한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)

  • 제33권1호
  • /
  • Pages.51-55
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  • 2005
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  • 1598-642X(pISSN)
  • /
  • 2234-7305(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

배양기의 작동인자가 Thraustochytrium aureum ATCC 34304의 성장 및 Docosahexaenoic acid(DHA)의 생합성에 미치는 영향규명

Effect of Operating Factors on the Growth and DHA Biosynthesis of Thraustochytrium aureum ATCC 34304

  • 조대원 (인하대학교 공과대학교 생물공학과) ;
  • 송상규 (인하대학교 공과대학교 생물공학과) ;
  • 김원호 (인하대학교 공과대학교 생물공학과) ;
  • 허병기 (인하대학교 공과대학교 생물공학과)
  • Cho Dae-Won (Department of Biological Engineering, Inha University) ;
  • Song Sang-Kue (Department of Biological Engineering, Inha University) ;
  • Kim Won-Ho (Department of Biological Engineering, Inha University) ;
  • Hur Byung-Ki (Department of Biological Engineering, Inha University)
  • 발행 : 2005.03.01
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초록

교반속도와 배양온도가 T. aureum ATCC 3430의 성장과 지질 및 DHA 생합성에 미치는 영향을 규명하였다. 배양온도 $4^{\circ}C$내지 $39{\circ}C$범위에서 균체성장에 최적인 온도는 $32^{\circ}C$이었으며, DHA와 지질의 생성량이 가장 높은 온도는 $18^{\circ}C$이였다. 배양온도 $39^{\circ}C$ 이상과 $11^{\circ}C$이하에서는 균체의 성장량과 DHA및 지질의 생성량이 대단히 적었으나, 온도가 낮을수록 단위균체당 축적되는 지질과 DHA의 함량은 증가하여 배양온도 $4^{\circ}C$에서는 $Y_{L/X}$의 값이 $32^{\circ}C$가지, $Y_{DHA/X}$의 값은 $15\%$까지 증가하였다. 교반속도 50내지 250rpm사이에서 균체성장이 가장 높은 교반속도는 100rpm이였다. 교반속도 100과 250rpm사이에서는 교반속도가 균체의 성장량과 지질 및 DHA생합 성량에는 영향을 미치지 아니하였으나, 교반속도가 50 rpm으로 감소하면 균체 성장량과 지질 및 DHA생성량이 모두 크게 감소하였다.

The effect of rotation speed and culture temperature on the growth of T. aureum and also the biosynthesis of lipid and DHA was investigated. The optimal temperature for the growth was $32^{\circ}C$, but the best temperature for the maximum production of lipid and DHA was $18^{\circ}C$ in the range of $4^{\circ}C\;to\;39^{\circ}C$. In the case that the temperature was higher than $39^{\circ}C$ and lower than $11^{\circ}C$, the growth was very slow and the production of DHA was also very low. However, the lipid content in the biomass became higher with decrease in the culture temperature. The rotation speed for the maximum growth was 100rpm. But the growth and the production of DHA were not affected by the change of rotation speed in the range of 100 to 250 rpm. When the rotation speed was 50 rpm, the growth as well as the production of DHA and lipid was greatly reduced.

키워드

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