Journal of Plant Biotechnology

  • 제32권2호
  • /
  • Pages.139-144
  • /
  • 2005
  • /
  • 1229-2818(pISSN)
  • /
  • 2384-1397(eISSN)
한국식물생명공학회 (The Korean Society of Plant Biotechnology)
권호 표지
DOI QR코드

DOI QR Code

생장억제제 처리가 기내 배양한 나도풍란 (Sedirea japonica) 유묘의 ABA 유사물질 함량 및 GA 유사물질 활성에 미치는 영향

Effect of Growth Retardants on Endogenous ABA-like Substance Content and GA-like Substance Activity of Sedirea japonica Seedlings Cultured In Vitro

  • Cho Dong-Hoon (Department of Horticulture, Kyungpook National University) ;
  • Jee Sun-Ok (Department of Biotechnology, Joongbu University)
  • 발행 : 2005.06.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

본 연구는 기내 배양한 나도 풍란에 식물생장억제제인 Uniconazole, Ancymidol, Paclobutrazol의 종류별, 농도별 처리가 식물체내 내생 GA 유사물질의 활성 및 ABA 유사물질의 함량의 변화에 미치는 영향을 알아보기 위하여 실시되었다. 지상부인 잎에서는 Uniconazole 0.05 mg/L, Ancymidol 0.1 mg/L, Paclobutrazol 0.3 mg/L 처리 시 ABA 유사물질의 함량은 대조구보다 낮았으며, GA유사물질의 활성은 대조구 보다 높거나 비슷한 수준이었다. 하지만 처리농도가 높아짐에 따라 ABA 유사물질의 농도는 높아졌으나, GA 유사물질의 활성은 비교적 억제되었다. 지하부인 뿌리에서는 Uniconazole 0.05, 0.2mg/L, Ancymidol 0.2 mg/L, Paclobutrazol 0.1 mg/L 처리에서 ABA 유사물질의 함량이 대조구보다 낮았고, 그 이상의 농도에서는 높았다. GA 유사물질의 활성은 비교적 저 농도에서는 높았으나, 고농도 처리에서는 저 농도와 비교하여 활성이 억제되었다.

This experiment was conducted to identify the effect of several plant growth retardants on endogenous ABA-like substance content and GA-like substance activity in seedlings of Sedirea japonica cultured in vitro. When seedlings of Sedirea japonica were treated with low concentration of 0.05 mg/L Uniconazole, 0.1 mg/L Ancymidol and 0.3 mg/L Paclobutrazol, the content of ABA-like substances of the leaf was lower than that of the control. However, the activity of GA-like substances was similar or higher in treated seedlings. In the mid and high concentrations of three kinds of growth retardants, the ABA-like substance content was increased, but GA-like substance activity was inhibited. The content of ABA-like substances in the root was lower in 0.05 and 0.2 mg/L Uniconazole, 0.2 mg/L Ancymidol and 0.1 mg/L Paclobutrazol treatments than that of the control, but in the mid and high concentration treatments, the content was increased. GA-like substance activity in low concentration was increased but in the mid and high concentration, the activity was inhibited compared with low concentration treatment.

키워드

참고문헌

  1. An JK, Choi YW, Son BG, Cho D (1997) Introduction of plant hormones. Miryang national university press, pp 141-169
  2. Badr SA, Martin GC, Hartmann HT (1971) A modified method for extraction and identification of abscisic acid and gibberellin-like substances from the olive (Olea europaea). Physiol Plant 24: 191-198 https://doi.org/10.1111/j.1399-3054.1971.tb03477.x
  3. Barret JE (1982) Chrysanthemum height control by ancymidol, PP333, and EL-500 dependent on medium composition. HortSci 17: 896-897
  4. Cho DH, Chung MY, Kim CK, Kim HY, Jee SO, Shin SR, Chung JD (2004) Effect of growth retardants on the growth of Sedirea japonica. Kor J Hort Sci Technol 22: 95-99
  5. Choi JM, Choi JJ, Chung HJ, Choi JS (1998) Growth of Oriental hybrid lily 'Star Gazer' affected by application method and concentration of uniconazole in pot plant production. J Kor Soc Hort Sci 39: 776-779
  6. Chung JD, Chun CK, Kim SS (1983) Asymbiotic Germination and Plant Culture of Aerides japonicum (I) Selection of Suitable Medium and Culture Condition for Germination and Effect of Hyponex, Peptone, and Several Nutrient Supplements on Growth of Seedlings. HORTICULTURE ABSTRACTS Vol. 1 No.2, pp. 52-53
  7. Chung JD, Park YK, Kim HY, Jee SO, Koh JC (1999) Effects of Plant Growth Retardants on the Growth of Bletilla striata in vitro. J Kor Soc Hort Sci 40: 485-488
  8. Coolbaugh Re., Hirano S, West C (1965) Studies on the specialtyand site of action of ${\alpha}$-cyclopropyl-${\alpha}$-(p-methoxyphenyl)-5-pyrimidine methyl alcohol (ancymidol), a plant growth regulator. Plant Physiol 62: 571-576 https://doi.org/10.1104/pp.62.4.571
  9. Kim HY, Suzuki Y (1989) Changes in assimilated $^{13}C$ istribution and soluble acid invertase activity of Zinnia legance induced by uniconazole an inhibitor of gibberellin iosynthesis. Plant Physiol 90: 316-321 https://doi.org/10.1104/pp.90.1.316
  10. Konoshima H, Tsukamoto Y (1978) The role of leaves in the induction of dormancy of gladiolus corm. J Jap Soc Hort Sci 46: 509-514 https://doi.org/10.2503/jjshs.46.509
  11. Larson RA (1985) Growth regulators in floriculture. Hort Rev 7: 399-481
  12. Lee CB (1989) Illustrated flora of Korea. Hyangmunsa Seoul, pp 246
  13. Lee J (1985) The wild orchid of korea. Jangsung publisher, Seoul, pp. 201-204
  14. Million JB, Barrett JF, Nell TA, Clark DG (1998) Influence of media components on efficiency of paclobutrazol in inhibiting growth of broccoli and petunia. HortSci 33: 852-856
  15. Nagao MA, Ho-a EB, Yoshimoto JM (1999) Unlconezole Retardants Growth and Increases Flowering of Young Macademia Trees. HortSci 34: 104-105
  16. Newman SE, Tant JS (1995) Root-zone medium influences growth of poinsettias treated with paclobutrazol-impregnated spikes and drenches. HortSci 30: 1403-1405
  17. Park JS (2000) Studies on habitat environment and flowering physiology of Cymbidium goeringii. PhD thesis, Kyungpook national university, Daegu
  18. Park JS, Chung JD, Kim HY (2001) Influence of Uniconazole and GA on Flower bud formation, carbohydrate content and GA-Iike substance activity of Cymbidium goeringii. J Kor Soc Hort Sci 42: 591-595
  19. Park YK (2000) Effect of plant growth retardants on the growth of Aerides japonicum and Bletilla striata cultured in vitro. MS thesis, Kyungpook national university, Daegu
  20. Park IH, Suh YK, Chun CK (1988) Studies on the Dormancy of the Corm of Liatris spicata Willd. ; II. Seasonal Variation of Activity of Endogenous Gibberellin-like Substances. HORTICULTURE ABSTRACTS Vol. 3 No. 2 pp. 108-109
  21. Sembdner G, Weiland J, Schneider G, Schreiber K, Focke I (1970) Recent advances in the metabolism of gibberellins. In : Carr DJ (eds), Plant Growth substances. Springer-Verlag Berlin, pp 143-150