Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Regeneration and Agrobacterium - Mediated Transient Transformation of Button Daisy (Leucanthemum vulgare)

  • Franklin G. (Crop Improvement Division, Sugarcane Breeding Institute) ;
  • Alaiwi W. Abou (Crop Improvement Division, Sugarcane Breeding Institute) ;
  • Goldman S.L. (Crop Improvement Division, Sugarcane Breeding Institute)
  • Published : 2005.03.01
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Abstract

Explants of button daisy were screened for their regeneration potential and transient GUS gene expression. Medium containing MS salts minerals and $B_5$ vitamins supplemented with $0.1\;\cal{mg/L}$ BA and $0.1\;\cal{mg/L}$ TDZ showed the best regeneration. Disc florets and receptacles were the most responsive explants in regeneration and transient gene expression respectively. Regenerated plants were successfully rooted and established in the green-house conditions. Infection and co-cultivation of explants with Agrobacterium tumefaciens containing pCAMBIA 1301 resulted in transient GUS foci. Among the different explants, receptacles showed the highest percentage of transient GUS gene expression. Enzymatic and molecular analyses of transformed calli confirmed the integration of GUS gene.

Keywords

References

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