Bulletin of the Korean Chemical Society

Korean Chemical Society (대한화학회)
권호 표지
DOI QR코드

DOI QR Code

Temperature-controlled Restrictor for UV Detection in Capillary Supercritical Fluid Chromatography

  • Pyo, Dong-Jin (Department of Chemistry, Kangwon National University)
  • Published : 2006.09.20
Download PDF
⟨ Previous Next ⟩

Abstract

Polyaromatic hydrocarbons were separated by a capillary supercritical fluid chromatographic (SFC) column and detected by a UV detector at the wavelength of 280 $\mu$m. The temperature-controlled restrictor was designed for UV detection. The temperature-controlled restrictor is a 20 cm length of deactivated fused silica of 7 mm i.d. which is held right after UV detector of the capillary SFC. The temperature of the restrictor will control the flow rate of the supercritical carbon dioxide mobile phase through the capillary column in SFC. Thus as the pressure in the column is increased from 1500 psi to 4000 psi during a pressure program, the temperature of 7 $\mu$m fused-silica tube can be varied from 100 to 350 ${^{\circ}C}$ to maintain a constant flow rate.

Keywords

References

  1. Pyo, D.; Shin, H. Anal. Chem. 1999, 71, 4772 https://doi.org/10.1021/ac990440c
  2. Jessop, P. G.; Ikariya, T.; Noyori, R. Science 1995, 269, 1065 https://doi.org/10.1126/science.269.5227.1065
  3. Sako, T.; Sugeta, T.; Otake, K.; Sato, M.; Tsugumi, M.; Hiaki, T.; Hongo, M. J. Chem. Eng. Japan 1997, Vol 30, pp 744-747 https://doi.org/10.1252/jcej.30.744
  4. Pyo, D.; Lim, C. Bull. Korean Chem. Soc. 2005, 26, 312 https://doi.org/10.5012/bkcs.2005.26.2.312
  5. Klesper, E.; Corwin, A. H.; Turner, D. A. J. Org. Chem. 1962, 27, 700 https://doi.org/10.1021/jo01049a069
  6. Kohler, J.; Rose, A.; Schomburg, G. J. High Resolut. Chromatogr. 1988, 11, 191 https://doi.org/10.1002/jhrc.1240110210
  7. Chester, T. L.; Innis, D. P.; Owens, G. D. Anal. Chem. 1985, 57, 2243 https://doi.org/10.1021/ac00289a016
  8. Hawthorne, S. B.; Miller, D. J. J. Chromatogr. 1987, 403, 63 https://doi.org/10.1016/S0021-9673(00)96341-6
  9. Novotny, M.; Springston, S. R.; Peaden, P. A.; Fjeldsted, J. C.; Lee, M. L. Anal. Chem. 1981, 53, 407 https://doi.org/10.1021/ac00226a731
  10. Golay, M. J. E.; Desty, D. H. Gas Chromatography; Academic Press: New York, 1959; p 36
  11. Pyo, D. Analyst 1994, 119, 1315 https://doi.org/10.1039/an9941901315
  12. Berger, T. A.; Toney, C. J. Chromatogr. 1989, 465, 157 https://doi.org/10.1016/S0021-9673(01)92654-8

Cited by

  1. Dynamic control of split flow in packed column supercritical fluid chromatography using dual resistively heated restrictors vol.32, pp.14, 2009, https://doi.org/10.1002/jssc.200900177
  2. Supercritical Fluid Chromatographic Separation of Polyethylene Glycol Polymer vol.29, pp.1, 2008, https://doi.org/10.5012/bkcs.2008.29.1.231
  3. Novel pressure control in supercritical fluid chromatography using a resistively heated restrictor vol.87, pp.3, 2006, https://doi.org/10.1139/v09-005