Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Protective Effect of Rehmannia Radix Preparata Extract on the Cisplatin-induced Cytotoxicity of HEI-OC1 Cells via Scavenging of Free Radicals

숙지황 추출물이 Cisplatin으로 손상된 HEI-OC1 세포보호와 유리라디칼 소거능에 미치는 영향

  • Yu Hyeon-Hee (Department of Food and Nutrition, Kunsan National University) ;
  • Seo Se-Jeong (Department of Oral Biochemistry & Vestibulocochlear Research Center, School of Dentistry, Wonkwang University) ;
  • Kim Yeon-Hwa (Department of Oral Biochemistry & Vestibulocochlear Research Center, School of Dentistry, Wonkwang University) ;
  • Park Rae-Kil (Vestibulocochlear Research Center & Department of Microbiology, School of Medicine, Wonkwang University) ;
  • So Hong-Seob (Vestibulocochlear Research Center & Department of Microbiology, School of Medicine, Wonkwang University) ;
  • Jeon Byung-Hun (Department of Oriental Pathology, College of Oriental Medicine, Wonkwang University) ;
  • Shin Mee-Kyung (Department of Food and Nutrition, Wonkwang University) ;
  • Jung Su-Young (Department of Food and Nutrition, Wonkwang University) ;
  • Kim Ki-Young (Department of Beauty Design, Wonkwang University) ;
  • You Yong-Ouk (Department of Oral Biochemistry & Vestibulocochlear Research Center, School of Dentistry, Wonkwang University)
  • 유현희 (군산대학교 식품영양학과) ;
  • 서세정 (원광대학교 치과대학 구강생화학교실.VCRC) ;
  • 김연화 (원광대학교 치과대학 구강생화학교실.VCRC) ;
  • 박래길 (원광대학교 의과대학 미생물학과.VCRC) ;
  • 소홍섭 (원광대학교 의과대학 미생물학과.VCRC) ;
  • 전병훈 (원광대학교 한의과대학 병리학교실) ;
  • 신미경 (원광대학교 식품영양학과) ;
  • 정수영 (원광대학교 식품영양학과) ;
  • 김기영 (원광대학교 뷰티디자인학부) ;
  • 유용욱 (원광대학교 치과대학 구강생화학교실.VCRC)
  • Published : 2005.10.01
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Abstract

The steamed root of Rehmannia glutinosa has been used for treatment of inner ear diseases, such as tinnitus and hearing loss in traditional Oriental Medicine. In the present study, we investigated the effect of ethanol extract of steamed root R. glutinosa (SRG) on cisplatin cytotoxicity of HEI-OC1 auditory cells. In addition, to investigate the mechanism of SRG on cisplatin cytotoxicity, the effects of SRG on lipid peroxidation as well as scavenging activities against various free radicals were measured in cisplatin-treated cells. Treatment of SRG protected cells from cisplatin and reduced lipid peroxidation in a dose-dependent manner. Furthermore, SRG demonstrated significant scavenging activity against various free radicals, including superoxide radical, hydroxyl radical, hydrogen peroxide, and DPPH radical. These results indicate that SRG protects cisplatin-induced damages of HEI-OC1 cells through inhibition of lipid peroxidation and augmenting scavenging activities against free radials.

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