The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Inhibition of gene expression and production of iNOS and $TNF-{\alpha}$ in LPS-stimulated microglia by On-Bi-Tang

오령산가온비탕의 BV2에서 LPS로 유도된 nitric oxide 및 $TNF-{\alpha}$ 생성에 미치는 영향

  • Son, Hye-Young (Department of Herbology, Internal Medicine, College of Oriental Medicine, Dongguk University) ;
  • Kim, Sang-Woon (Department of Herbology, Internal Medicine, College of Oriental Medicine, Dongguk University) ;
  • Jung, Sun-Ju (Department of Herbology, Internal Medicine, College of Oriental Medicine, Dongguk University) ;
  • Jung, Hyo-Won (Department of Herbology, Internal Medicine, College of Oriental Medicine, Dongguk University) ;
  • Yoon, Cheol-Ho (Department of Herbology, Internal Medicine, College of Oriental Medicine, Dongguk University) ;
  • Park, Yong-Ki (Department of Herbology, Internal Medicine, College of Oriental Medicine, Dongguk University)
  • 손혜영 (동국대학교 한의과대학 본초학교실) ;
  • 김상운 (동국대학교 한의과대학 본초학교실) ;
  • 정선주 (동국대학교 한의과대학 본초학교실) ;
  • 정효원 (동국대학교 한의과대학 본초학교실) ;
  • 윤철호 (동국대학교 한의과대학 본초학교실) ;
  • 박용기 (동국대학교 한의과대학 본초학교실)
  • Published : 2006.06.30
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Abstract

Objectives : On-Bi-Tang(OB) has been prescribed Chinese traditional medicine for the treatment of inflammatory disease such as chronic renal failure. In this study, we investigated the anti-inflammatory effect of OB extract in the BV2 murine microglial cells. Methods : After the water extract of OB was treated in BV2 cells, murine microglial line, the cell viability was measured by MTT assay. The production of nitric oxide (NO) and $TNF-{\alpha}$ was determined based on Griess reagent and enzyme linked immunosorbant assay (ELISA). mRNA expression of inducible nitric oxide synthase (iNOS) and $TNF-{\alpha}$ was analyzed by RT-PCR. Results : OB extract significantly inhibited the LPS-induced production of NO and TNF-a in BV2 cells. OB extract also suppressed the mRNA expression of iNOS and $TNF-{\alpha}$ in BV2 cells activated with LPS. Conclusion : These data suggests that OB extract may have the anti-inflammatory effect through the modulation of NO production and inflammatory cytokine such as $TNF-{\alpha}$.

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