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산호수 (Ardisia pusilla DC.)의 기내 대량번식

In vitro Mass Propagation of Ardisia pusilla DC.

  • 강관호 (오윤바이오텍, 전북대학교 농업생명과학대학) ;
  • 오월선 (오윤바이오텍, 전북대학교 농업생명과학대학) ;
  • 구대회 (원예연구소 화훼과) ;
  • 은종선 (전북대학교 농업생명과학대학) ;
  • 김형무 (전북대학교 농업생명과학대학)
  • Kang Gwan-Ho (Ohyoonbiotech Co. College of Agriculture & Life Sciences, Chonbuk National University) ;
  • Oh Owel-Sun (Ohyoonbiotech Co. College of Agriculture & Life Sciences, Chonbuk National University) ;
  • Goo Dae-Hoe (National Horticultural Research Institute, RDA) ;
  • Eun Jong-Seon (College of Agriculture & Life Sciences, Chonbuk National University) ;
  • Kim Hyung-Moo (College of Agriculture & Life Sciences, Chonbuk National University)
  • 발행 : 2005.12.01

초록

우리나라 자생식물인 산호수 (Ardisia pusilla DC)를 기내에서 대량증식하기 위하여 초대배양으로 산호수 경정을 MS, 1/2MS 배지에 BA와 TDZ의 농도를 달리하여 첨가된 배지에 치상하여 배양하였다. 무기염류를 달리한 배지와 생장조절물질 농도를 다르게 한 배지 중 1/2MS 배지에 BA 0.5mL가 첨가된 배지가 신초 수 7개 이상으로 증식이 양호하여 산호수의 초대배양에 적정배지로 나타났다. TDZ를 첨가한 배지에서는 캘러스형성이 과다하여 부정아 형성을 오히려 방해하여 BA 첨가배지보다 신초증식이 양호하지 못하였다. 신초의 증식을 위해 1/2 MS 배지에 BA 0.5 mg와 sucrose의 농도를 달리하여 첨가한 결과, sucrose 농도 20 g/L를 첨가한 배지에서 신초의 증식, 생장 및 다아체 형성이 양호하였다. 형성된 신초의 발근은 1/2MS 배지에 IBA, NAA를 각각 첨가한 배지를 사용하였는데 두 가지 옥신류에서 0.5 mg의 농도에서 신초의 생장과 발근이 양호하였다. 잎과 뿌리가 잘 발달한 유식물체를 원예용 상토에 옮겨 키웠을 때 모든 개체가 정상적인 형태로 성장하였다.

To establish the mass proliferation system of Ardisia pusilla DC, the shoot tips of Ardisia pusilla DC were cultured on the MS and half-strength MS medium supplemented with $0{\sim}5.0$ mg/L BA or $0{\sim}0.5$ mg/L thidiazuron(TDZ), respectively. A few multiple shoot formation observed when the shoots were cultured on MS medium containing TDZ. However, the frequency of multiple shoot formation was reached up to 82.4%, when the shoots were cultured on half-strength MS medium supplemented with 0.5 mg/L BA. Also the number of shoot per explant was 7.1. To promote rooting from multiple shoot, newly formed shoots were transferred to half-strength MS medium containing 0.5 mg/L IBA or 0.5 mg/L NAA, respectively. Regenerated plantlets were grown to normal mature plants in soil.

키워드

참고문헌

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