Korean Journal of Ecology and Environment (생태와환경)

The Korean Society of Limnology (한국수생태학회)
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Effects of Nonylphenol and Diethylhexyl Phthalate on the Population Growth of Freshwater HABs, Microcystis and Stephanodiscus

내분비교란물질이 조류의 성장에 미치는 영향

  • Lee, Ju-Han (Department of Life Science, Hanyang University) ;
  • Kim, Baik-Ho (Department of Life Science, Hanyang University) ;
  • Han, Myung-Soo (Department of Life Science, Hanyang University)
  • Published : 2005.09.30
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Abstract

Effects of two endocrine disrupters (EDs) , nonylphenol (NP) and diethylhexyl phthalate (DEHP), on the population growth and morphology of two freshwater HABs (harmful algal blooms) , Microcystis aeruginosa and Stephanodiscus hantzschii, which frequently evoked the hazard bloom in an eutrophic lakes and reservoirs worldwide, were examined with seven different concentrations of EDs (0.01, 0.05, 0.1, 1,2, 2.5 and 3 ppm). Even at concentration below 0.01 ppm, NP strongly inhibited the algal growth of both M. aeruginosa and S. hantzschii, regardless of the algal growth phase. Morphologically, the algal cell treated with NP gradually lost green color in cytoplasm, became smaller in cell size, and then, was hardly seen in microscopic field. On the other hand, DEHP employed did not affect two algae at all concentrations, and rather stimulated the growth by about 10% with a treatment at 3.00 ppm compared to control. These results indicate that the continuous input of EDs, DEHP into the natural water system plays a crucial role to enhance or help an outbreak of algal bloom in eutrophic waters.

내분비계 장애물질이 식물플랑크톤의 개체군 성장에 미치는 영향을 이해하고자 부영양 호수 및 하천에서 우점하는 남조 M. aeruginosa와 규조 S. hantzschii를 성장시기에 따라 자연수 농도의 10 ${\sim}$ l,000배까지 다양한 농도로 투여하고 현존량 및 형태적 변화를 조사하였다. Nonylphenol (NP)을 조류세포 배양초기나 대수성장기에 처리하였을 경우 농도 0.01 ppm이상에서 곧바로 성장억제 현상을 보였으며, 남조 M. aeruginosa가 규조 S. hantzschii보다 더 심한 억제현상을 보였다. 이에 반해 diethyhexyl phthalate(DEHP)의 경우에는 조류세포 성장시기나 처리농도에 상관없이 성장 억제 현상은 관찰되지 않았고, 실험 중 가장 높은 처리농도인 3.00 ppm 처리군에서는 오히려 대조군보다 10% 이상의 높은 성장을 나타냈다. 조류세포의 형태적 변화는 NP를 처리한 모든 실험군에서 조류세포의 색소체 변화가 뚜렷하였으며, M. aeruginosa 세포의 경우, 크기가 점차 작아지는 특징을 보였다. 결국 DEHP의 자연계로의 유출은 식물플랑크톤을 섭식하는 섬모충이나 동물플랑크톤의 성장을 억제하거나 M. aeruginosa 및 S. hantzschii와 같은 유해조류의 성장을 촉진함으로써 부영양수역 조류대발생의 중요한 요인으로 작용될 수 있음을 시사해주고 있다.

Keywords

References

  1. 곽인실, 이원철. 2005. 내분비교란 물질에 노출된 C. plumosus (장수깔다구)의 하순기절기형성. 한국육수학회지 38: 11- 17
  2. 김만영. 1998. 플라스틱과 내분비교란물질: 내분비교란물질 관 련 국내외 동향과 소비자 안전 확보 대책. 강원대 부속 환 경연구소 학술심포지엄. 21-40
  3. 김성철, 박청길, 조현서, 이대인. 2003. 낙동강 퇴적물의 Nonylphenol과 Bisphenol A 오염도 평가. 한국물환경학회지 19: 357-366
  4. 김종훈. 2002a. 물 시료 중 Octylphenol, Nonylphenol, Di (2- ethylhexyl)phthalate의 연구. Analytical Science & Technology 15: 172-179
  5. 김종훈. 2002b. 하수슬러지 중 Nonylphenol, Octylphenol, Di (2-ethylhexyl)phthalate의 연구. Analytical Science & Technology 15: 451-458
  6. Ahel, M., W. Giger and C. Schaffner. 1994. Behaviour of alkylphenol polyethoxylate surfactants in the aquatic environment-II. Occurrence and transformation in rivers. Water Res. 28: 1143-1152 https://doi.org/10.1016/0043-1354(94)90201-1
  7. Amaral Mendes, J.J. 2002. The endocrine disrupters: a major medical challenge. Food Chem. Toxicol. 40: 781- 788 https://doi.org/10.1016/S0278-6915(02)00018-2
  8. Beaker, G., H.M. Canter and G.H.M. Jaworski. 1988. Zoospores ultrastructure of Zygorhizidium affluens Canter and Z. planktonicum Canter, two chytrids parasitizing the diatom Asterionella formosa Hassall. Can. J. Bot. 66: 1054-1067 https://doi.org/10.1139/b88-151
  9. Bettinetti, R., D. Cuccato, S. Galassi and A. Provini. 2002. Toxicity of 4-nonylphenol in spiked sediment to three populations of Chironomus riparius. Chemosphere. 46: 201-207 https://doi.org/10.1016/S0045-6535(01)00114-X
  10. Blackburn, M.A. and M.J. Waldock. 1995. Concentrations of alkylphenols in rivers and estuaries in England and Wales. Water Res. 29: 1623-1629 https://doi.org/10.1016/0043-1354(94)00340-D
  11. Dunier, M. and A.K. Siwicki. 1993. Effects of pesticides and other organic pollutants in the aquatic environment on immunity of fish: a review. Fish Shellfish Immunol. 3: 423-438 https://doi.org/10.1006/fsim.1993.1042
  12. EPA. 1998. Research Plan for Endocrine Disruptors
  13. Eriksson, C. and H. Burton. 2003. Origins and biological accumulation of small plastic particles in fur seals from Macquarie Island. AMBIO 32: 380-384
  14. Ferrara, F., F. Fabietti, M. Delise, A.P. Bocca and E. Funari. 2001. Alkylphenolic compounds in edible mollusks of the Adriatic sea (Italy). Environ. Sci. Technol. 35: 3109-3112 https://doi.org/10.1021/es010508h
  15. Fromme, H., T.K. Kuchler, T. Otto, K. Pilz, J. Muller and A. Wenzel. 2002. Occurrence of phthalates and bisphenol A and F in the environment. Water Res. 36: 1429-1438 https://doi.org/10.1016/S0043-1354(01)00367-0
  16. Giger, W., P.H. Brunne and C. Schaffner. 1984. 4-Nonylphenol in sewage sludge: accumulation of toxic metabolites from nonionic surfactants. Science 225: 623-625 https://doi.org/10.1126/science.6740328
  17. Gimeno, S., H. Komen, A. Gerritsen and T. Bowmer. 1998. Feminization of young males of the common carp, Cyprinus carpio, exposed to 4-tert-pentylphenol during sexual differentiation. Aquatic Toxicol. 43: 77-92 https://doi.org/10.1016/S0166-445X(98)00056-3
  18. Gray, M.A. and C.D. Metcalfe. 1997. Induction of testisova in Japanese medaka (Oryzias latipes) exposed to pnonylphenol. Environ. Toxicol. Chem. 16: 203-218
  19. Guruge K.S. and S. Tanabe. 2001. Contamination by persistent organochlorines and butyltin compounds in the West Coast of Sri Lanka. Mar. Pollut. Bull. 42: 179-186 https://doi.org/10.1016/S0025-326X(00)00140-5
  20. Ha, K., M.H. Jang and G.J. Joo. 2002. Spatial and temporal dynamics of phytoplankton communities along a regulated river system, the Nakdong River, Korea. Hydrobiologia 470: 235-245 https://doi.org/10.1023/A:1015610900467
  21. Han, M.-S., Y. YAug, J.K. Ryu, K.I. Yoo and Y.K. Choi. 1995. Ecological studies on Pal'tang River-Reservoir system in Korea 2. Changes in phytoplankton community structure. Kor. J. Limnol. 28: 335-344
  22. Harino, H., M. Fukushima and S. Kawai. 2000. Accumulation of butylin and phenyltin compounds in various fish species. Arch. Environ. Contam. Toxicol. 39: 13-19 https://doi.org/10.1007/s002440010074
  23. Kashiwada, S., H. Ishikawa, N. Miyamoto, Y. Ohnishi and Y. Magara. 2002. Fish test for endocrine-disruption and estimation of water quality of Japanese rivers. Water Res. 36: 2161-2166 https://doi.org/10.1016/S0043-1354(01)00406-7
  24. Kwak, I.S. and W.C. Lee. 2004. Detecting point for ecological disruptions and developmental delay exposure to DEHP in Chironomus riparius (Diptera: Chironomidae). Kor. J. Environ. Biol. 22: 321-328
  25. Li, D.H., M.S. Kim, W.J. Shin, U.H. Yim, J.R. Oh and Y.J. Kwon. 2004. Seasonal flux of nonylphenol in Han River, Korea. Chemosphere. 56: 1-6 https://doi.org/10.1016/j.chemosphere.2004.01.034
  26. Mann, R.M. and J.R. Bidwell. 2000. Application of the FETAX protocol to assess the developmental toxicity of nonylphenol ethoxylate to Xenopus laevis and two Australian frogs. Aquatic Toxicol. 51: 19-29 https://doi.org/10.1016/S0166-445X(00)00106-5
  27. Manning, C.S., T.F. Lytle, W.W. Walker and J.S. Lytle. 1999. Life-cycle toxicity of bis (tributyltin) oxide to the sheepshead Minnow (Cyprinodon variegatus). Arch. Environ. Contam. Toxicol. 37: 258-266 https://doi.org/10.1007/s002449900513
  28. Matozzo, V., M. Deppieri, V. Moschino and M.G. Marin. 2003. Evaluation of 4-nonylphenol toxicity in the clam Tapes philippinarum. Environ. Res. 91: 179-185 https://doi.org/10.1016/S0013-9351(02)00052-X
  29. Meregalli, G., L. Pluymers and F. Ollevier. 2001. Induction of mouthpart deformities in Chironomus riparius larvae exposed to 4-n-nonylphenol. Environ. Pollution. 111: 241-246 https://doi.org/10.1016/S0269-7491(00)00068-3
  30. Nakamura Y., Y. Yagi, I. Tomita and K. Tsuchikawa. 1979. Teratogenicity of di- (2-ethylhexyl)phthalate in mice. Toxicol. letters. 4: 113-117 https://doi.org/10.1016/0378-4274(79)90084-5
  31. Naylor, C.G. 1992. Alkylphenol ethoxylates in the environment. J. Offic. Anal. Chem. Soc. 69: 695-703
  32. OECD. 1996. 24th Joint Meeting of the Chemicals Group and Management Committee (II)
  33. Schwaiger, J., O.H. Spieser, C. Bauer, H. Ferling, U. Mallow, W. Kalbfus and R.D. Negele. 2000. Chronic toxicity of nonylphenol and ethinylestradiol: haematological and histopathological effects in juvenile Common carp (Cyprinus carpio). Aquatic Toxicol. 51: 69-78 https://doi.org/10.1016/S0166-445X(00)00098-9
  34. Sibley, P.K., K.R. Solomon, S. Mabury and J. Sall. 2000. Microcosm assessment of the environmental fate and biological effect of nonylphenol. Guelph Turfgrass Institut. Annual Res. Report
  35. SIDS. 1995. for High Production Volume Chemicals
  36. Soto, A.M., H. Justicia, J.W. Wray and C. Sonnenschein. 1991. P-nonylphenol: an estrogenic xenobiotic released from modified polystyrene. Environ. Health Perspect 102: 380-383 https://doi.org/10.2307/3431626
  37. SPSS Inc. 2003. SPSS Base 12.0 for window, SPSS Inc., 444N. Michigan Avenue, Chicago, IL., 60611
  38. Sugiura, K. 1996. The use of an aquatic microcosm for pollution effects assessment. Water Res. 30: 1801-1812 https://doi.org/10.1016/0043-1354(96)00062-0
  39. Tanaka, Y. and J. Nakanishi. 2002. Chronic effects of pnonylphenol on survival and reproduction of Daphnia galeata: multigenerational life table experiment. Institute of Environ. Sci. Technol. 29: 487-492
  40. Traunspurger, W., H. Schafer and A. Femde. 1996. Comparative investigation of the effect of herbicide on aquatic organism in single species tests and aquatic microcosms. Chemosphere. 33: 1129-1141 https://doi.org/10.1016/0045-6535(96)00252-4
  41. Tsuda, T., A. Takino, K. Muraki, H. Harada and M. Kojima. 2001. Evaluation of 4-nonylphenols and 4-tert-octylphenol contamination of fish in rivers by laboratory accumulation and excretion experiments. Water Res. 35: 1786-1792 https://doi.org/10.1016/S0043-1354(00)00445-0
  42. Watanabe, M.M. and M. Hiroki. 1997. NIES-Colletion: list of strains, Microalgae and Protozoa, 5th edn. Microbial Culture Collection Institute for Environmental Studies, Tsukuba. p. 140
  43. Yadetie, F. and R. Male. 2002. Effects of 4-nonylphenol on gene expression of pituitary hormones in juvenile Atlantic salmon (Salmo salar). Aquatic Toxicol. 58: 113- 129 https://doi.org/10.1016/S0166-445X(01)00242-9
  44. Yasuno, M., Y. Sugaya, K. Kaya and M.M. Watanabe. 1998. Variations in the toxicity of Microcystis species to Moina macrocopa. Phycol. Res. 46: 31-36 https://doi.org/10.1111/j.1440-1835.1998.tb00268.x