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Apoptosis Induced by n-butanol Fraction of DoJeokSeungKi-Tang Extracts in U-937 Cells  

Park, Pyeong-Beom (Department of Pathology, College of Oriental Medicine, Woosuk University)
Jeong, Han-Sol (Department of Pathology, College of Oriental Medicine, Woosuk University)
Kim, Ho (Department of Pathology, College of Oriental Medicine, Woosuk University)
Jin, Jae-Ho (Department of Pathology, College of Oriental Medicine, Woosuk University)
Jeong, Sang-Hun (Department of Pathology, College of Oriental Medicine, Woosuk University)
Han, Ung (Department of Pathology, College of Oriental Medicine, Woosuk University)
Lee, Moon-Won (Department of Anatomy, College of Oriental Medicine, Woosuk University)
Lee, Kwang-Gyu (Department of Pathology, College of Oriental Medicine, Woosuk University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.20, no.3, 2006 , pp. 590-595 More about this Journal
Abstract
To investigate the anti-cancer effects of n-butanol fraction of DoJeokSeungKi-Tang extracts(nBFD) in U-937 cells. MTT assay was used to determine U-937 cells proliferation. Flow cytometry was used to detect apoptosis. Bcl-xl anti-apoptotic protein and caspase-3, p53 pro-apoptotic protein were examined by Western blot analysis. nBFD inhibited the proliferation of U-937 cells in a dose-dependent manner. The cells treated with nBFD showed a typical apoptotic process by increasing sub-Gl peak. nBFD reduced uptake of 3,3'dihexyloxacarbocyanine iodide(DiOC6) a fluorochrome which incorporates into cells dependent upon their mitochondrial transmembrane potential$({\triangle}{\psi}m)$. nBFD induced in U-937 cells apoptosis mainly via increasing sub-Gl peak, regulation of Bcl-xl, caspase-3 and p53 protein.
Keywords
mitochondrial transmenbrane potential; RT-PCR;
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