Journal of Animal Science and Technology

  • 제47권6호
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  • Pages.925-936
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  • 2005
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  • 2672-0191(pISSN)
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  • 2055-0391(eISSN)
한국축산학회 (Korean Society of Animal Sciences and Technology)
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개에서 Pentoxifylline 첨가에 따른 동결정액 성상과 인공수정에 관한 연구

Studies on Frozen Semen Characteristics Following Pentoxifylline Treatment and Artificial Insemination in Dog

  • 지달영 (중앙대학교 동물자원과학과, 중앙대학교 생명환경연구원) ;
  • 김창근 (중앙대학교 동물자원과학과, 중앙대학교 생명환경연구원) ;
  • 이장희 (농촌진흥청 축산연구소) ;
  • 박상재 (농촌진흥청 축산연구소) ;
  • 류일선 (농촌진흥청 축산연구소) ;
  • 류재원 (중앙대학교 동물자원과학과, 중앙대학교 생명환경연구원) ;
  • 이주형 (중앙대학교 동물자원과학과, 중앙대학교 생명환경연구원) ;
  • 정영채 (중앙대학교 동물자원과학과, 중앙대학교 생명환경연구원) ;
  • 방명걸 (중앙대학교 동물자원과학과, 중앙대학교 생명환경연구원)
  • Ji, D.Y. (Department of Animal Science & Technology and BET Research Institute, Chung-Ang University) ;
  • Kim, C.K. (Department of Animal Science & Technology and BET Research Institute, Chung-Ang University) ;
  • Lee, J.H. (National Livestock Research Institute, R.D.A.) ;
  • Park, S.J. (National Livestock Research Institute, R.D.A.) ;
  • Ryu, L.S. (National Livestock Research Institute, R.D.A.) ;
  • Ryu, J.W. (Department of Animal Science & Technology and BET Research Institute, Chung-Ang University) ;
  • Lee, J.H. (Department of Animal Science & Technology and BET Research Institute, Chung-Ang University) ;
  • Jeong, Y.C. (Department of Animal Science & Technology and BET Research Institute, Chung-Ang University) ;
  • Pang, M.G. (Department of Animal Science & Technology and BET Research Institute, Chung-Ang University)
  • 발행 : 2005.12.31
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초록

본 연구는 개 정액 채취방법에 따른 정액성상과 동결전 pentoxifylline의 첨가가 미치는 영향을 조사하였고, CASA를 이용하여 정자의 운동성 측정 및 생존율, 첨체 온전성, 저삼투성 팽창화를 조사하였다. 그리고 자연발정과 발정유기된 암캐에서 동결정액 주입의 임신율을 조사하였다. 1.품종별 정액성상을 조사한 결과 Beagle, English cockers spanie, Collie 및 Grey hound의 정액량과 정자 수에서 큰 차이가 없었으나, Shihtzu에서 정액량과 정자수가 유의적은 아니나 다소 적었다. 2.동결희석액에 pentoxifylline 0, 1 및 3mM 첨가에서 융해 후 정자활력은 각각 46.4, 56.4 및 47.2%였으며 1mM 처리구가 다른 처리구보다 유의적으로 높았다. 3.동결희석액에 pentoxifylline 첨가는 융해 후 정자의 생존성, 첨체막 온전성 및 원형질막 온전성(HOST) 모두 유리한 결과를 나타냈으며, 1mM의 첨가에서 정자의 생존성과 기능성이 향상되었다.4.자연발정에서 발정개시 후 11일, 13일 및 15일째 초음파기로 측정한 난포 크기는 각각 6.5mm, 11.5mm 및 8.0mm이였다. 배란은 13-15일 사이에 일어났다. 5.발정유기한 개체에서 수정후 임신 20일째부터 5일 간격으로 3회 측정한 태낭의 크기는 13.7mm, 28.5mm 및 40.5mm 이었다. 6.자연발정과 발정을 유기한 개체에서 pen- toxifylline 1mM이 첨가된 정액을 동결융해하여 인공수정 후 임신율은 71.4%와 75%로 두 방법간에 차이가 없었으나, 자연발정군에서 평균산자수는 6.6두였고, 발정유군의 평균산자수는 2.6두였다.

The present study evaluated whether pentoxifylline added to the freezing extender could improve the sperm characteristics and function in canine frozen semen. Also the conception rate following AI with frozen-thawed semen was investigated. The beneficial effects of pentoxifylline supplementation were visible in motility, viability, acrosome reaction, and tail swelling patterns. Especially, highest sperm viability and function were obtained in the forzen semen supplemented with 1mM pentoxifylline. The follicle size measured by ultrasonography was 6.48 mm, 11.52 mm and 8.9 mm on 11, 13 and 15 days after the onset of natural estrus, respectively and ovulation occurred on 13 and 15 days. The pregnancy rates in bitches inseminated with frozen semen on natural and induced estrus were 71.4% and 75.0%, respectively. There was no significant difference between the pregnancy rates in bitches inseminated with frozen semen following natural and induced estrus, but the litter size was slightly increased in natural cycle.

키워드

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