KOREAN JOURNAL OF CROP SCIENCE (한국작물학회지)

The Korean Society of Crop Science (한국작물학회)
권호 표지

Physiological and Biochemical Responses of Fifteen Rice Cultivars to UV-B Radiation

  • Published : 2005.03.01
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Abstract

This study was conducted to examine the physiological and biochemical responses against UV-B radiation in the seedling of 15 different rice cultivars, having the different physiological sensitivities. Out of 15 rice cultivars tested, moderate and susceptible groups showed significant decreases in biomass and RGR (relative growth rate). Contents of total chlorophyll were reduced remarkedly by irradiation of UV-B. In all rice cultivars tested, the content of chlorophyll a was strongly decreased, while the contents of chlorophyll b were slightly reduced without showing clear different among three groups and 15 cultivars. Carotenoid content was largely reduced by UV-B radiation, whereas polyamine content was moderately increased. The contents of MDA (malondialdehyde) that reflect the level of lipid peroxidation of cell membranes were clearly increased by UV-B stress, showing higher content in susceptible cultivars than moderate and torelant cultivars. The physiological important parameters highly related to visible injury were leaf color, chlorophyll, carotenoid, and lipid peroxidation, whereas biomass and polyamines were not closely correlated. Based on this results, it was concluded that changes of visible injury and the contents of chlorophyll and MDA could be adequately applied and utilized as physiological indicators to UV-B radiation.

Keywords

References

  1. Bagni, N. 1989. Polyamines in plant growth and development. In : U Bachrach, Y.M. Heimer (Esd.). The Physiology of Polyamines. vol. II, CRC Press Inc., Boca Raton. FL. pp. 107-120
  2. Barnes, P. W, S. Maggard, S R Holman, and B Vergara. 1993. Intraspecific variation in sensitivity to UV-B radiation in rice Crop Science 33: 1041-1046
  3. Besford, R. T., C M Richardson, J. L. Campos, and A F Tiburcio. 1993. Effect of polyamines on stabilization of molecular complexes of thylakoid membranes of osmotically stressed oat leaves. Planta 189 . 201-206
  4. Bouchereau, A., A. Aziz, F Larher, and J. Martin-Tanguy. 1999. Polyamines and environmental challenges recent development. Plant Sci 140. 103-125 https://doi.org/10.1016/S0168-9452(98)00218-0
  5. Caldwell, M M, A. H Teramura, M Tevini, J. F Bornman, L. O. Bjorn, and G Kulandaivelu. 1995. Effects of increased solar ultravIolet radiation on terrestrial plants. Ambio 24 : 166-173
  6. Dai, Q. J., S B. Peng, A. Q Chavez, and B. S Vergara. 1994 Intraspecific responses of 188 rice cultivars to enhanced UVB radiation. Environ. Exp Bot. 34 . 422-433
  7. D'surney, S J., T. J. Tschaplinski, N. T Edwards, and L. R. Shugart.1993. Biological responses of ten soybean cultivars exposed to enhanced UV-B radIation. Environ Exp. Bot. 33 . 347-356 https://doi.org/10.1016/0098-8472(93)90036-F
  8. FIscus, E. L., R Philbeck, A. B Britt, and F. L Booker. 1999. Growth of Arabidopsis flavonoid mutants under solar radiation and UV filters Environ. Exp Bot. 41. 231-245 https://doi.org/10.1016/S0098-8472(99)00011-8
  9. Flores, H. E and J. Martin-Tanguy. 1991. Polyamines and plant secondary metabolites, In R D. Slocum, H. E. Flores (Eds) Biochemistry and Physiology of Polyamines in Plants, CRC Press Inc., Boca Raton. FL. pp. 57-76
  10. Flores, H. E. 1991 Changes in polyamine metabolism in response to abiotic stress, In: R. D Slocum, H. E. Flores (Eds.). Biochemistry and Physiology of Polyamines in Plants. CRC Press Inc., Boca Raton. FL. pp. 214-225
  11. Galston, A. W. and R Kaur-Sawhney. 1995. Polyamines as endogenous growth regulators, In: P.J. Davies (Ed.), Plant Hormones. Physiology, Biochemistry and Molecular Biology. 2. Kluwer Academic Publishers, Boston, MA, pp. 158-178
  12. Heath, R L and L. Packer 1968. Photoperoxidation in isolated chloroplasts. I. Kinetics and stoichiometry of fatty acid peroxidation Arch. Biochem. Biophys 125. 189-198 https://doi.org/10.1016/0003-9861(68)90654-1
  13. Kumar, A, T. Altabella, M. A. Taylor, and A. F. Tiburcio. 1997. Recent advances in polyamine research Trends in Plant Sci 2. 124-130 https://doi.org/10.1016/S1360-1385(97)01013-3
  14. Li, J., T.-M. Ou- Lee, R Raba, R. G. Amundson, and R. L. Last. 1993. Arabidopsis flavonoid mutants are hypersensitive to UV-B radiation Plant Cell 5 : 171-179 https://doi.org/10.1105/tpc.5.2.171
  15. Li, Y., Y Q. Zu, J. J. Chen, H. Y. Chen, J L Yang, and Z D Hu. 2000b. Intraspecific differences in physiological responses of 20 wheat cultivars to enhanced ultraviolet-B radiation under field conditions. Environ. Exp. Bot. 44 : 95-103 https://doi.org/10.1016/S0098-8472(00)00057-5
  16. MIrecki R., M. and A. H. Teramura. 1984. Effects of ultraviolet-B irradiance on soybean. V. The dependence of plant sensitivity on the photosynthetic photon flux density during and after leaf expansion. Plant Physiol. 74. 475-480 https://doi.org/10.1104/pp.74.3.475
  17. Reggiani, R., A. Hochkoeppler, and A Bertani. 1989. Polyamines and anaerobic elongation of rice coleoptiIe Plant Cell Physiol. 30 893-898 https://doi.org/10.1093/oxfordjournals.pcp.a077821
  18. Redmond, J. W. and A Tseng. 1979 High pressure liquid chromatographic determination of putrescine, cadaverine, spermidine and spermine. J Chromatogr. 170 : 479-481 https://doi.org/10.1016/S0021-9673(00)95481-5
  19. Teramura, A H. and N S Murali. 1986 Intraspecific differences In growth and yield of soybean exposed to ultraviolet-B radiation under greenhouse and field conditions. Environ. Exp. Bot 26. 89-95 https://doi.org/10.1016/0098-8472(86)90057-2
  20. Teramura, A H. 1983. Effects of ultraviolet-B radiation on the growth and yield of crop plants. Physiol. Plant 58. 415-427 https://doi.org/10.1111/j.1399-3054.1983.tb04203.x
  21. Terarnura, A H., L. H. Ziska, and A E. Sztein. 1991. Changes in growth and photosynthetic capacity of rice with increased UVB radiation. Physiol. Plant 83 : 373-380 https://doi.org/10.1111/j.1399-3054.1991.tb00108.x