Journal of Plant Biotechnology

한국식물생명공학회 (The Korean Society of Plant Biotechnology)
권호 표지

De novo Regeneration of Fertile Common Bean (Phaseolus vulgaris L.) Plants

  • Albino Margareth M.C. (Embrapa Recursos Geneticos e Biotecnologia, Laboratorio de Introducao e Expressao de Genes, Universidade de Brasilia, Departamento de Botanica, Instituto de Ciencias Biologicas) ;
  • Vianna Giovanni R. (Embrapa Recursos Geneticos e Biotecnologia, Laboratorio de Introducao e Expressao de Genes) ;
  • Falcao Rosana (Embrapa Recursos Geneticos e Biotecnologia, Laboratorio de Introducao e Expressao de Genes) ;
  • Aragao Francisco J.L. (Embrapa Recursos Geneticos e Biotecnologia, Laboratorio de Introducao e Expressao de Genes)
  • 발행 : 2005.12.01
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초록

Common bean (Phaseolus vulgaris L.) plants were regenerated via organogenesis from mature embryonic axes, cultured on MS medium supplemented with ildole-3-ecetic acid (IAA) and thidiazuron (TDZ) for one week in the dark. Embryonic axillary regions were excised, longitudinally cut to split the both sides, and cultured for two weeks on MS medium supplemented with IAA and TDZ. The combination 0.5 mg $l^{-1}$ TDZ/0.5 mg $l^{-1}$ IAA presented the higher efficiency in shoot regeneration and the combination 0.5 mg $l^{-1}$ TDZ/0.25 mg $l^{-1}$ IAA presented the higher efficiency in conversion of shoots to plants. Regenerating explants were transferred to MS medium containing 1 mg $l^{-1}$ BAP for shoot development. All elongated shoots were rooted in vitro, presented normal phenotype and produced viable seeds. Histological analysis confirmed the mode of regeneration as de novo shoot organogenesis.

키워드

참고문헌

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