Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Effects of Traditional Wine by using Mycelium of Phellinus Iinteus on the Expression of Inflammation-Related Proteins in HepG2 Cells

상황버섯 균사체를 이용한 전통주의 추출물이 HepG2 세포의 염증관련 단백질 발현에 미치는 영향

  • Lee, Su-Jin (Departments of Anatomy, College of Oriental Medicine, Dong-Eui University) ;
  • Han, Min-Ho (Department of Biochemistry, College of Oriental Medicine, Dong-Eui University) ;
  • Lee, Yong-Tae (Department of Physiology, College of Oriental Medicine, Dong-Eui University) ;
  • Huh, Man-Kyu (Department of Molecular Biology, College of Natural Science, Dong-Eui University) ;
  • Chung, Kyung-Tae (Department of Life Science and Biotechnology, College of Natural Science, Dong-Eui University) ;
  • Jeong, Young-Kee (Department of Biotechnology, College of Natural Resources and Life Science, Dong-A University) ;
  • Choi, Yung-Hyun (Department of Biochemistry, College of Oriental Medicine, Dong-Eui University) ;
  • Choi, Byung-Tae (Departments of Anatomy, College of Oriental Medicine, Dong-Eui University)
  • 이수진 (동의대학교 한의과대학 해부학교실) ;
  • 한민호 (동의대학교 한의과대학 생화학교실) ;
  • 이용태 (동의대학교 한의과대학 생리학교실) ;
  • 허만규 (동의대학교 자연과학대학 분자생물학과) ;
  • 정경태 (동의대학교 자연과학대학 생명응용과학과) ;
  • 정영기 (동아대학교 생명자원과학대학 생명공학과) ;
  • 최영현 (동의대학교 한의과대학 생화학교실) ;
  • 최병태 (동의대학교 한의과대학 해부학교실)
  • Published : 2006.08.25
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Abstract

It was examined that the effect of fermented traditional wine made by using mycelium of Phellinus linteus on the expression of inflammation-related proteins in HepG2 cells. HepG2 cells were incubated with or without ertract of traditional wine (ETMP), then analyzed by microscopic observation, reverse transcription polymerase chain reaction (RT-PCR) and Western blot. The results of RT-PCR and Western blot analyses showed that the level of inducible nitric oxide synthase (iNOS), cyclooxygenase (COX)-2 and tumor necrosis factor $(TNF)-{\alpha}$ was induced by LPS, Dut the treatment of ETMP inhibited the expression of these proteins and its mRNAs. Besides, the results of Western blot analyses showed that the expression of nuclear $factor-{\kappa}Bp65$ and $inhibitory-{\kappa}B{\alpha}$ were also slightly affected by ETMP treatment. These results suggest that ETM P alleviate the expression of inflammation-related protein expressions and thus may be used as a functional alcoholic beverage.

Keywords

References

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