Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Effect of Rhizoma Zingiberis Siccatum Extract on the Induction of HL-60 Human Leukemia Cell Differentiation

HL-60 세포에 대한 건강의 분화유도 효과

  • Oh, Yu-Mi (Division of Natural Science, College of Natural Sciences, Wonkwang University) ;
  • Ju, Seong-Min (Department of Pathology, College of College of Oriental Medicine, Wonkwang University) ;
  • Kim, Young-Mi (Division of Natural Science, College of Natural Sciences, Wonkwang University) ;
  • Yun, Yong-Gab (Department of Prescription, College of Oriental Medicine, Wonkwang University) ;
  • Jeon, Byung-Hun (Department of Pathology, College of College of Oriental Medicine, Wonkwang University) ;
  • Kim, Won-Sin (Division of Natural Science, College of Natural Sciences, Wonkwang University)
  • 오유미 (원광대학교 자연과학대학 생명과학부) ;
  • 주성민 (원광대학교 한의과대학 병리학교실) ;
  • 김영미 (원광대학교 자연과학대학 생명과학부) ;
  • 윤용갑 (원광대학교 한의과대학 방제학교실) ;
  • 전병훈 (원광대학교 한의과대학 병리학교실) ;
  • 김원신 (원광대학교 자연과학대학 생명과학부)
  • Published : 2006.04.25
Download PDF
⟨ Previous Next ⟩

Abstract

We have examined the induction of HL-60 cell differentiation by treatment of the water extract of Rhizoma Zingiberis Siccatum, which is a Korean traditional herbal medicine. It was observed that HL-60 cell proliferation was dose-dependently inhibited by treatment with various dose of Rhizoma Zingiberis Siccatum extract. The level of inhibition was identical to those of ATRA-treated cells. Rhizoma Zingiberis Siccatum extract treatment caused a significant change in NBT reduction (47%) and enhanced ATRA-induced NBT reduction. Treatment of Rhizoma Zingiberis Siccatum to HL-60 cells increased only CD11 b expression in the cells, and also increased markedly G0/G1 stage arrest. This can suggest that Rhizoma Zingiberis Siccatum induced the differentiation of HL-60 cell and enhanced ATRA-induced differentiation predominantly along the granulocytic lineage. The results present here show that Rhizoma Zingiberis Siccatum extract contains the potential effect of differentiation induction or acts as a agent enhancing the induction of differentiation.

Keywords

References

  1. Bestilny, L.J., Riabowol, K.T. A role for serine proteases in mediating phorbol ester-induced differentiation of HL-60 cells. Exp. Cell Res. 256, 264-271, 2000 https://doi.org/10.1006/excr.2000.4821
  2. Breitman, T.R., Selonick, S.E., Collins, S.J. Induction of differentiation of the human promyelocytic leukemia cell line (HL-60) by retinoic acid. Proc. Natl. Acad. Sci. USA. 77, 2936-2940, 1980
  3. Brown, G., Drayson, M.T., Durham, J., Toellner, K.M., Hughes, P.J., Choudhry, M.A., Taylor, D.R., Bird, R., Michell, R.H. HL60 cells halted in G1 or S phase differentiate normally. Exp. Cell. Res. 281, 28-38, 2002 https://doi.org/10.1006/excr.2002.5654
  4. Camarasa, M.V., Castro-Galache M.D., Carrasco-Garcia, E., Garcia-Morales, P., Saceda, M., Ferragut, J.A. Differentiation and drug resistance relationships in leukemia cells. J. Cell Biochem. 94, 98-108, 2005 https://doi.org/10.1002/jcb.20278
  5. Chung, T.W., Choi, H.J., Lee, Y.C., Kim, C.H. Molecular mechanism for transcriptional activation of ganglioside GM3 synthase and its function in differentiation of HL-60 cells. Glycobiology. 15, 233-244, 2005 https://doi.org/10.1093/glycob/cwh156
  6. Collins, S.J. The HL-60 promyelocytic leukemia cell line: proliferation, differentiation, and cellular oncogene expression. Blood. 70, 1233-1244, 1987
  7. Datto, M.B., Li, Y., Panus, J.F., Howe, D.J., Xiong, Y., Wang, X.F. Transforming growth factor beta induces the cyclin-dependent kinase inhibitor p21 through a p53-independent mechanism. Proc. Natl. Acad. Sci. USA. 92, 5545-5549, 1995
  8. Degos, L., Dombret, H., Chomienne, C., Daniel, M.T., Miclea, J.M., Chastang, C., Castaigne, S., Fenaux, P. All-trans-retinoic acid as a differentiating agent in the treatment of acute promyelocytic leukemia. Blood. 85, 2643-2653, 1995
  9. Drayson, M.T., Michell, R.H., Durham, J., Brown, G. Cell proliferation and CD11b expression are controlled independently during HL60 cell differentiation initiated by 1,25 alpha-dihydroxyvitamin D(3) or all-trans-retinoic acid. Exp. Cell Res. 266, 126-134, 2001 https://doi.org/10.1006/excr.2001.5200
  10. Gianni, M., Ponzanelli, I., Mologni, L., Reichert, U., Rambaldi, A., Terao, M., Garattini, E. Retinoid-dependent growth inhibition, differentiation and apoptosis in acute promyelocytic leukemia cells. Expression and activation of caspases. Cell Death Differ. 7, 447-460, 2000 https://doi.org/10.1038/sj.cdd.4400673
  11. Hickstein, D.D., Back, A.L., Collins, S.J. Regulation of expression of the CD11b and CD18 subunits of the neutrophil adherence receptor during human myeloid differentiation. J. Biol. Chem. 264, 21812-21817, 1987
  12. Hickstein, D.D., Baker, D.M., Gollahon, K.A., Back, A.L. Identification of the promoter of the myelomonocytic leukocyte integrin CD11b. Proc. Natl. Acad. Sci. USA. 89, 2105-2109, 1992
  13. Horie, N., Mori, T., Asada, H., Ishikawa, A., Johnston, P. G., Takeishi, K. Implication of CDK inhibitors p21 and p27 in the differentiation of HL-60 cells. Biol. Pharm. Bull. 27, 992-997, 2004 https://doi.org/10.1248/bpb.27.992
  14. Huang, W.W., Yang, J.S., Lin, C.F., Ho, W.J., Lee, M.R. Pycnogenol induces differentiation and apoptosis in human promyeloid leukemia HL-60 cells. Leuk. Res. 29, 685-692, 2005 https://doi.org/10.1016/j.leukres.2004.10.006
  15. James, S.Y., Williams, M.A., Kelsey, S.M., Newland, A.C., Colston, K.W. The role of vitamin D derivatives and retinoids in the differentiation of human leukaemia cells. Biochem. Pharmacol. 54, 625-634, 1997 https://doi.org/10.1016/S0006-2952(97)00195-0
  16. Kong, G., Lee, S.J., Kim, H.J., Surh, Y.J., Kim, N.D. Induction of granulocytic differentiation in acute promyelocytic leukemia cells (HL-60) by 2-(allylthio) pyrazine. Cancer Lett. 144, 1-8, 1999 https://doi.org/10.1016/S0304-3835(99)00115-9
  17. Lee, K.H., Chang, M.Y., Ahn, J.I., Yu, D.H., Jung, S.S., Choi, J.H. Differential gene expression in retinoic acid-induced differentiation of acute promyelocytic leukemia cells, NB4 and HL-60 cells. Biochem. Biophys. Res. Commun. 296, 1125-1133, 2002 https://doi.org/10.1016/S0006-291X(02)02043-0
  18. Makishima, M., Umesono, K., Shudo, K., Naoe, T., Kishi, K., Honma, Y. Induction of differentiation in acute promyelocytic leukemia cells by 9-cis retinoic acid alpha-tocopherol ester (9-cis tretinoin tocoferil). Blood. 91, 4715-4726, 1998
  19. Mishima, S., Inoh, Y., Narita, Y., Ohta, S., Sakamoto, T., Araki, Y., Suzuki, K.M., Akao, Y., Nozawa, Y. Identification of caffeoylquinic acid derivatives from Brazilian propolis as constituents involved in induction of granulocytic differentiation of HL-60 cells. Bioorg. Med. Chem. 13, 5814-5818, 1995 https://doi.org/10.1016/j.bmc.2005.05.044
  20. Newburger, P.E., Chovaniec, M.E., Greenberger, J.S., Cohen, H.J. Functional changes in human leukemic cell line HL-60. A model for myeloid differentiation. J. Cell Biol. 82, 315-322, 1979 https://doi.org/10.1083/jcb.82.2.315
  21. Noble, J.M., Ford, G.A., Thomas, T.H. Effect of aging on CD11b and CD69 surface expression by vesicular insertion in human polymorphonuclear leucocytes. Clin. Sci. 97, 323-329, 1999 https://doi.org/10.1042/CS19990042
  22. Pae, H.O., Seo, W.G., Kim, N.Y., Oh, G.S., Kim, G.E., Kim, Y.H., Kwak, H.J., Yun, Y.G., Jun, C.D., Chung, H.T. Induction of granulocytic differentiation in acute promyelocytic leukemia cells (HL-60) by water-soluble chitosan oligomer. Leuk. Res. 25, 339-346, 2001 https://doi.org/10.1016/S0145-2126(00)00138-7
  23. Prall, O.W., Sarcevic, B., Musgrove, E.A., Watts, C.K., Sutherland, R.L. Estrogen-induced activation of Cdk4 and Cdk2 during G1-S phase progression is accompanied by increased cyclin D1 expression and decreased cyclin-dependent kinase inhibitor association with cyclin E-Cdk2. J. Biol. Chem. 272, 10882-10894, 1997 https://doi.org/10.1074/jbc.272.16.10882
  24. Roberts, P.J., Khwaja, A., Lie, A.K., Bybee, A., Yong, K., Thomas, N.S., Linch, D.C. Differentiation-linked changes in tyrosine phosphorylation, functional activity, and gene expression downstream from the granulocyte-macrophage colony-stimulating factor receptor. Blood. 84, 1064-1073, 1994
  25. Sachs, L. Constitutive uncoupling of pathways of gene expression that control growth and differentiation in myeloid leukemia: a model for the origin and progression of malignancy. Proc. Natl. Acad. Sci. USA. 77, 6152-6156, 1980
  26. Schwaller, J., Koeffler, H.P., Niklaus, G., Loetscher, P., Nagel, S., Fey, M.F., Tobler, A. Posttranscriptional stabilization underlies p53-independent induction of $p21^{WAF1/CIP1/SDI1}$ in differentiating human leukemic cells. J. Clin. Invest. 95, 973-979, 1995 https://doi.org/10.1172/JCI117806
  27. Tsiftsoglou, A.S., Pappas, I.S., Vizirianakis, I.S. Mechanisms involved in the induced differentiation of leukemia cells. Pharmacol. Ther. 100, 257-290, 2003 https://doi.org/10.1016/j.pharmthera.2003.09.002
  28. Yang, L., Zhao, H., Li, S. W., Ahrens, K., Collins, C., Eckenrode, S., Ruan, Q.G., McIndoe, R.A., She, J. X.Gene expression profiling during all-trans retinoic acid-induced cell differentiation of acute promyelocytic leukemia cells. J. Mol. Diagn. 5, 212-221, 2003 https://doi.org/10.1016/S1525-1578(10)60476-X
  29. hu, B., Zhang, L.H., Zhao, Y.M., Cui, J.R., Strada, S.J. 8-chloroadenosine induced HL-60 cell growth inhibition, differentiation, and G(0)/G(1) arrest involves attenuated cyclin D1 and telomerase and up-regulated $p21^{(WAF1/CIP1)}$. J. Cell Biochem. 2005