유기물자원화 (Journal of the Korea Organic Resources Recycling Association)
- 제13권3호
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- Pages.89-96
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- 2005
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- 1225-6498(pISSN)
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- 2508-3015(eISSN)
환경 독성 유기인 화합물 분해를 위하여 재조합 대장균에서 세포내 간극으로 분비된 Organophosphorus Hydrolase의 생산
Production of Periplasmic Space-Secreted Organophosphorus Hydrolase from Recombinant Escherichia coli for Degradation of Environmental Toxic Organophosphate Compounds
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Choi, Suk Soon
(Department of Environmental Engineering, Semyung University) ;
- Seo, Sang Hwan (Department of Environmental Engineering, Semyung University) ;
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Kang, Dong Gyun
(Department of Chemical Engineering, Pohang University of Science and Technology) ;
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Cha, Hyung Joon
(Department of Chemical Engineering, Pohang University of Science and Technology)
- 투고 : 2005.08.30
- 심사 : 2005.09.22
- 발행 : 2005.09.30
초록
본 연구에서는 유기인 화합물인 Paraoxon의 분해를 위하여 재조합 대장균에서 세포내 간극(periplasmic space)으로 분비되는 organophosphorus hydrolase(OPH)의 생산에 대하여 고찰하였다. OPH 생산의 향상을 위하여 성장 배지에 첨가되는 최적의 조건은 1.0 mM isopropyl-
In the present work, production of organophosphorus hydrolase (OPH) that is secreted in periplasmic space of recombinant Escherichia coli was performed for degradation of environmental toxic organophosphate compounds, paraoxon. The optimal conditions for enhancement of OPH production were 1.0 mM isopropyl-
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