Multiplication conditions in light reaction and hydrogen production in dark fermentation using Chlamydomonas reinhardtii

Chlamydomonas reinhardtii 이용한 명반응 증식 특성 및 암반응에서 수소 생산

  • Kim, Ji-Seong (Water Environment & Remediation Research Center, Korea Institute of Science and Technology) ;
  • Park, Ho-Il (Water Environment & Remediation Research Center, Korea Institute of Science and Technology) ;
  • Kim, Dong-Kun (Water Environment & Remediation Research Center, Korea Institute of Science and Technology) ;
  • Gong, Gyeng-Taek (Reaction Media Research Center, Korea Institute of Science and Technology) ;
  • Cho, Kyung-Suk (Department of Environmental Science and Engineering, Ewha Womans University) ;
  • Pak, Dae-Won (Water Environment & Remediation Research Center, Korea Institute of Science and Technology)
  • 김지성 (한국과학기술연구원 수질환경 및 복원연구센터) ;
  • 박호일 (한국과학기술연구원 수질환경 및 복원연구센터) ;
  • 김동건 (한국과학기술연구원 수질환경 및 복원연구센터) ;
  • 공경택 (한국과학기술연구원 반응매체 연구센터) ;
  • 조경숙 (이화여자대학교 과학기술대학원 환경학과) ;
  • 박대원 (한국과학기술연구원 수질환경 및 복원연구센터)
  • Published : 2005.03.15

Abstract

We experimented on growth in light and production of hydrogen and organic matters in dark fermentation by using C. reinhardtii. In the light, growth rate of C. reinhardtii following $CO_2$ fixation was proportional to consumption rate of nitrogen source. And the starch in cell was accumulated more when the period of culture was lengthened more. But the accumulation rate of starch in cell was decreased when the growth rate of cell become dull. In the dark fermentation, the production volume and production rate of hydrogen were the highest value in the mid exponential state among other states. The utilization efficiency of substrate was better in the early exponential state than other states. In production of organic matters, acetic acid didn't change remarkably and ethanol showed the highest value in early exponential state.

Keywords

References

  1. Hansel A. and Lindbald P., 'Towards optimization of cyanobacteria as biotechnologically relevant producers of olecular hydrogen, a clean and renewable energy source', Appl. Microbiol. Bio- technol., Vol. 50, 1998, pp. 153-160 https://doi.org/10.1007/s002530051270
  2. Lichtl, Rixa R., Baxin, Michael I., Hall, DAvid O., 'The biotechnology of hydrogen production by Nostoc flagelliforme grown under chemostat conditions', Applied microbiology and biotechnology, Vol. 47, 1997, pp.701-707 https://doi.org/10.1007/s002530050998
  3. Miura Y., Yamada W., Hirata K., Miyamoto K., Kiyohara M., 'Simulation of hydrogen production in algal cells grown under high $CO_2$ concentration and low temperature', Appl. Biochem. Biotechnol., Vol. 39/40, 1993, pp. 753-761 https://doi.org/10.1007/BF02919033
  4. Maeda I., Hikawa H., Miyashiro M., Yagi K., Miura Y., Miyasaka H., Akano T., Kiyohara M., Matsumoto H., Ikuta Y., 'Enhancement of starch degradation by $CO_2$ in a marine green alga, Chlamydomonas sp. MGA 161', J. Ferment. Bioeng., Vol. 78, No.5, 1994, pp. 383-385 https://doi.org/10.1016/0922-338X(94)90286-0
  5. Miura Y., 'Hydrogen production by biophotolysis based on microalgal photosynthesis', Process Biochemistry, Vol. 30, No.1, 1995, pp. 1-7
  6. Kosourov, S., Tsygankov, A, Seibert, M., Maria J., 'Sustained hydrogen photoproduction by Chlamydomonas reinhardtii: Effects of culture parameters', Biotechnology and bioengineering, Vol. 78, 2002, pp.731-740 https://doi.org/10.1002/bit.10254
  7. Elizabeth H. Harris, 'The Chlamydomonas Sourcebook : A comprehensive Guide to biology and Laboratory Use', Academic Press, Inc., 1989
  8. Kim M. S., Moon K. W., Lee I. G., Lee T. I., Sung C. K., 'Hydrogen Gas Production by Fermentation From Various Sugars Using Clostridium butyricum BCIE 9576.', Kor. J. Appl. Microbiol. Biotechnol., Vol. 27, No. 1, 1999, pp. 62-69
  9. Andrew D. Eateon, lenore S. Clesceri, Arnold E. Greenberg, ' Standatrd methods for examination of water and wastewater', APHA, 19th edit. 1995