Apoptosis Induced by n-butanol Fraction of DoJeokSeungKi-Tang Extracts in U-937 Cells

U-937 세포에서 도적승기탕(導赤承氣湯) 추출물 중 부탄올 분획에 의한 Apoptosis 유도

  • Park, Pyeong-Beom (Department of Pathology, College of Oriental Medicine, Woosuk University) ;
  • Jeong, Han-Sol (Department of Pathology, College of Oriental Medicine, Woosuk University) ;
  • Kim, Ho (Department of Pathology, College of Oriental Medicine, Woosuk University) ;
  • Jin, Jae-Ho (Department of Pathology, College of Oriental Medicine, Woosuk University) ;
  • Jeong, Sang-Hun (Department of Pathology, College of Oriental Medicine, Woosuk University) ;
  • Han, Ung (Department of Pathology, College of Oriental Medicine, Woosuk University) ;
  • Lee, Moon-Won (Department of Anatomy, College of Oriental Medicine, Woosuk University) ;
  • Lee, Kwang-Gyu (Department of Pathology, College of Oriental Medicine, Woosuk University)
  • 박평범 (우석대학교 한의과대학 병리학교실) ;
  • 정한솔 (우석대학교 한의과대학 병리학교실) ;
  • 김호 (우석대학교 한의과대학 병리학교실) ;
  • 진재호 (우석대학교 한의과대학 병리학교실) ;
  • 정상훈 (우석대학교 한의과대학 병리학교실) ;
  • 한웅 (우석대학교 한의과대학 병리학교실) ;
  • 이문원 (우석대학교 한의과대학 해부학교실) ;
  • 이광규 (우석대학교 한의과대학 병리학교실)
  • Published : 2006.06.25

Abstract

To investigate the anti-cancer effects of n-butanol fraction of DoJeokSeungKi-Tang extracts(nBFD) in U-937 cells. MTT assay was used to determine U-937 cells proliferation. Flow cytometry was used to detect apoptosis. Bcl-xl anti-apoptotic protein and caspase-3, p53 pro-apoptotic protein were examined by Western blot analysis. nBFD inhibited the proliferation of U-937 cells in a dose-dependent manner. The cells treated with nBFD showed a typical apoptotic process by increasing sub-Gl peak. nBFD reduced uptake of 3,3'dihexyloxacarbocyanine iodide(DiOC6) a fluorochrome which incorporates into cells dependent upon their mitochondrial transmembrane potential$({\triangle}{\psi}m)$. nBFD induced in U-937 cells apoptosis mainly via increasing sub-Gl peak, regulation of Bcl-xl, caspase-3 and p53 protein.

Keywords

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