센서학회지 (Journal of Sensor Science and Technology)

  • 제15권3호
  • /
  • Pages.158-163
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  • 2006
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  • 1225-5475(pISSN)
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  • 2093-7563(eISSN)
한국센서학회 (The Korean Sensors Society)
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강화된 자기장 구배 하에서 나노자성입자를 이용한 미세유체 기반의 면역 측정

Microfluidic immunoassay using superparamagnetic nanoparticles in an enhanced magnetic field gradient

  • 한영기 (한국과학기술원 바이오시스템학과) ;
  • 강주헌 (한국과학기술원 바이오시스템학과) ;
  • 김규성 (한국과학기술원 바이오시스템학과) ;
  • 박제균 (한국과학기술원 바이오시스템학과)
  • 발행 : 2006.05.31
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초록

This paper reports a novel immunoassay method using superparamagnetic nanoparticles and an enhanced magnetic field gradient for the detection of protein in a microfluidic device. We use superparamagnetic nanoparticles as a label and fluorescent polystyrene beads as a solid support. Based on this platform, magnetic force-based microfluidic immunoassay is successfully applied to analyze the concentration of IgG as model analytes. In addition, we present ferromagnetic microstructure connected with a permanent magnet to increase magnetic flux density gradient (dB/dx, ${\sim}10^{4}$ T/m), which makes limit of detection reduced. The detection limit is reduced to about 1 pg/mL.

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참고문헌

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