 김지헌. 원발성 및 전이성 간암에 대한 외과적 항암 Lipiodol 색전술. 대한외과학회지 1996;50(10):47.
  8. 권기록, 조아라, 이선구. 정맥주입용 산양산삼 증류약침의 급성, 아급성 독성 실험 및 Sarcoma-180 항암효과에 관한 실험적 연구. 대한약침학회지 2003;6(2):7–27. https://doi.org/10.3831/KPI.2003.6.2.007
  9. 권기록. 정맥주입용 산삼약침이 인체에 미치는 영향에 관한 임상적 연구. 대한약침학회지 2004;7(1):15–26. https://doi.org/10.3831/KPI.2004.7.1.015
  10. 곡경승, 이선구, 권기록. pH 및 전해질 조절 산양산삼 증류약침의 apoptosis에 관한 실험적 연구. 대한침구학회지 2004;21(6):1–17.
  11. Kwon Ki-Rok; Park Chi-Wan; Ra Min-Soo; Cho Chong-Kwan. Clinical Observation of Multiple Metastatic Cancer Patient with Hepatocellular Carcinoma treated with Cultivated Wild Ginseng Herbal Acupuncture Therapy. J. of Korean Acupuncture & Moxibustion Society 2005;22(2):211–217.
  12. Ohnishi Y, Sakamoto T, Fujii H et al. Characterization of a liver metastatic variant of murine colon 26 carcinoma cells. Tumour Biol 1997;18:112–122.
  13. David R, Goldman. Complete Home Medical Guide. DK publishing, INC 2003:274–277.
  14. 單書健. 神農本草經校證, 吉林科學技術出版社. 1988 ; 150.
  15. 김성진, 신순식, 서부일, 지선영. 산삼, 장뇌삼, 인삼의 항암효과에 대한 비교연구. 대한본초학회지 2004;19(2):41–50.
  16. Yun T. K., Choi S. Y. Cancer Epidemiol. Biomarkers Prevent 1995;4:401.
  17. 서성옥, 정철현, 손길수. The Ginseng Review. 25. 1998 ; 10.
  18. 곽이성, 김영숙, 신한재, 송용범, 박종대. 홍삼 산성다당체(RGAP)와 항암제의 병행투여에 의한 항암시너지 효과. J. Ginseng Res 2003;27(2):47–51. https://doi.org/10.5142/JGR.2003.27.2.047
  19. 김세종. 면역학 길라잡이. 서울: 고려의학. 2000 ; 15-17.
  20. Paul WE, Seder RA. Lymphocyte responses and cytokines. Cell 1994;76:241–251. https://doi.org/10.1016/0092-8674(94)90332-8
  21. Constant SL, Bottomly K. Induction Th 1 and Th 2 CD4+ T cell response, The alternative approaches. Annu. Rev. Immunol 1997;114:297–322. https://doi.org/10.1146/annurev.immunol.15.1.297
  22. Sell S. Cell mediated immunity in vitro in immunology, immunopathology and immunity. Hergestown, Maryland; Herpers & Row Pub 1980:144–171.
  23. Romgnani S. Human Th1 and Th2 subsets : doubt no more. Immuol. Today 1991;12:256–257. https://doi.org/10.1016/0167-5699(91)90120-I
  24. Zurawski G, De Vries J.E. Interlukin 13, an interlukin 4-like cytokine that acts on monocytes and B cells, but not on T cells. Immunol. Today 1994;15:19–26. https://doi.org/10.1016/0167-5699(94)90021-3
  25. Schluger NW, Rom WN. The host immune response totuberculosis. Am J Respir Crit Care Med 1998;157:679–691. https://doi.org/10.1164/ajrccm.157.3.9708002
  26. Brown M. A., Hural J. Functions of IL-4 and control of its expression. Crit. Rev. Immunol 1997;17:1–32. https://doi.org/10.1615/CritRevImmunol.v17.i1.10
  27. Fiorentino DF, Zlotnik A, Mosmann TR, Howard M, O'Garra A. IL-10 inhibits cytokine production by activated marcrophages. J Immunol 1991;147:3815–22.
  28. Kobayashi M, Fitz L, Ryan M, Hewick RM, Clark SC, Chan S, Louden R, Sherman F, et al. Identification and purification of natural killer cell stimulatory factor (NKSF), a cytokine with multiple biologic effects on human lymphocytes. J Exp Med 1989;170:827. https://doi.org/10.1084/jem.170.3.827
  29. Samuel C. E. Antiviral actions of interferons. Clin. Microbiol. Rev 2001;14:778–809. https://doi.org/10.1128/CMR.14.4.778-809.2001

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