Journal of Periodontal and Implant Science

Korean Academy of Periodontology (대한치주과학회)
권호 표지

Effect of titanium surface roughness on adhesion and differentiation of osteoblasts

티타늄 표면조도가 조골세포의 부착 및 분화에 미치는 영향

  • Kim, Jung-Sik (Department of Periodontics, College of Dentistry, Kangnung National University) ;
  • Lee, Jae-Kwan (Department of Periodontics, College of Dentistry, Kangnung National University) ;
  • Ko, Sung-Hee (Department of Phamacology, College of Dentistry, Kangnung National University) ;
  • Um, Heung-Sik (Department of Periodontics, College of Dentistry, Kangnung National University) ;
  • Chang, Beom-Seok (Department of Periodontics, College of Dentistry, Kangnung National University)
  • 김정식 (강릉대학교 치과대학 치주과학교실) ;
  • 이재관 (강릉대학교 치과대학 치주과학교실) ;
  • 고성희 (강릉대학교 치과대학 약리학교실) ;
  • 엄흥식 (강릉대학교 치과대학 치주과학교실) ;
  • 장범석 (강릉대학교 치과대학 치주과학교실)
  • Published : 2005.12.31
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Abstract

The success of an implant is determined by its integration into the tissue surrounding the biomaterial. Surface roughness is considered to influence the behavior of adherent cells. The aim of this in vitro study was to determine the effect of surface roughness on Saos-2 osteoblast-like cells. Titanium disks blasted with 75 ${/mu}m$ aluminum oxide particles and machined titanium disks were prepared. Saos-2 were plated on the disks at a density of 50,000 cells per well in 48-well dishes. After 1 hour, 1 day, 6 days cell numbers were counted. One day, 6 days after plating, alkaline phosphatase(ALPase) activity was determined. Compared to experimental group, the number of cells was significantly higher on control group. The stimulatory effect of surface roughness on ALPase was more pronounced on the experimental group than on control group. These results demonstrate that surface roughness alters proliferation and differentiation of osteoblasts. The results also suggest that implant surface roughness may play a role in determining phenotypic expression of cells.

Keywords

References

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