Journal of Periodontal and Implant Science

Korean Academy of Periodontology (대한치주과학회)
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Evaluation of deproteinized bovine bone mineral as a bone graft substitute;A comparative analysis of basic characteristics of three commercially available bone substitutes

탈단백 우골의 골이식 대체재로서의 특성에 대한 평가;세 종류의 골 대체재의 기본 특성에 대한 비교분석

  • Park, Jin-Woo (Department of Periodontology, College of Dentistry, Kyungpook National University)
  • 박진우 (경북대학교 치과대학 치주과학교실)
  • Published : 2005.12.31
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Abstract

Bovine bone-derived bone substitutes are widely used for treatment of bone defects in dental and orthopedic regenerative surgery. The purpose of this study was to evaluate the basic characteristics of deproteinized bovine bone mineral as a bone graft substitute. Commercially available products from three different bovine bone minerals-Bio-Oss(GeistlichPharma, Switzerland), BBP(Oscotec. Korea), Osteograf/N-300(Dentsply Friadent Ceramed, USA) - were investigated. They were evaluated by scanning electron microscopy(SEM), energy dispersive X-ray spectrometer(EDS), surface area analysis(BET), and Kjeldahl protein analysis. Cell viability on different products was evaluated by 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide(MTT) assay. The results of this study indicated that each bone substitute displayed distinct surface properties. Furthermore, Kjeldahl protein analysis indicated that residual crude proteins are present in deproteinized bovine bone mineral. BBP showed relatively large amount of residual protein, which indicated that the possibility of disease transmission can not be safely ruled out. Based on the results of this study, it is suggested that active quality management is strongly needed in operations that involve processing bovine bone tissue for medical use.

Keywords

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