작은땃쥐 Crocidura suaveolens와 우수리땃쥐 C. lasiura의 이하선 선세포와 분비과립의 미세구조 비교

Comparative Ultrastructure of the Acinar Cell and Secretory Granules of Parotid Salivary Gland in the Lesser White-toothed Shrew, Crocidura suaveolens and the Big White-toothed Shrew, C. lasiura

  • 정순정 (조선대학교 치과대학 구강조직학교실) ;
  • 정문진 (조선대학교 치과대학 구강조직학교실)
  • Jeong, Soon-Jeong (Department of Oral Histology, College of Dentistry, Chosun University) ;
  • Jeong, Moon-Jin (Department of Oral Histology, College of Dentistry, Chosun University)
  • 발행 : 2005.12.01

초록

이하선의 선세포와 분비과립을 작은땃쥐 Crocidura suaveolens와 우수리땃쥐 C. lasiura에서 연구하였다. 두 종 모두의 이하선은 장액선세포 한 종류만을 가지는 장액선이었고 선세포, 사이관, 과립관 그리고 줄무늬관들은 평범하였다. 작은땃쥐의 경우, 장액선세포는 잘 발달된 조면소포체, 현저한 골지체, 몇몇의 큰 미토콘드리아와 성숙 혹은 융합하고 있는 많은 중간 정도의 전자 밀도 과립들을 가지고 있었다. 미성숙 선세포의 분비과립들은 미세하고 강한 전자 밀도의 알갱이만으로 혹은 알갱이들이 주가 되었고 불분명한 경계막을 가지고 있었고, 성숙 분비과립들은 균일하고 옅은 중심부를 가지고 그 주변에 미세하고 강한 전자밀도의 알갱이들과 명확한 경계막을 가지고 있었다. 우수리땃쥐의 경우, 선세포는 잘 발달된 조면소포체, 현저한 골지체, 몇몇의 큰 미토콘드리아 뿐 아니라 성숙 혹은 융합하고 있는 많은 진한 전자 밀도의 분비과립들을 가지고 있었다. 미성숙 선세포 분비과립들은 옅고 거친 알갱이들만으로 채워져 있었으며 불명확한 경계막을 가지고 있었고, 성숙 분비과립들은 진하고 거친 알갱이들만으로 채워져 있었고 명확한 경계막을 가지고 있었다. 결국, 작은땃쥐의 이하선 분비과립은 광학과 전자현미경 수준에서 중간 정도의 전자밀도로, 우수리땃쥐의 분비과립은 광학 현미경 수준에서는 아주 진하게, 전자현미경에서는 진하게 관찰되었다.

The acinar cells and secretory granules of the parotid salivary gland were examined in the lesser white toothed shrew, Crocidura suaveolens and the big white-toothed shrew, C. lasiura. The parotid gland of both species were a serous gland having only one kind of serous acinar cells, and had conventional arrangement of acini and intercalated, granular and striated ducts. In case of C. suaveolens, serous acinar cells had well developed rER, prominent Golgi complex, several large mitochondria and abundant moderate dense secretory granules with various stages of the maturing or fusing process. Immature acinar secretory granules were only or mainly filled with fine strong dense specks and had an indistinct limiting membrane, and mature granules were filled with homogeneous pale large round center and had fine strong dense specks at the periphery of the homogeneous pale center and a distinct limiting membrane. In case of C. lasiula, serous acinar cells had well developed rER, prominent Golgi complex, several large mitochondria and abundant dense secretory granules with maturing or fusing process. Immature acinar secretory granules were only filled with pale rough specks and had an indistinct limiting membrane, and mature granules were only filled with rough dense specks and had a distinct limiting membrane. Eventually The acinar secretory granules of C. suaveolens were seen moderate at the light and ultrastuctural level, those of C. lasiura were strong dense at the light microscopic level and dense at the ultrastructural level.

키워드

참고문헌

  1. Ball WD: Cell restricted secretory proteins as markers of cellular phenotype in salivary glands. In Dobrosielski-Vergona K (ed) Biology of the salivary glands. CRC Press Boca Raton pp.355-396, 1993
  2. Carson KA, Rose RK: Fine structure of the submandibular salivary gland of the Venomous short-tailed shrew, Blarina brevicauda (Insectivora: Soricidae). European J Morph 31 : 111-128, 1993
  3. Churchfield S: The natural history of Shrews. A&C black Ltd London, 1990
  4. Eisenberg JF: The mammalian radiations-an analysis of trends in evolution, adaptation and behavior. University of Chicago Press Chicago IL 1981
  5. Han SH: Phylogenetic study of the small mammals in Korea. Ph D dissertation University of Hokkaido 1997
  6. Hand AR: Salivary glands. In Bhaskar SN (ed) Orban's oral histology and embryology. Mosby St Louis pp. 336-370, 1980
  7. Jacobsen N, Hensten Petersen A: Some biochemical characteristicsof the salivary gland products of Macaca irus and Cercopitheus aethiops. Comp Biochem Physiol 49 : 605-641, 1974 https://doi.org/10.1016/0305-0491(74)90246-6
  8. Jeong SJ, Yoon MH: Spermatogenesis in three Korean shrews and noptes on their phylogenetic significanc. Korean Journal of Life Science 11 : 218-229, 2001
  9. Jeong SJ, Lim DS, Park JC, Kim HJ, Jeong JO, Choi BD, Yoon MH, Jeong MJ: Ultrastructure of the submandibular gland in the big white-toothed shrew, Crocidura lasiura. Korean J Electron Microscopy 35 : 57-64, 2005a
  10. Jeong SJ, Jeong MJ, Kim DK, Kook JK, Kim HJ, Yoon MH, Park JC: Ultrastructure of the submandibular gland in the lesser white-toothed shrew, Crocidura suaveolens. Korean J Electron Microscopy 35 : 65-72, 2005b
  11. Jones JK, Johnson DH: Review of the Insectivores of Korea. Univ Kansas Publ Mus Nat Hist 9 : 551-578, 1960
  12. Levine MJ, Reddy MS, Tabak LA, Loomis RE, Bergey EJ, Jones PC, Choen RE, Stinson MW, Al Hashimi I: Structural aspect of salivary glycoproteins. J Dental Res 66 : 436-441, 1987 https://doi.org/10.1177/00220345870660020901
  13. Mineda T: Histological and histochemical investigations on the salivary glands of the black and refous elephant shrew (Rhynchocyon chrysopygus). J Dent Aichi-Gakuin univ 19 : 78-85, 1981
  14. Mineda T: Fine structure of salivary gland and cytomegalovirus of the House musk shrew, Suncus murinus. In Oda S, Kitoh J, Ota K and Isomura G (eds.) Suncus murinus-Biology of the laboratory shrew. Japan scientific societies press (JSSP) pp.370-388, 1985
  15. Nagato T, Tandler B, Phillips CJ: Unusual smooth endoplasmic reticulum in submandibular acinar cells of the male round-eared bat, Tonatia sylvicola. J ultrastruct Res 87 : 275-284, 1984 https://doi.org/10.1016/S0022-5320(84)80066-0
  16. Phillips CJ, Tandler B: Mammalian evolution at the cellular level. Current mammalogy 1 : 1-66, 1987
  17. Phillips CJ, Nagato T, Tandler B: Comparative ultrastructure and evolutionary patterns of acinar secretory product of parotid salivary glands in Neotropical bats. Zoology 39 : 213-229, 1987a
  18. Phillips CJ, Tandler B, Pinkstaff CA: Uniques salivary glands in two genera of tropical microchiropteran bats-An example of evolutionary convergence in histology and histochemistry. J Mammal 68 : 235-242, 1987b https://doi.org/10.2307/1381462
  19. Phillips CJ, Tandler B, Nagato T: evolutionary diversity of salivary gland acinar cells-a format for understanding molecular evolution. In Dobrosielski-Vergona K (ed) The biology of the salivary glands. CRC Press Boca Raton pp.41-80, 1993
  20. Robinorich MR, Sreebny LM: Separation and identification of some of the protein components of rat parotid saliva. Archives of oral biology 14 : 935-949, 1969 https://doi.org/10.1016/0003-9969(69)90270-2
  21. Rothman JE, Orci L: Molecular dissection of the secretory pathway. Nature 355 : 409-415, 1992 https://doi.org/10.1038/355409a0
  22. Tandler B: Microstructure of salivary glands. In Rowe NH (ed) A symposium on salivary glands and their secretion. Uinversity of Michigan Press Ann Arbor pp.8-21, 1972
  23. Tandler B, Phillips CJ: Structure of serous cells in salivary glands. Microscopy Res Tech 26 : 32-48, 1993 https://doi.org/10.1002/jemt.1070260105
  24. Tandler B, Phillips CJ, NAgato T: Parotid salivary gland ultrastructure in an omnivorous neotropical bat: evolutionary diversity at the cellular level. Zoologica Scripta 4 : 419-427, 1988
  25. Tandler B, Phillips CJ, Nagato T, Toyoshima K: Ultrastructural diversity in chiropteran salivary glands. In Riva A and Motta PM (eds) Ultrastructure of the Extrapariental glands of the Digestive tract. Kluwer academic publishers pp.31-52, 1990
  26. Tandler B, Phillips CH, Pinkstaff CA: Mucous droplets with multiple membranes in the accessory submandibular glands of Long-winged bats. Anat Rec 240 : 178-188, 1994 https://doi.org/10.1002/ar.1092400205
  27. Wallach D, Tessler R, Schramm, M: The proteins of the content of the secretory granules of the rat parotid gland. Biochemica et Biophysica Acta 382 : 552-564, 1975 https://doi.org/10.1016/0005-2736(75)90222-9
  28. Won PH: Illustrated encyclopedia and fauna and flora of Korea. Vol 7. Korea Ministry of education pp. 259-283, 1967
  29. Yoon MH, Jeong SJ: Ultrastructure of epididymal spermatozoa in three Korean shrews. Korean Journal of Life Science 11 : 54-61, 2001