Journal of Plant Biotechnology

  • 제32권2호
  • /
  • Pages.97-103
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  • 2005
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  • 1229-2818(pISSN)
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  • 2384-1397(eISSN)
한국식물생명공학회 (The Korean Society of Plant Biotechnology)
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Superoxide Dismutase와 Ascorbate Peroxidase가 엽록체내로 동시에 과대발현된 형질전환 담배의 제초제들에 대한 항산화 반응

Antioxidative Responses of Transgenic Tobacco Plants Expressing both Superoxide Dismutase and Ascorbate Peroxidase in Chloroplasts to Several Herbicides

  • 김진석 (한국화학연구원 생물기능연구팀) ;
  • 이병회 (한국화학연구원 생물기능연구팀) ;
  • 권석윤 (한국생명공학연구원 환경생명공학연구실) ;
  • 김윤희 (한국생명공학연구원 환경생명공학연구실) ;
  • 김소희 (한국화학연구원 생물기능연구팀) ;
  • 조광연 (한국화학연구원 생물기능연구팀)
  • Kim Jin-Seog (Biological Function Research Team, Korea Research Institute of Chemical Technology (KRICT)) ;
  • Lee Byung-Hoi (Biological Function Research Team, Korea Research Institute of Chemical Technology (KRICT)) ;
  • Kwon Suk-Yoon (Labaratory of Environmental Biotechnology, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Kim Yun-Hee (Labaratory of Environmental Biotechnology, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Kim So-Hee (Biological Function Research Team, Korea Research Institute of Chemical Technology (KRICT)) ;
  • Cho Kwang-Yun (Biological Function Research Team, Korea Research Institute of Chemical Technology (KRICT))
  • 발행 : 2005.06.01
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초록

Superoxide dismutase와 ascorbate peroxidase가 동시에 과대발현된 담배(CA)를 가지고 여러 제초제들에 대한 항산화 반응을 조사하였다. 온실조건 실험에서, CA 담배는 PSI 저해제로 알려진 paraquat처리에 대해서 내성이 인정되었고 그 정도는 40% 내외였다. PS II 저해제 (bromoxynil, diuron, bromacil), 엽록소 생합성 저해제 (oxyfluorfen), 카로티노이드 생합성 저해제 (fluridone)와 EPSP synthase 저해제 (glyphosate) 처리에서는 CA와 wild type간의 반응차이가 관찰되지 않았다. Paraquat와 diuron을 이용한 약광 조건의 실험에서도 온실조건의 실험결과와 유사한 정도로 paraquat처리에 대해서만 내성을 나타내었다. 온실조건에서의 엽위별 반응의 경우, 6 - 9 엽기 식물체에 paraquat를 처리하였을 때, 약제처리 당시 위로부터 3 - 4번째 전개되고 있었던 잎이 상대적으로 내성 정도가 높게 나타났다. 한편 paraquat 처리 시에 여러 농도의 ascorbic acid를 혼합할 경우, CA와 wild type 모두에서 비슷한 정도로 paraquat 활성을 경감시켰다. 결론적으로, CuZnSOD/APX의 과대발현은 photosystem I 에서 발생되는 산화스트레스에 대해서만 주로 작용하며, 다른 제초제들에 의해 발생되는 산화적 스트레스에 대해서는 소거 능력이 부족한 것으로 판단되었다.

Antioxidative responses of transgenic tobacco plants expressing both superoxide dismutase (SOD) and ascorbate peroxidase (APX) in chloroplasts was investigated with several herbicides. In greenhouse test, tolerance of SOD/APX-overexpressed tobacco (CA) to photosystem (PS) I inhibitor paraquat was increased by about 40%. However, any response differences between CA and wild type (WT) tobacco was not observed in a treatment with PS II inhibitors (bromoxynil, diuron and bromacil), chlorophyll biosynthesis inhibitor(oxyfluorfen), carotenoid biosynthesis inhibitor (fluridone) and 5-enolpyruvylshikimate-3-phosphate (EPSP) synthase inhibitor (glyphosate). This tendency was also similar in the growth chamber test of low light intensity, using paraquat and diuron. That is, increased antioxidant activity of CA was shown only in paraquat treatment. When paraquat was foliar-treated to 6 to 9-leaf stage plant, the third to fourth placed leaf from shoot tip showed relatively higher antioxidant activity. Ascorbate supplemented to paraquat solution alleviated the phytotoxicity with a similar range in both CA and WT. In conclusion, CA specifically responded to oxidative stress induced by paraquat among tested herbicides in a whole plant assay.

키워드

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피인용 문헌

  1. Increase of resistance to oxidative stress induced by methyl viologen in progeny from a cross between two transgenic Petunia lines with NDPK and SOD genes vol.38, pp.3, 2011, https://doi.org/10.5010/JPB.2011.38.3.215
  2. Enhanced tolerance to oxidative stress in transgenic tobacco plants expressing three antioxidant enzymes in chloroplasts vol.26, pp.5, 2007, https://doi.org/10.1007/s00299-006-0253-z
  3. Resistance of SOD2-transgenic petunia line to oxidative stress vol.37, pp.4, 2010, https://doi.org/10.5010/JPB.2010.37.4.562
  4. Modification of reactive oxygen species scavenging capacity of chloroplasts through plastid transformation vol.76, pp.3-5, 2011, https://doi.org/10.1007/s11103-011-9784-y