Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Effect of Thesium Chinense Turczaninow on Breast Cancer Chemopreventive enzyme activity in In vitro

In vitro에서의 댑싸리하고초의 유방암예방효소 활성에 미치는 영향

  • Shon, Yun-Hee (Intractable Diseases Research Center, College of Medicine, Dongguk University) ;
  • Kim, Mee-Kyung (Intractable Diseases Research Center, College of Medicine, Dongguk University) ;
  • Park, Sun-Dong (Cardiovascular Medical Research Center and Department of Pharmacology, College of Medicine, Dongguk University) ;
  • Nam, Kyung-Soo (Cardiovascular Medical Research Center and Department of Pharmacology, College of Medicine, Dongguk University)
  • 손윤희 (동국대학교 난치병한양방치료연구센터) ;
  • 김미경 (동국대학교 난치병한양방치료연구센터) ;
  • 박선동 (심혈관계질환 천연물개발연구센터 및 외과대학 약리학교실) ;
  • 남경수 (심혈관계질환 천연물개발연구센터 및 의과대학 약리학교실)
  • Published : 2006.06.25
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Abstract

The effect of water extract from Thesium chinese Turczaninow (TCTW) on proliferation of human breast cancer cells, nitric oxide production, nitric oxide synthase expression, and ornithine decarboxylase activity was tested. TCTW inhibited the growth of both estrogen-independent MDA-MB-231 and estrogen-dependent MCF-7 human breast cancer cells. Lipopolysaccharide-induced nitric oxide (NO) production was significantly reduced by TCTW at the concentrations of 1.0 (p<0.05) and 5.0 mg/ml (p<0.005). Expression of inducible nitric oxide synthase (iNOS) was also suppressed with the treatment of TCTW in Western blot analysis. TCTW inhibited induction of ornithine decarboxylase by 12-O-tetradecanoylphorbol-13-acetate (TPA), a key enzyme of polyamine biosynthesis, which is enhanced in tumor promotion. Therefore, TCTW is worth further investigation with respect to breast cancer chernoprevention or therapy.

Keywords

References

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