Journal of Periodontal and Implant Science

Korean Academy of Periodontology (대한치주과학회)
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Role of the periosteum on bone regeneration in rabbit calvarial defects

가토의 두개골 결손부에서 골재생에 끼치는 골막의 역할

  • Jang, Hyun-Seon (Dept. of Periodontology, College of Dentistry, Chosun University, Oral Biology Research Institute, College of Dentistry, Chosun University) ;
  • Kim, Sang-Mok (Dept. of Periodontology, College of Dentistry, Chosun University) ;
  • Park, Joo-Cheol (Dept. of Oral Histology, College of Dentistry, Chosun University, Oral Biology Research Institute, College of Dentistry, Chosun University) ;
  • Kim, Byung-Ock (Dept. of Periodontology, College of Dentistry, Chosun University, Oral Biology Research Institute, College of Dentistry, Chosun University)
  • 장현선 (조선대학교 치과대학 치주과학교실, 조선대학교 치과대학 구강생물학연구소) ;
  • 김상목 (조선대학교 치과대학 치주과학교실) ;
  • 박주철 (조선대학교 치과대학 구강조직학교실, 조선대학교 치과대학 구강생물학연구소) ;
  • 김병옥 (조선대학교 치과대학 치주과학교실, 조선대학교 치과대학 구강생물학연구소)
  • Published : 2005.12.31
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Abstract

The role of the periosteum on osteointegration of $Bio-Oss^{(R)}$(Geistlich, Wolhusen/Switzerland) was studied in rabbit calvarial defect. 12 New Zealand white male rabbits between 2.8 and 4 kg were included in this randomized, blinded, prospective study. Each rabbit was anesthetized with Ketamine HCl(5 mg/kg) and Xylazine HCl(1.5 ml/kg). An incision was made to the bony cranium and the periosteum was reflected. Using a 6-mm trephine bur(3i. USA), four 8-mm defects were created with copious irrigation. The defects were classified into barrier membrane($Tefgen^{(R)}$, Lifecore Biomedical. Inc, U.S.A.) only group as a control, $Bio-Oss^{(R)}$ with barrier membrane group, $Bio-Oss^{(R)}$ with periosteum covering group, and $Bio-Oss^{(R)}$ without periosteum covering group. There were 2 rabbits in each group. The wound was closed with resorbable suture materials. Rabbits were sacrificed using phentobarbital(100 mg/kg) intravenously at 1, 2, and 4 weeks after surgery. The samples were fixed in 4% paraformaldehyde, and decalcified in hydrochloric acid decalcifying solution(Fisher Scientific, Tustin, CA) at $4^{\circ}C$ for 2-4 weeks. It was embedded in paraffin and cut into 6 ${\mu}m$ thickness. The sections were stained with H & E and observed by optical microscope. The results were as follows; 1. The periosteum played an important role in osteointegration of $Bio-Oss^{(R)}$ in bone defects. 2. When the periosteum remained intact and $Bio-Oss^{(R)}$ was placed on the defect, $Bio-Oss^{(R)}$ with periosteum covering has been incorporated into the newly formed bone from 2-week postoperatively. 3. When the periosteum was removed at the surgical procedure, invasion of connective tissue took place among the granules, and new bone formation was delayed compared to periosteum covering group. Therefore, when the bone grafting was performed with periosteal incision procedure to achieve tension-free suture, the integrity of the overlying periosteum should be maintained to avoid fibrous tissue ingrowth.

Keywords

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